T7 epitope tag

AS21 4679 | Clonality: Monoclonal | Host: Mouse | Reactivity: T7-tagged proteins

Product Information

Immunogen

T7 epitope tag, sequence: MASMTGGQQMG

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1a

Purity Affinity purified in PBS pH 7.4. Contains 0.02 % sodium azide. Contains 50 % glycerol.

Format Liquid

Quantity 50 µg

Storage Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1: 500 - 1: 5000 (WB)

Expected | apparent MW Depends upon protein fusion partner

Reactivity

Confirmed reactivity T7 epitope tag

Application examples

Additional information

Background

Background T7 epitope tag consists of 11 amino acids in the leader sequence of T7 bacteriophage gene10 and is used as a tag in many expression vectors including pET system. Specific anti-T7 tag antibodies are suitable for detection of T7-tagged proteins.

Product citations

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Confirmed reactivity:	T7 epitope tag
background:	T7 epitope tag consists of 11 amino acids in the leader sequence of T7 bacteriophage gene10 and is used as a tag in many expression vectors including pET system. Specific anti-T7 tag antibodies are suitable for detection of T7-tagged proteins.
calculated \| apparent molecular mass [kDa]:	Depends upon protein fusion partner
Clonality:	Monoclonal
Format:	Liquid
Host:	Mouse
immunogen:	T7 epitope tag, sequence: MASMTGGQQMG
Purity:	Affinity purified in PBS pH 7.4. Contains 0.02 % sodium azide. Contains 50 % glycerol.
Quantity:	50 µg
recommended dilution:	1: 500 - 1: 5000 (WB)
storage:	Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Sub class:	IgG1a
tested applications:	ELISA (ELISA), Western blot (WB)