TrxM1/M2 | Thioredoxin M1/M2 (chloroplastic)
AS14 2809 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Product Information
20 | 14 kDa
Reactivity
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Application examples
application example
7.5 and 15 µg of soluble protein extract from WT-Col-0 Arabidopsis thaliana extracted in a buffer containing 50 mM HEPES, 5 mM NaCl and 10 mM MgCl2, separated on 12% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 4% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight in 4°C with agitation. The antibody solution was decanted and the blot was 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in for 2h at RT with agitation. The blot was washed as above and developed for 5min with ECL according to the manufacturer's instructions. Exposure time was 10 min.
Courtesy of Lauri Nikkanen, University of Turku, Finland
Additional information
To work with this antibody chloroplast fraction has to be used.
Background
Product citations
Related products: TrxM1/M2 | Thioredoxin M1/M2 (chloroplastic)
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