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Tubulin alpha chain, Biotin conjugated (clone TU-01)

AS21 4624 | Clonality: Monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana, Eisenia, Nicotiana tabacum, Paramecium, Saccharomyces cerevisiae, human, mouse, pig, turkey
Tubulin alpha chain, Biotin conjugated (clone TU-01) in the group Antibodies for Plant/Algal  / Loading Controls at Agrisera AB (Antibodies for research) (AS21 4624)

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Product Information

Immunogen Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization. UniProt: Q71U36
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity The purified antibody is conjugated with biotin-LC-NHS under optimum conditions and free of unconjugated biotin.
Format Liquid at 1 mg/ml
Quantity 0.1 mg
Storage Store at 2-8°C. Do not freeze. Do not use after the expiration date stamped on the label. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications Immunohistochemistry (IHC) paraffin, Immunocytochemistry (ICC), Flow cytometry (Flowcyt), Western blot (WB)
Recommended dilution 1-2 μg/ml (WB)
Expected | apparent MW 50 | 54 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Eisenia, Nicotiana tabacum, Paramecium, Saccharomyces cerevisiae, human, mouse, pig, turkey
Predicted reactivity Species of your interest not listed? Contact us

Application examples

Application examples Western blot using antitubulin alpha chain, monoclonal antibodies

Samples: lysates of various cell lines under reducing (left to MW markers)and non-reducing conditions (right to MW markers).
Following SDS-PAGE, proteins were transferred to nitrocellulose membrane and incubated with 2 µg/ml of biotinylated mouse anti-alpha-tubulin monoclonal antibody followed by IRDye800-conjugated streptavidin. 

Additional information

Additional information This antibody is recognizing a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin 1, UniProt: Q71U36

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Background

Background

Tubulin alpha (TUA) together with beta tubulin is making up microtubules.

The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs).  The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport.

Product citations

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