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Staphylococcal Protein A, agarose-conjugated

AS20 4470 | Clonality: Polyclonal  Host: Chicken Reactivity: Staphylococcus aureus

Staphylococcal Protein A, agarose-conjugated in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS20 4470)

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Product Information

Immunogen Recombinant Protein A
Host Chicken
Clonality Polyclonal
Purity Affinity Purified on antigen
Format Antibody Liquid in PBS‚ pH 7.2 with 0.075% sodium azide coupled to immunoprecipiation gel
Quantity 2 ml  bed volume
Storage Store at +4-8°C. Do not freeze. 
Tested applications Immuno affinity chromatography

Reactivity

Confirmed reactivity  Staphylococcus aureus

Application examples

Additional information

Additional information Antibodies were isolated from immune eggs, affinity purified on a protein A column and conjugated to N- hydroxysuccinimide (NHS)-activated agarose beads. Beads were washed extensively with after the blocking of the residual NHS sites.
The specific activity of the anti-protein A-agarose was determined. For this lot, 1.1 mg protein A bound per ml agarose.

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Background

Background

Protein A is a surface protein of S. aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

Product citations

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