Staphylococcal Protein A, agarose-conjugated

AS20 4470 | Clonality: Polyclonal | Host: Chicken | Reactivity: Staphylococcus aureus

Product Information

Immunogen Recombinant Protein A

Host Chicken

Clonality Polyclonal

Purity Immunogen affinity purified in PBS pH 7.2. Contains 0.075% sodium azide, coupled to immunoprecipiation gel.

Format Liquid

Quantity 2 ml bed volume

Storage Store at +4-8°C. Do not freeze.

Tested applications Immuno affinity chromatography

Reactivity

Confirmed reactivity Staphylococcus aureus

Application examples

Additional information

Additional information Antibodies were isolated from immune eggs, affinity purified on a protein A column and conjugated to N- hydroxysuccinimide (NHS)-activated agarose beads, Beads were washed extensively with after the blocking of the residual NHS sites

The specific activity of the anti-protein A-agarose was determined, For this lot, 1,1 mg protein A bound per ml agarose

Background

Protein A is a surface protein of S. aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

Product citations

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background:	Protein A is a surface protein of S. aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
Confirmed reactivity:	Staphylococcus aureus
additional information:	Antibodies were isolated from immune eggs, affinity purified on a protein A column and conjugated to N- hydroxysuccinimide (NHS)-activated agarose beads, Beads were washed extensively with after the blocking of the residual NHS sites
additional information (application):	The specific activity of the anti-protein A-agarose was determined, For this lot, 1,1 mg protein A bound per ml agarose
Clonality:	Polyclonal
Format:	Liquid
Host:	Chicken
immunogen:	Recombinant Protein A
Purity:	Immunogen affinity purified in PBS pH 7.2. Contains 0.075% sodium azide, coupled to immunoprecipiation gel.
Quantity:	2 ml bed volume
storage:	Store at +4-8°C. Do not freeze.
tested applications:	Immuno affinity chromatography