Tubulin alpha chain, Biotin conjugated (clone TU-01)
AS21 4624 | Clonality: Monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana, Eisenia, Nicotiana tabacum, Paramecium, Saccharomyces cerevisiae, human, mouse, pig, turkey

Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization. UniProt: Q71U36
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG1
Purity
Conjugated with biotin-LC-NHS under optimum conditions and free of unconjugated biotin
Format
Liquid at 1 mg/ml.
Quantity
0.1 mg
Storage
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Immunohistochemistry (IHC) paraffin, Immunocytochemistry (ICC), Flow cytometry (Flowcyt), Western blot (WB)
Recommended dilution
1-2 μg/ml (WB)
Expected | apparent MW
50 | 54 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana, Eisenia, Nicotiana tabacum, Paramecium, Saccharomyces cerevisiae, human, mouse, pig, turkey
Predicted reactivity
Species of your interest not listed? Contact us
Application examples
Application examples

Samples: lysates of various cell lines under reducing (left to MW markers)and non-reducing conditions (right to MW markers).
Following SDS-PAGE, proteins were transferred to nitrocellulose membrane and incubated with 2 µg/ml of biotinylated mouse anti-alpha-tubulin monoclonal antibody followed by IRDye800-conjugated streptavidin.
Additional information
Additional information
This antibody is recognizing a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin 1, UniProt: Q71U36
Background
Background
Tubulin alpha (TUA) together with beta tubulin is making up microtubules.
Product citations
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