TrxM1/M2 | Thioredoxin M1/M2 (chloroplastic)

AS14 2809 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

TrxM1/M2 | Thioredoxin  M1/M2 (chloroplastic) in the group Antibodies Plant/Algal  / Photosynthesis  / RUBISCO/Carbon metabolism at Agrisera AB (Antibodies for research) (AS14 2809)
TrxM1/M2 | Thioredoxin  M1/M2 (chloroplastic)


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Product Information

Immunogen KLH-conjugated peptide, derived from Arabidopsis thaliana TrxM1 UniProt: O48737, TAIR: AT1G03680 and TrxM2 UniProt:Q9SEU8, TAIR: AT4G03520
Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

20 | 14 kDa


Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica napus, Chlamydomonas reinhardtii, Hordeum vulgare, Oryza sativa, Populus balsamifera, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Theobroma cacao, Zea mays, Viola biflora
Species of your interest not listed? Contact us
Not reactive in Marchantia polymorpha, Physcomitrella patens

Application examples

Application examples

application example

western blot using anti-Trxm antibodies

7.5 and 15 µg of soluble protein extract from WT-Col-0 Arabidopsis thaliana extracted in a buffer containing 50 mM HEPES, 5 mM NaCl and 10 mM MgCl2, separated on 12% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 4% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight in 4°C with agitation. The antibody solution was decanted and the blot was 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in for 2h at RT with agitation. The blot was washed as above and developed for 5min with ECL according to the manufacturer's instructions. Exposure time was 10 min. 

Courtesy of Lauri Nikkanen, University of Turku, Finland

Additional information

5 mM DTT in extraction buffer and 5% B-ME in Lämmli buffer are recommended to use. Samples should be heated at 95°C for 2 min before loading as TRXs proteins have a tendency to oligomerize.

To work with this antibody chloroplast fraction has to be used.

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Background TrxM1/M2 (Thioredoxin M1/M2, chloroplasticT) is a thiol-disulfide oxidoreductase involved in the redox regulation of enzymes of both reductive pentose phosphate pathway (Calvin-Benson cycle) and oxidative pentose phosphate pathway. Under reducing conditions, activates the glyceraldehyde-3-phosphate dehydrogenase and the phosphoribulokinase, and inhibits. the glucose-6-phosphate dehydrogenase. Activates NADP-malate dehydrogenase.

Product citations

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