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Anti-Trxf1/2 | Thioredoxin F1/F2 (chloroplastic)
Product no: AS14 2808
AS14 2808 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana
329
€
Customer reviews
Delivery:
3-6 business days
- Data sheet
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- Product Info
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Immunogen: KLH-conjugated peptide, derived from Arabidopsis thaliana Trxf1 UniProt: Q9XFH9, TAIR: AT5G16400 and Trxf2 UniProt: Q9XFH8, TAIR: AT3G02730 Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 19.9 | 12 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Aegilops tauschii, Brassica napus, Chlamydmonas reinhardtii, Fragaria ananassa, Glycine max, Glycine soja, Hyacinthus orientalis, Medicago truncatula, Mesembryanthemum crystallinum, Morus notabilis, Nicotiana tabacum, Oryza sativa, Pisum sativum, Populus trichocarpa, Ricinus communis, Spinacia oleracea, Theobroma cacao, Triticum urartu, Zea mays
Species of your interest not listed? Contact usNot reactive in: Marchantia polymorpha, Physcomitrella patens - Application Examples
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Application example
10 or 20 µg of soluble protein extract from WT-Col-0 Arabidopsis thaliana extracted in a buffer containing 50 mM HEPES, 5 mM NaCl and 10 mM MgCl2, separated on 12% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 4% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight in 4°C with agitation. The antibody solution was decanted and the blot was 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in for 2h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 10 min.
Courtesy of Lauri Nikkanen, University of Turku, Finland - Additional Information
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Additional information (application): 5 mM DTT in extraction buffer and 5% B-ME in Lämmli buffer are recommended to use, Samples should be heated at 95°C for 2 min before loading as TRXs proteins have a tendency to oligomerize - Background
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Background: Trxf1/2 (Thioredoxin F1/F2, chloroplastic) involved in the redox regulation of enzymes of Calvin-Benson cycle and oxidative pentose phosphate pathway.
Alternative names: Thioredoxin F1, F2 AtTrxf1, AtTrxf2 - Product Citations
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Selected references: Nikkanen et al. (2016). Crosstalk between chloroplast thioredoxin systems in regulation of photosynthesis. Plant Cell Environ. 2016 Aug;39(8):1691-705. doi: 10.1111/pce.12718. - Protocols
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- Reviews:
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Bettina Mathes | 2016-06-23With a 1:1000 dilution, 4% Milk in 1xTBS with 0.05% Tween (30 minutes blocking) the antibody works fine on pea stroma and chloroplasts and with Arabidopsis chloroplasts. It's only important to cook the samples before for 3 minutes at 95°C. Incubation 2h at roomtemperature is enough.
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