Trxf1/2 | Thioredoxin F1/F2 (chloroplastic)

AS14 2808 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana

Product Information

Immunogen KLH-conjugated peptide, derived from Arabidopsis thaliana Trxf1 UniProt: Q9XFH9, TAIR: AT5G16400 and Trxf2 UniProt: Q9XFH8, TAIR: AT3G02730

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum in PBS, pH 7.4

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

19.9 | 12 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Aegilops tauschii, Brassica napus, Chlamydmonas reinhardtii, Fragaria ananassa, Glycine max, Glycine soja, Hyacinthus orientalis, Medicago truncatula, Mesembryanthemum crystallinum, Morus notabilis, Nicotiana tabacum, Oryza sativa, Pisum sativum, Populus trichocarpa, Ricinus communis, Spinacia oleracea, Theobroma cacao, Triticum urartu, Zea mays
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Not reactive in Physcomitrella patens

Application examples

application example

10 or 20 µg of soluble protein extract from WT-Col-0 Arabidopsis thaliana extracted in a buffer containing 50 mM HEPES, 5 mM NaCl and 10 mM MgCl2, separated on 12% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 4% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight in 4°C with agitation. The antibody solution was decanted and the blot was 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in for 2h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 10 min.

Courtesy of Lauri Nikkanen, University of Turku, Finland

Additional information

5 mM DTT in extraction buffer and 5% B-ME in Lämmli buffer are recommended to use. Samples should be heated at 95°C for 2 min before loading as TRXs proteins have a tendency to oligomerize.

Related products

collection of antibodies involced in photosynthesis

Plant and algal protein extraction buffer

Background

Background Trxf1/2 (Thioredoxin F1/F2, chloroplastic) involved in the redox regulation of enzymes of Calvin-Benson cycle and oxidative pentose phosphate pathway.

Alternative names: Thioredoxin F1, F2 AtTrxf1, AtTrxf2

Product citations

Selected references Nikkanen et al. (2016). Crosstalk between chloroplast thioredoxin systems in regulation of photosynthesis. Plant Cell Environ. 2016 Aug;39(8):1691-705. doi: 10.1111/pce.12718.