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SPSC | Sucrose phosphate synthase isoform C

AS15 2997  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

SPSC | Sucrose phosphate synthase isoform C in the group Antibodies for Plant/Algal  / Photosynthesis  / Sucrose metabolism at Agrisera AB (Antibodies for research) (AS15 2997)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPS4 sequence, Uniprot: F4JLK2, TAIR: AT4G10120.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format

Lyophilized

Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1: 1000 (WB)
Expected | apparent MW

118.88 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Corchorus olitorius (A0A1R3I4K1), Gossypium hirsutum (A0A1U8K702)

Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples
Application example
Western blot using anti-SPSC antibody
Proteins were isolated from Arabidopsis thaliana wilde-type and SPS mutants were solubilized with 3X LB (6 M urea, 12% SDS, 30% glycerol, 100 mM DTT, 150 mM Tris pH7.0, 0.8% Comassie G-250). 10 µg of proteins were loaded into each lane and separated on 10% SDS-PAGE, and then blotted overnight onto PVDF membrane. Membranes were blocked with milk powder for 2 h and then incubated in the primary antibody solution at 1: 1 000 overnight at 4°C with agitation, which was then decanted and the membrane was washed 3 times for 5 min in TBST. Membrane was incubated at RT for 1 hour in 1:10 000 Agrisera goat anti-Rabbit secondary antibody (AS09 602), followed by washing steps as above. Membrane was developed for 2 min with chemiluminescent detection reagent,  according to the manufacturer’s instructions and recorded using FujiFilm CCD camera with 10 s increment time for around 5 min.

Additional information

Related products

Related products
AS06 185 | Anti-SPS | Sucrose phosphate synthase (maize), rabbit antibodies
AS03 035A | Anti-SPS | Sucrose phosphate synthase, global, rabbit antibodies
AS03 035-HRP | Anti-SPS | Sucrose phosphate synthase, global (40 µg, HRP-conjugated), rabbit antibodies
AS03 035-ALP | Anti-SPS | Sucrose phosphate synthase, global (40 µg, ALP-conjugated), rabbit antibodies
AS15 2995 | Anti-SPSA1 | Sucrose phosphate synthase isoform A1, rabbit antibodies
AS15 2996 | Anti-SPSA2 | Sucrose phosphate synthase isoform A1, rabbit antibodies

Plant and algal protein extraction buffer

Background

Background

SPSC is a protein involved in the photosynthetic sucrose synthesis, as a catalyst of the rate-limiting step of from UDP-glucose and fructose- 6-phosphate. It is also a part of the regulation of carbon partioning in plant leaves.
Alternative names: SPS4, SPS4F, Sucrose phosphate synthase 4F, UDP-glucose-fructose-phosphate glucosyltransferase

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