Tubulin alpha chain, FITC conjugated (clone TU-01)

Product no: AS21 4626
AS21 4626 | Clonality: Monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana, Eisenia, Nicotiana tabacum, Paramecium, Saccharomyces cerevisiae, human, mouse, pig, turkey
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  • Product Info
  • Sub class: IgG1
    Immunogen: Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization,
    Host: Mouse
    Clonality: Monoclonal
    Purity: Conjugated with fluorescein isothiocyanate (FITC) under optimum conditions, The conjugate is purified by size-exclusion chromatography.
    Format: Liquid at 1 mg/ml.
    Quantity: 0,1 mg
    Storage: Store in the dark at 2-8C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunocytochemistry (ICC), Flow cytometry (Flowcyt)
    Recommended dilution: 1-4 µg/ml (Flowcyt)
    Expected | apparent MW: 50 | 54 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Eisenia, Nicotiana tabacum, Paramecium, Saccharomyces cerevisiae, human, mouse, pig, turkey
    Predicted reactivity: Species of your interest not listed? Contact us
  • Application Examples
  • Immunocytochemistry using anti-tubulin alpha chain monoclonal antibodies, FITC conjugated

    HeLa cells stained with alpha tubulin monoclonal antibodies, conjugated with FITC.

  • Additional Information
  • Additional information: This antibody is recognizing a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin 1, UniProt: Q71U36
  • Background
  • Background:

    Tubulin alpha (TUA) together with beta tubulin is making up microtubules.

    The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs).  The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Antibody Protocols
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