TPS1 | Trehalose-6-phosphate synthase 1

Name: TPS1 | Trehalose-6-phosphate synthase 1
SKU: AS12 2635
Price: 302 EUR
Availability: InStock

AS12 2635 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

TPS1 | Trehalose-6-phosphate synthase 1 in the group Antibodies Plant/Algal / Plant Developmental Biology / Plant Signal Transduction at Agrisera AB (Antibodies for research) (AS12 2635)

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana TPS1, UniProt: Q9SYM4, TAIR: AT1G78580

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 5000 (WB)

Expected | apparent MW 105,9 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Camelia sinensis, Vitis vinifera
Species of your interest not listed? Contact us

Not reactive in Cucumis sativus, Monocots

Application examples

Application example

Total protein from Arabidopsis thaliana wilde type (1,2), knowck out mutant (3) and overexpression line (4) were extracted with 50 mM HEPES/NaOH (pH 7.5), 1 mM EDTA, 1 mM DTT (+Protease Inhibitor cocktail) and denatured at 100°C for 5 min. Proteins were separated on 10 % SDS-PAGE and blotted overnight at 4°C to PVDF using wet tank transfer. Blots were blocked with 5 % low fat milk in 1xTBS-T for 1h at RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight at 4°C with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in 2 % low fat milk in 1xTBS for 1h at RT with agitation. The blot was washed as above and developed. Exposure time was 10 seconds.

Courtesy of Dr. Vasiliki Zacharaki, Umeå Plant Science Centre, Sweden

10 µg of total protein from Nicotiana benthamiana leaves expressing AtTPS1-GFP were separated on 10% SDS-PAGE using semi-dry transfer and blotted 1h to PVDF. Crude: non-purified material. Purified: immunoprecipitated with anti-GFP beads. Blots were blocked with 5% skimmed milk powder dissolved in TBS-T (0.1 % Tween 20) at 4°C ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 for 1h at room temperature (RT) with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 25 000 for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was 30 seconds.

Courtesy of Dr. Takeo Sato, Hokkaido University, Japan

Additional information

So far this antibody was not used on endogenous extracts from Arabidopsis thaliana

Background

TPS1 (Trehalose-6-phosphate synthase 1), EC=2.4.1.15 is an enzyme required for normal embryo development, vegetative growth and transition to flowering. It is involved in the regulation of glucose sensing. Expressed in seedlings, leaves, roots, stems, flowers and siliques. Up-regulated during seed development. Alternative names: alpha-trehalose-phosphate synthase [UDP-forming] 1.

Product citations

immunogen:	KLH-conjugated synthetic peptide derived from Arabidopsis thaliana TPS1, UniProt: Q9SYM4, TAIR: AT1G78580
Reconstitution:	For reconstitution add 50 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 5000 (WB)
calculated \| apparent molecular mass [kDa]:	105,9 kDa
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Camelia sinensis, Vitis vinifera Species of your interest not listed? Contact us
not reactive in:	Cucumis sativus, Monocots
Picture (footer):	Application example Total protein from Arabidopsis thaliana wilde type (1,2), knowck out mutant (3) and overexpression line (4) were extracted with 50 mM HEPES/NaOH (pH 7.5), 1 mM EDTA, 1 mM DTT (+Protease Inhibitor cocktail) and denatured at 100°C for 5 min. Proteins were separated on 10 % SDS-PAGE and blotted overnight at 4°C to PVDF using wet tank transfer. Blots were blocked with 5 % low fat milk in 1xTBS-T for 1h at RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight at 4°C with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in 2 % low fat milk in 1xTBS for 1h at RT with agitation. The blot was washed as above and developed. Exposure time was 10 seconds. Courtesy of Dr. Vasiliki Zacharaki, Umeå Plant Science Centre, Sweden 10 µg of total protein from Nicotiana benthamiana leaves expressing AtTPS1-GFP were separated on 10% SDS-PAGE using semi-dry transfer and blotted 1h to PVDF. Crude: non-purified material. Purified: immunoprecipitated with anti-GFP beads. Blots were blocked with 5% skimmed milk powder dissolved in TBS-T (0.1 % Tween 20) at 4°C ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 for 1h at room temperature (RT) with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 25 000 for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was 30 seconds. Courtesy of Dr. Takeo Sato, Hokkaido University, Japan
additional information (application):	So far this antibody was not used on endogenous extracts from Arabidopsis thaliana
background:	TPS1 (Trehalose-6-phosphate synthase 1), EC=2.4.1.15 is an enzyme required for normal embryo development, vegetative growth and transition to flowering. It is involved in the regulation of glucose sensing. Expressed in seedlings, leaves, roots, stems, flowers and siliques. Up-regulated during seed development. Alternative names: alpha-trehalose-phosphate synthase [UDP-forming] 1.