TrxM1/M2 | Thioredoxin M1/M2 (chloroplastic)
AS14 2809 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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|Recommended dilution||1 : 1000 (WB)|
|Expected | apparent MW||
20 | 14 kDa
|Confirmed reactivity||Arabidopsis thaliana|
|Predicted reactivity||Brassica napus, Chlamydomonas reinhardtii, Hordeum vulgare, Oryza sativa, Populus balsamifera, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Theobroma cacao, Zea mays, Viola biflora|
|Not reactive in||Physcomitrella patens|
|Additional information||5 mM DTT in extraction buffer and 5% B-ME in Lämmli buffer are recommended to use. Samples should be heated at 95°C for 2 min before loading as TRXs proteins have a tendency to oligomerize.
To work with this antibody chloroplast fraction has to be used.
to be added when available, antibody available in October 2015.
7.5 and 15 µg of soluble protein extract from WT-Col-0 Arabidopsis thaliana extracted in a buffer containing 50 mM HEPES, 5 mM NaCl and 10 mM MgCl2, separated on 12% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 4% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight in 4°C with agitation. The antibody solution was decanted and the blot was 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in for 2h at RT with agitation. The blot was washed as above and developed for 5min with ECL according to the manufacturer's instructions. Exposure time was 10 min.
Courtesy of Lauri Nikkanen, University of Turku, Finland
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