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SUN1 | SUN domain-containing protein 1

AS18 4224   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana

SUN1 | SUN domain-containing protein 1  in the group Antibodies for Plant/Algal  / Compartment Markers / Nuclear marker at Agrisera AB (Antibodies for research) (AS18 4224)

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Product Information

Immunogen KLH-conjugated peptide derived from N-terminus of SUN1 of Arabidopsis thaliana UniProt: Q9FF75  , TAIR: AT5G04990
Host

Rabbit

Clonality Polyclonal
Purity Affinity purified serum, in PBS pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 51 | 56 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Arabis nemor, Eutrema salsugineum, Microthlaspi erraticum, Noccaea caerulescens

Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot using anti-SUN1 antibodies

Samples:
1 - 15 ug of Arabidopsis thaliana Col-0 callus total protein
2 - 15 ug of Arabidopsis thaliana sun1 mutant callus total protein
3 - 15 ug of Arabidopsis thaliana sun1 sun2 mutant callus total protein
MW markers: PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa (ThermoFisherScientific)

15 µg/well of total protein extracted freshly from Arabidopsis thaliana callus with 50 mM Tris pH7.5, 150 mM NaCl, 1 mM EGTA, 1 mM DTT, 1% TritonX-100, 0.5% sodium deoxycholate, 0.1% SDS, 1xprotease inhibitor cocktail (ThermoFisherScientific) and denatured with Bio-Rad 1x Laemmli sample buffer (BioRad) at 95°C 5 min. Samples were separated on 12% SDS-PAGE  and blotted 15V, 16h to PVDF (pore size of  45 µm), using wet transfer. Blot was blocked with 5% BSA for 1h/RT agitation. Blot was incubated in the primary antibody in 2% BSA in TBS-Tat a dilution of 1: 1 000 for 1h/RT with agitation in 2% BSA with TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:25 000 in TBS-T for 1h/RT with agitation. The blot was washed as above and developed for 2 min with Agrisera ECLBright. Exposure time was 10 seconds.

Courtesy of Wei Wang, Umeå Plant Science Centre, Sweden

Additional information

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Background

Background SUN1 (nuclear envelope protein) is a member of the Sad1/UNC-84 (SUN)-domain proteins.SUN domain proteins are part of the cytoskeletal-nucleoskeletal bridging complexes. These proteins are localized to the nuclear envelope and are present as homomers and heteromers in vivo. Involved in maintaining the elongated nuclear shape of epidermal cells.

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