TPS1 | Trehalose-6-phosphate synthase 1

Product no: AS12 2635

AS12 2635 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana TPS1, UniProt: Q9SYM4, TAIR: AT1G78580

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 5000 (WB)
    Expected | apparent MW: 105,9 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Camelia sinensis, Vitis vinifera
    Species of your interest not listed? Contact us
    Not reactive in: Cucumis sativus, Monocots
  • Application Examples
  • Application example

    western blot using anti-TPS1 antibodies

    Total protein from Arabidopsis thaliana wilde type (1,2), knowck out mutant (3) and overexpression line (4) were extracted with 50 mM HEPES/NaOH (pH 7.5), 1 mM EDTA, 1 mM DTT (+Protease Inhibitor cocktail) and denatured at 100°C for 5 min. Proteins were separated on 10 % SDS-PAGE and blotted overnight at 4°C to PVDF using wet tank transfer. Blots were blocked with 5 % low fat milk in 1xTBS-T for 1h at RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight at 4°C with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in 2 % low fat milk in 1xTBS for 1h at RT with agitation. The blot was washed as above and developed. Exposure time was 10 seconds.

    Courtesy of Dr. Vasiliki Zacharaki, Umeå Plant Science Centre, Sweden


    western blot using anti-TPS1 antibodies

    10 µg of total protein from Nicotiana benthamiana leaves expressing AtTPS1-GFP were separated on 10% SDS-PAGE using semi-dry transfer and blotted 1h to PVDF. Crude: non-purified material. Purified: immunoprecipitated with anti-GFP beads. Blots were blocked with 5% skimmed milk powder dissolved in TBS-T (0.1 % Tween 20)  at 4°C ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 for 1h at room temperature (RT) with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 25 000 for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was 30 seconds.

    Courtesy of Dr. Takeo Sato, Hokkaido University, Japan

  • Additional Information
  • Additional information (application): So far this antibody was not used on endogenous extracts from Arabidopsis thaliana
  • Background
  • Background:

    TPS1 (Trehalose-6-phosphate synthase 1), EC=2.4.1.15 is an enzyme required for normal embryo development, vegetative growth and transition to flowering. It is involved in the regulation of glucose sensing. Expressed in seedlings, leaves, roots, stems, flowers and siliques. Up-regulated during seed development. Alternative names: alpha-trehalose-phosphate synthase [UDP-forming] 1.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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Accessories

AS05 086  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, C. annuum, C. sativus, F. margarita Swingle, F. arundinacea,  H. vulgare, L. esculentum, L. chilense, Malus x domestica Borkh. c.v. Fuji, M. polymorpha, Medicago truncatula, N. tabacum, O. sativa, P. glauca, Populus sp., S. tuberosum, S. sogarandinum, Triticum |  cellular [compartment marker] of cytoplasm

Replaced by AS14 2813 

319 €

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
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AS09 607 |  Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)

218 €
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