Tubulin alpha chain, Biotin conjugated (clone TU-01)
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Samples: lysates of various cell lines under reducing (left to MW markers)and non-reducing conditions (right to MW markers).
Following SDS-PAGE, proteins were transferred to nitrocellulose membrane and incubated with 2 µg/ml of biotinylated mouse anti-alpha-tubulin monoclonal antibody followed by IRDye800-conjugated streptavidin.
Tubulin alpha (TUA) together with beta tubulin is making up microtubules.