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Tubulin alpha chain, Biotin conjugated (clone TU-01)
- Product Info
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Immunogen: Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization. UniProt: Q71U36 Sub class: IgG1 Host: Mouse Clonality: Monoclonal Purity: Conjugated with biotin-LC-NHS under optimum conditions and free of unconjugated biotin Format: Liquid at 1 mg/ml. Quantity: 0.1 mg Storage: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunohistochemistry (IHC) paraffin, Immunocytochemistry (ICC), Flow cytometry (Flowcyt), Western blot (WB) Recommended dilution: 1-2 μg/ml (WB) Expected | apparent MW: 50 | 54 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Eisenia, Nicotiana tabacum, Paramecium, Saccharomyces cerevisiae, human, mouse, pig, turkey Predicted reactivity: Species of your interest not listed? Contact us - Application Examples
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Samples: lysates of various cell lines under reducing (left to MW markers)and non-reducing conditions (right to MW markers).
Following SDS-PAGE, proteins were transferred to nitrocellulose membrane and incubated with 2 µg/ml of biotinylated mouse anti-alpha-tubulin monoclonal antibody followed by IRDye800-conjugated streptavidin. - Additional Information
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Additional information: This antibody is recognizing a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin 1, UniProt: Q71U36 - Background
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Background: Tubulin alpha (TUA) together with beta tubulin is making up microtubules.
The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. - Protocols
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Agrisera Western Blot protocol and video tutorials
Antibody Protocols - Reviews:
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Accessories
AS10 680 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C. reinhardii, E. gracilis, H. vulgare, O. sativa, Setaria italica, Zea mays
AS10 681 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana, Medicago sativa
AS20 4482 | Clonality: Monoclonal | Host: Mouse | Reactivity: Chlorella vulgaris, Nicotiana tabcum, Human, Protozoa
AS20 4484 | Clonality: Monoclonal | Host: Mouse | Reactivity: Human, mouse, pig