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Staphylococcal Protein A, FITC-conjugated

AS20 4471 | Clonality: Polyclonal  Host: Chicken Reactivity: Staphylococcus aureus Protein A

Staphylococcal Protein A, FITC-conjugated in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS20 4471)

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Product Information

Immunogen

Recombinant Protein A 

Host Chicken
Clonality Polyclonal
Purity Affinity purified on antigen
Format Liquid in PBS‚ 50% Glycerol with 0.075% sodium azide, fluorescein conjugated
Quantity 0.5 mg
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Immunohistochemistry (IHC)
Recommended dilution To be determined by the end user.

Reactivity

Confirmed reactivity Staphylococcus aureus

Application examples

Additional information

Additional information

Antibodies were isolated from immune eggs, affinity purified on a Protein A column and conjugated to fluoroscein isothiocyanate (FITC).

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Background

Background

Protein A is a surface protein of S. aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

Product citations

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