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TrxM1/M2 | Thioredoxin M1/M2 (chloroplastic)

AS14 2809 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

TrxM1/M2 | Thioredoxin  M1/M2 (chloroplastic) in the group Antibodies for Plant/Algal  / Photosynthesis  / RUBISCO/Carbon metabolism at Agrisera AB (Antibodies for research) (AS14 2809)

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Product Information

Immunogen KLH-conjugated peptide, derived from Arabidopsis thaliana TrxM1 UniProt: O48737, TAIR: AT1G03680 and TrxM2 UniProt:Q9SEU8, TAIR: AT4G03520
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

20 | 14 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica napus, Chlamydomonas reinhardtii, Hordeum vulgare, Oryza sativa, Populus balsamifera, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Theobroma cacao, Zea mays, Viola biflora
Species of your interest not listed? Contact us
Not reactive in Physcomitrella patens

Application examples

Application examples

application example

western blot using anti-Trxm antibodies

7.5 and 15 µg of soluble protein extract from WT-Col-0 Arabidopsis thaliana extracted in a buffer containing 50 mM HEPES, 5 mM NaCl and 10 mM MgCl2, separated on 12% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 4% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight in 4°C with agitation. The antibody solution was decanted and the blot was 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in for 2h at RT with agitation. The blot was washed as above and developed for 5min with ECL according to the manufacturer's instructions. Exposure time was 10 min. 

Courtesy of Lauri Nikkanen, University of Turku, Finland



Additional information

5 mM DTT in extraction buffer and 5% B-ME in Lämmli buffer are recommended to use. Samples should be heated at 95°C for 2 min before loading as TRXs proteins have a tendency to oligomerize.

To work with this antibody chloroplast fraction has to be used.

Related products

Related products

AS14 2808 | Anti-Trxf1/2 | Thioredoxin F1/F2, chloroplastic, rabbit antibodies
AS06 159 | Anti-NtrC | thioredoxin reductase (TR/TRxR), rabbit antibodies

Collection of antibodies to chloroplastic proteins

Plant and algal protein extraction buffer

Background

Background TrxM1/M2 (Thioredoxin M1/M2, chloroplasticT) is a thiol-disulfide oxidoreductase involved in the redox regulation of enzymes of both reductive pentose phosphate pathway (Calvin-Benson cycle) and oxidative pentose phosphate pathway. Under reducing conditions, activates the glyceraldehyde-3-phosphate dehydrogenase and the phosphoribulokinase, and inhibits. the glucose-6-phosphate dehydrogenase. Activates NADP-malate dehydrogenase.

Product citations

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