SPSC | Sucrose phosphate synthase isoform C
AS15 2997 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPS4 sequence, Uniprot: F4JLK2, TAIR: AT4G10120.
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 µl
Reconstitution
For reconstitution add 50 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1: 1000 (WB)
Expected | apparent MW
118.88 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Corchorus olitorius (A0A1R3I4K1), Gossypium hirsutum (A0A1U8K702)
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
Application example

Proteins were isolated from Arabidopsis thaliana wilde-type and SPS mutants were solubilized with 3X LB (6 M urea, 12% SDS, 30% glycerol, 100 mM DTT, 150 mM Tris pH7.0, 0.8% Comassie G-250). 10 µg of proteins were loaded into each lane and separated on 10% SDS-PAGE, and then blotted overnight onto PVDF membrane. Membranes were blocked with milk powder for 2 h and then incubated in the primary antibody solution at 1: 1 000 overnight at 4°C with agitation, which was then decanted and the membrane was washed 3 times for 5 min in TBST. Membrane was incubated at RT for 1 hour in 1:10 000 Agrisera goat anti-Rabbit secondary antibody (AS09 602), followed by washing steps as above. Membrane was developed for 2 min with chemiluminescent detection reagent, according to the manufacturer’s instructions and recorded using FujiFilm CCD camera with 10 s increment time for around 5 min.
Additional information
Background
Background
SPSC is a protein involved in the photosynthetic sucrose synthesis, as a catalyst of the rate-limiting step of from UDP-glucose and fructose- 6-phosphate. It is also a part of the regulation of carbon partioning in plant leaves.
Alternative names: SPS4, SPS4F, Sucrose phosphate synthase 4F, UDP-glucose-fructose-phosphate glucosyltransferase
Product citations
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