SPSC | Sucrose phosphate synthase isoform C

AS15 2997 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

SPSC | Sucrose phosphate synthase isoform C

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPS4 sequence, Uniprot: F4JLK2, TAIR: AT4G10120.

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1: 1000 (WB)

Expected | apparent MW 118,88 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Corchorus olitorius (A0A1R3I4K1), Gossypium hirsutum (A0A1U8K702)

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Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application example

Proteins were isolated from Arabidopsis thaliana wilde-type and SPS mutants were solubilized with 3X LB (6 M urea, 12% SDS, 30% glycerol, 100 mM DTT, 150 mM Tris pH7.0, 0.8% Comassie G-250). 10 µg of proteins were loaded into each lane and separated on 10% SDS-PAGE, and then blotted overnight onto PVDF membrane. Membranes were blocked with milk powder for 2 h and then incubated in the primary antibody solution at 1: 1 000 overnight at 4°C with agitation, which was then decanted and the membrane was washed 3 times for 5 min in TBST. Membrane was incubated at RT for 1 hour in 1:10 000 Agrisera goat anti-Rabbit secondary antibody (AS09 602), followed by washing steps as above. Membrane was developed for 2 min with chemiluminescent detection reagent, according to the manufacturer’s instructions and recorded using FujiFilm CCD camera with 10 s increment time for around 5 min.

Additional information

Related products

Secondary antibodies

Background

SPSC is a protein involved in the photosynthetic sucrose synthesis, as a catalyst of the rate-limiting step of from UDP-glucose and fructose- 6-phosphate. It is also a part of the regulation of carbon partioning in plant leaves.
Alternative names: SPS4, SPS4F, Sucrose phosphate synthase 4F, UDP-glucose-fructose-phosphate glucosyltransferase