Tubulin alpha chain, Biotin conjugated (clone TU-01)

AS21 4624 | Clonality: Monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana, Eisenia, Nicotiana tabacum, Paramecium, Saccharomyces cerevisiae, human, mouse, pig, turkey

Tubulin alpha chain, Biotin conjugated (clone TU-01)

Product Information

Immunogen Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization. UniProt: Q71U36

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Conjugated with biotin-LC-NHS under optimum conditions and free of unconjugated biotin

Format Liquid at 1 mg/ml.

Quantity 0.1 mg

Storage Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunohistochemistry (IHC) paraffin, Immunocytochemistry (ICC), Flow cytometry (Flowcyt), Western blot (WB)

Recommended dilution 1-2 μg/ml (WB)

Expected | apparent MW 50 | 54 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Eisenia, Nicotiana tabacum, Paramecium, Saccharomyces cerevisiae, human, mouse, pig, turkey

Predicted reactivity Species of your interest not listed? Contact us

Application examples

Application examples Western blot using antitubulin alpha chain, monoclonal antibodies

Samples: lysates of various cell lines under reducing (left to MW markers)and non-reducing conditions (right to MW markers).
Following SDS-PAGE, proteins were transferred to nitrocellulose membrane and incubated with 2 µg/ml of biotinylated mouse anti-alpha-tubulin monoclonal antibody followed by IRDye800-conjugated streptavidin.

Additional information

Additional information This antibody is recognizing a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin 1, UniProt: Q71U36

Background

Tubulin alpha (TUA) together with beta tubulin is making up microtubules.

The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport.

Product citations

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