SPSC | Sucrose phosphate synthase isoform C

AS15 2997 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Background		SPSC is a protein involved in the photosynthetic sucrose synthesis, as a catalyst of the rate-limiting step of from UDP-glucose and fructose- 6-phosphate. It is also a part of the regulation of carbon partioning in plant leaves. Alternative names: SPS4, SPS4F, Sucrose phosphate synthase 4F, UDP-glucose-fructose-phosphate glucosyltransferase
Immunogen		KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPS4 sequence, Uniprot: F4JLK2, TAIR: AT4G10120.
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		50 µl
Reconstitution		For reconstitution add 50 µl of sterile water.
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Related products		AS06 185 \| anti-SPS \| Sucrose phosphate synthase (maize) AS03 035A \| anti-SPS \| Sucrose phosphate synthase, global AS03 035-HRP \| anti-SPS \| Sucrose phosphate synthase, global (40 µg, HRP-conjugated) AS03 035-ALP \| anti-SPS \| Sucrose phosphate synthase, global (40 µg, ALP-conjugated) AS15 2995 \| anti-SPSA1 \| Sucrose phosphate synthase isoform A1, rabbit antibody AS15 2996 \| anti-SPSA2 \| Sucrose phosphate synthase isoform A1, rabbit antibody Plant and algal protein extraction buffer Secondary antibodies
Additional information

application information
Recommended dilution
Expected \| apparent MW		118.88 kDa
Confirmed reactivity		Corchorus olitorius
Predicted reactivity		Arabidopsis thaliana
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references		To be added when available antibody released in June 2016.

Application example

Proteins were isolated from Arabidopsis thaliana wilde-type and SPS mutants were solubilized with 3X LB (6 M urea, 12% SDS, 30% glycerol, 100 mM DTT, 150 mM Tris pH7.0, 0.8% Comassie G-250). 10 µg of proteins were loaded into each lane and separated on 10% SDS-PAGE, and then blotted overnight onto PVDF membrane. Membranes were blocked with milk powder for 2 h and then incubated in the primary antibody solution at 1: 1 000 overnight at 4°C with agitation, which was then decanted and the membrane was washed 3 times for 5 min in TBST. Membrane was incubated at RT for 1 hour in 1:10 000 Agrisera goat anti-Rabbit secondary antibody (AS09 602), followed by washing steps as above. Membrane was developed for 2 min with chemiluminescent detection reagent, according to the manufacturer’s instructions and recorded using FujiFilm CCD camera with 10 s increment time for around 5 min.

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