TYLCV V2 | Tomato yellow leaf curl virus coat protein V2

Detection of TYLCV V2 in tomato leaf and stem 28 and 49 days (dpi) after infection. a: western blot of V2 and OE33 (chloroplast protein used as an internal marker). b: western blot of V2 in leaf and stem tissues at 49 dpi fractioned into cytoplasmic/membrane (Cyt) and nuclear (N) components; cytoplasmic Hsp70 and nuclear histone H3 used as internal markers to assess the fraction purity. c: western blot of V2 in leaf and stem tissues of 49 dpi fractionated into insoluble debris and cell wall (P), 3000?g pellet (P3) and soluble protein (S3). d: western blot analysis of V2, distributed in linear 10-50% sucrose gradients from leaf and stem native protein extracts at 49 dpi; gradients were divided into 10 fractions, 1 (top) to 10 (bottom) and aliquots were subjected to SDS-PAGE.

Detection of TYLCV V2 in tomato leaf and stem 28 and 49 days (dpi) after infection. a: western blot of V2 and OE33 (chloroplast protein used as an internal marker). b: western blot of V2 in leaf and stem tissues at 49 dpi fractioned into cytoplasmic/membrane (Cyt) and nuclear (N) components; cytoplasmic Hsp70 and nuclear histone H3 used as internal markers to assess the fraction purity. c: western blot of V2 in leaf and stem tissues of 49 dpi fractionated into insoluble debris and cell wall (P), 3000?g pellet (P3) and soluble protein (S3). d: western blot analysis of V2, distributed in linear 10-50% sucrose gradients from leaf and stem native protein extracts at 49 dpi; gradients were divided into 10 fractions, 1 (top) to 10 (bottom) and aliquots were subjected to SDS-PAGE.

Detection of TYLCV V2 in tomato leaf and stem 28 and 49 days (dpi) after infection. a: western blot of V2 and OE33 (chloroplast protein used as an internal marker). b: western blot of V2 in leaf and stem tissues at 49 dpi fractioned into cytoplasmic/membrane (Cyt) and nuclear (N) components; cytoplasmic Hsp70 and nuclear histone H3 used as internal markers to assess the fraction purity. c: western blot of V2 in leaf and stem tissues of 49 dpi fractionated into insoluble debris and cell wall (P), 3000?g pellet (P3) and soluble protein (S3). d: western blot analysis of V2, distributed in linear 10-50% sucrose gradients from leaf and stem native protein extracts at 49 dpi; gradients were divided into 10 fractions, 1 (top) to 10 (bottom) and aliquots were subjected to SDS-PAGE.

Detection of TYLCV V2 in tomato leaf and stem 28 and 49 days (dpi) after infection. a: western blot of V2 and OE33 (chloroplast protein used as an internal marker). b: western blot of V2 in leaf and stem tissues at 49 dpi fractioned into cytoplasmic/membrane (Cyt) and nuclear (N) components; cytoplasmic Hsp70 and nuclear histone H3 used as internal markers to assess the fraction purity. c: western blot of V2 in leaf and stem tissues of 49 dpi fractionated into insoluble debris and cell wall (P), 3000?g pellet (P3) and soluble protein (S3). d: western blot analysis of V2, distributed in linear 10-50% sucrose gradients from leaf and stem native protein extracts at 49 dpi; gradients were divided into 10 fractions, 1 (top) to 10 (bottom) and aliquots were subjected to SDS-PAGE.

V2 forms complexes with TYLCV genomic DNA. Detection of TYLCV DNA-V2 complexes in vitro (a) and in planta (b). a: PCR detection of viral DNA following immuno-capture with V2 antibody of mixtures of V2 and infected plant DNA; V2/DNA: V2 incubated with untreated DNA, V2/DNA?+?S1: V2 incubated with DNA treated with S1 nuclease, V2/-DNA: V2 incubated without DNA. M - 100 bp DNA marker. b: Southern blot analysis of viral DNA-V2 complexes from infected tomato plants; DNA: DNA from infected plants, V2/DNA: DNA extracted from immunoprecipitated V2 complexes, V2/DNA?+?S1: DNA extracted from immunoprecipitated V2 complexes and treated with S1 nuclease.