Tubulin alpha chain, Biotin conjugated (clone TU-01)

Product no: AS21 4624
AS21 4624 | Clonality: Monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana, Eisenia, Nicotiana tabacum, Paramecium, Saccharomyces cerevisiae, human, mouse, pig, turkey
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  • Product Info
  • Sub class: IgG1
    Immunogen: Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization. UniProt: Q71U36
    Host: Mouse
    Clonality: Monoclonal
    Purity: Conjugated with biotin-LC-NHS under optimum conditions and free of unconjugated biotin
    Format: Liquid at 1 mg/ml.
    Quantity: 0.1 mg
    Storage: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunohistochemistry (IHC) paraffin, Immunocytochemistry (ICC), Flow cytometry (Flowcyt), Western blot (WB)
    Recommended dilution: 1-2 μg/ml (WB)
    Expected | apparent MW: 50 | 54 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Eisenia, Nicotiana tabacum, Paramecium, Saccharomyces cerevisiae, human, mouse, pig, turkey
    Predicted reactivity: Species of your interest not listed? Contact us
  • Application Examples
  • Western blot using antitubulin alpha chain, monoclonal antibodies

    Samples: lysates of various cell lines under reducing (left to MW markers)and non-reducing conditions (right to MW markers).
    Following SDS-PAGE, proteins were transferred to nitrocellulose membrane and incubated with 2 µg/ml of biotinylated mouse anti-alpha-tubulin monoclonal antibody followed by IRDye800-conjugated streptavidin. 

  • Additional Information
  • Additional information: This antibody is recognizing a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin 1, UniProt: Q71U36
  • Background
  • Background:

    Tubulin alpha (TUA) together with beta tubulin is making up microtubules.

    The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs).  The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Antibody Protocols
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