Staphylococcal Protein A, agarose-conjugated

Product no: AS20 4470

AS20 4470 | Clonality: Polyclonal  Host: Chicken Reactivity: Staphylococcus aureus

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  • Product Info
  • Immunogen: Recombinant Protein A
    Host: Chicken
    Clonality: Polyclonal
    Purity: Immunogen affinity purified in PBS pH 7.2. Contains 0.075% sodium azide, coupled to immunoprecipiation gel.
    Format: Liquid
    Quantity: 2 ml bed volume
    Storage: Store at +4-8°C. Do not freeze.
    Tested applications: ImmunoAffinity chromatography (IAC)
  • Reactivity
  • Confirmed reactivity: Protein A from Staphylococcus aureus
  • Additional Information
  • Additional information: Antibodies were isolated from immune eggs, affinity purified on a protein A column and conjugated to N- hydroxysuccinimide (NHS)-activated agarose beads, Beads were washed extensively with after the blocking of the residual NHS sites
    Additional information (application): The specific activity of the anti-protein A-agarose was determined, For this lot, 1,1 mg protein A bound per ml agarose
  • Background
  • Background:

    Protein A is a surface protein of S. aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

  • Protocols
  • Antibody Protocols
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AS20 4471 | Clonality: Polyclonal  Host: Chicken Reactivity: Staphylococcus aureus Protein A

252 €
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