Tubulin alpha chain, FITC conjugated (clone TU-01)

AS21 4626 | Clonality: Monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana, Eisenia, Nicotiana tabacum, Paramecium, Saccharomyces cerevisiae, human, mouse, pig, turkey

Tubulin alpha chain, FITC conjugated (clone TU-01)

Product Information

Immunogen Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization,

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Conjugated with fluorescein isothiocyanate (FITC) under optimum conditions, The conjugate is purified by size-exclusion chromatography.

Format Liquid at 1 mg/ml.

Quantity 0,1 mg

Storage Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunocytochemistry (ICC), Flow cytometry (Flowcyt)

Recommended dilution 1-4 µg/ml (Flowcyt)

Expected | apparent MW 50 | 54 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Eisenia, Nicotiana tabacum, Paramecium, Saccharomyces cerevisiae, human, mouse, pig, turkey

Predicted reactivity Species of your interest not listed? Contact us

Application examples

Application examples Immunocytochemistry using anti-tubulin alpha chain monoclonal antibodies, FITC conjugated

HeLa cells stained with alpha tubulin monoclonal antibodies, conjugated with FITC.

Additional information

Additional information This antibody is recognizing a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin 1, UniProt: Q71U36

Background

Tubulin alpha (TUA) together with beta tubulin is making up microtubules.

The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport.

Product citations

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