Strep-tag®II-Tag (N-terminal) (clone 2G2)

AS22 4735 | Clonality: Monoclonal | Host: Mouse | Reactivity: Strep tag (N-terminal)

Product Information

Immunogen Recombinant human protein containing three tandem repeated Strep II tags (3xStrep-tag), separated by GlySer-linker sequence, at the N-terminus (expressed in HEK293 cells).

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Purified on Protein A

Format Liquid in PBS at 0.5 mg/ml.

Quantity 100 µg

Storage Store at 4°C for 1-2 weeks (short term). For log term storage, aliquot and store at -20°C and below, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1: 1000 (ELISA), 1: 5000 (WB)

Expected | apparent MW Depends upon fusion partner.

Reactivity

Confirmed reactivity Artificial sequence (3x WSHPQFEKGS)

Application examples

Additional information

Background

Background Strep-tag®II is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys coded also as WSHPQFEKGS) and can be N- or C- terminally fused to recombinant proteins.

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background:	Strep-tag®II is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys coded also as WSHPQFEKGS) and can be N- or C- terminally fused to recombinant proteins.
calculated \| apparent molecular mass [kDa]:	Depends upon fusion partner.
Clonality:	Monoclonal
Format:	Liquid in PBS at 0.5 mg/ml.
Host:	Mouse
immunogen:	Recombinant human protein containing three tandem repeated Strep II tags (3xStrep-tag), separated by GlySer-linker sequence, at the N-terminus (expressed in HEK293 cells).
Purity:	Purified on Protein A
Quantity:	100 µg
recommended dilution:	1: 1000 (ELISA), 1: 5000 (WB)
storage:	Store at 4°C for 1-2 weeks (short term). For log term storage, aliquot and store at -20°C and below, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Sub class:	IgG1
tested applications:	ELISA (ELISA), Western blot (WB)
Confirmed reactivity:	Artificial sequence (3x WSHPQFEKGS)