SRP43 | Signal recognition peptide 43

Product no: AS12 2105

AS12 2105 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

 

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SRP43,UniProt: O22265, TAIR: AT2G47450

    Host: Chicken
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution:

    For reconstitution add 50 µl of sterile water

    Storage:

    Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

    Tested applications: Western blot (WB)
    Recommended dilution:

    1 : 5000 (WB)

    Expected | apparent MW:

    41.3 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Not reactive in:

    no confirmed exceptions from predicted reactivity are currently known

  • Application Examples
  • Western blot using anti-SRP43 antibodies

    15 µg/well of total protein extracted freshly from rosetta leaves of Arabidopsis thaliana with 2X Laemmli buffer and denatured at 95 °C/5 min. Samples were separated in the cold on 12% SDS-PAGE and blotted for 1.5 h to nitrocellulose (pore size of 0.45 µm), using: wet, semi-dry transfer in the cold. Blot was blocked with 5 % milk for: 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in TBS for ON/4°C with agitation. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed once for 5 min in TBS-T (1% Tween-20) and 2 times for 10 min in TBS at RT with agitation. Blot was incubated in matching secondary antibody (anti-chicken IgG horse radish peroxidase conjugated AS10 1489, Agriera) diluted to 1: 5000 in for 1.5 h/RT with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent: AS16 ECL-N-10 AgriseraBright (Agrisera). Exposure time was 1 minute.

    Courtesy od dr. Peng Wang, School of Biological Sciences, The University of Hong Kong

  • Background
  • Background:

    SRP43 (Signal recognition peptide 43) is a part of the chloroplast signal recognition particle pathway, required for post-translational targeting of proteins into the thylakoid membrane but seems to be dispensable for co-translational targeting with a translating ribosome present. The protein acts as a highly specific chaperone for LHCPs, preventing aggregation and being able to dissolve aggregates.
    Alternative names: Chloroplast signal recognition peptide 43, CPSRP43, CAO, CHAOS

  • Protocols


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