Trxf1/2 | Thioredoxin F1/F2 (chloroplastic)
AS14 2808 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana

Data sheet | Product citations | Protocols | Customer reviews |
Product Information
Immunogen
KLH-conjugated peptide, derived from Arabidopsis thaliana Trxf1 UniProt: Q9XFH9, TAIR: AT5G16400 and Trxf2 UniProt: Q9XFH8, TAIR: AT3G02730
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified serum in PBS, pH 7.4
Format
Lyophilized
Quantity
50 µg
Reconstitution
For reconstitution add 50 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
19.9 | 12 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Aegilops tauschii, Brassica napus, Chlamydmonas reinhardtii, Fragaria ananassa, Glycine max, Glycine soja, Hyacinthus orientalis, Medicago truncatula, Mesembryanthemum crystallinum, Morus notabilis, Nicotiana tabacum, Oryza sativa, Pisum sativum, Populus trichocarpa, Ricinus communis, Spinacia oleracea, Theobroma cacao, Triticum urartu, Zea mays
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
Physcomitrella patens
Application examples
Application examples
Courtesy of Lauri Nikkanen, University of Turku, Finland
application example
Courtesy of Lauri Nikkanen, University of Turku, Finland
Additional information
5 mM DTT in extraction buffer and 5% B-ME in Lämmli buffer are recommended to use. Samples should be heated at 95°C for 2 min before loading as TRXs proteins have a tendency to oligomerize.
Background
Background
Trxf1/2 (Thioredoxin F1/F2, chloroplastic) involved in the redox regulation of enzymes of Calvin-Benson cycle and oxidative pentose phosphate pathway.
Alternative names: Thioredoxin F1, F2 AtTrxf1, AtTrxf2
Alternative names: Thioredoxin F1, F2 AtTrxf1, AtTrxf2
Product citations
Selected references
Nikkanen et al. (2016). Crosstalk between chloroplast thioredoxin systems in regulation of photosynthesis. Plant Cell Environ. 2016 Aug;39(8):1691-705. doi: 10.1111/pce.12718.
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