Anti-SPSC | Sucrose phosphate synthase isoform C

Product no: AS15 2997

AS15 2997  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Phaseolus vulgaris

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  • Data sheet
  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPS4 sequence, Uniprot: F4JLK2, TAIR: AT4G10120.

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1: 1000 (WB)
    Expected | apparent MW: 118.88 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Phaseolus vulgaris
    Predicted reactivity: Corchorus olitorius (A0A1R3I4K1), Gossypium hirsutum (A0A1U8K702)

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example
    Western blot using anti-SPSC antibody
    Proteins were isolated from Arabidopsis thaliana wilde-type and SPS mutants were solubilized with 3X LB (6 M urea, 12% SDS, 30% glycerol, 100 mM DTT, 150 mM Tris pH7.0, 0.8% Comassie G-250). 10 µg of proteins were loaded into each lane and separated on 10% SDS-PAGE, and then blotted overnight onto PVDF membrane. Membranes were blocked with milk powder for 2 h and then incubated in the primary antibody solution at 1: 1 000 overnight at 4°C with agitation, which was then decanted and the membrane was washed 3 times for 5 min in TBST. Membrane was incubated at RT for 1 hour in 1:10 000 Agrisera goat anti-Rabbit secondary antibody (AS09 602), followed by washing steps as above. Membrane was developed for 2 min with chemiluminescent detection reagent,  according to the manufacturer’s instructions and recorded using FujiFilm CCD camera with 10 s increment time for around 5 min.
  • Background
  • Background:

    SPSC is a protein involved in the photosynthetic sucrose synthesis, as a catalyst of the rate-limiting step of from UDP-glucose and fructose- 6-phosphate. It is also a part of the regulation of carbon partioning in plant leaves.
    Alternative names: SPS4, SPS4F, Sucrose phosphate synthase 4F, UDP-glucose-fructose-phosphate glucosyltransferase

  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
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AS06 185   |  Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Alfalfa, Z.mays, S.lycopersicum   | cellular [compartment marker] of cytoplasm

319 €
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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
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AS03 035A |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for di and monocots  |  cellular [compartment marker] of cytoplasm for photosynthetic tissues.

Benefits of using this antibody

319 €
Add to cart
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items