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TYLCV V2 | Tomato yellow leaf curl virus coat protein V2

AS15 3059   | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:  Tomato yellow leaf curl virus coat protein V2

TYLCV V2 | Tomato yellow leaf curl virus coat protein V2 in the group Antibodies Plant/Algal  / Plant Pathogens / Viruses at Agrisera AB (Antibodies for research) (AS15 3059)
TYLCV V2 | Tomato yellow leaf curl virus coat protein V2



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Product Information

Immunogen Recombinant fragment of V2 protein as described in Gorovits et al. (2013).
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 150 ĩl
Reconstitution For reconstitution add 150 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 500-1 : 1000 (IL), 1 : 200 (WB)
Expected | apparent MW

13 kDa

Reactivity

Confirmed reactivity Tomato yellow leaf curl virus coat 13 kDa
Predicted reactivity Tomato yellow leaf curl virus coat 13 kDa
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Reactant: Solanum lycopersicum (Tomato)

Application: Western Blotting

Pudmed ID: 25940862

Journal: Sci Rep

Figure Number: 1A

Published Date: 2015-05-05

First Author: Moshe, A., Belausov, E., et al.

Impact Factor: 4.13

Open Publication

Detection of TYLCV V2 in tomato leaf and stem 28 and 49 days (dpi) after infection. a: western blot of V2 and OE33 (chloroplast protein used as an internal marker). b: western blot of V2 in leaf and stem tissues at 49 dpi fractioned into cytoplasmic/membrane (Cyt) and nuclear (N) components; cytoplasmic Hsp70 and nuclear histone H3 used as internal markers to assess the fraction purity. c: western blot of V2 in leaf and stem tissues of 49 dpi fractionated into insoluble debris and cell wall (P), 3000?g pellet (P3) and soluble protein (S3). d: western blot analysis of V2, distributed in linear 10-50% sucrose gradients from leaf and stem native protein extracts at 49 dpi; gradients were divided into 10 fractions, 1 (top) to 10 (bottom) and aliquots were subjected to SDS-PAGE.


Reactant: Solanum lycopersicum (Tomato)

Application: Western Blotting

Pudmed ID: 25940862

Journal: Sci Rep

Figure Number: 1B

Published Date: 2015-05-05

First Author: Moshe, A., Belausov, E., et al.

Impact Factor: 4.13

Open Publication

Detection of TYLCV V2 in tomato leaf and stem 28 and 49 days (dpi) after infection. a: western blot of V2 and OE33 (chloroplast protein used as an internal marker). b: western blot of V2 in leaf and stem tissues at 49 dpi fractioned into cytoplasmic/membrane (Cyt) and nuclear (N) components; cytoplasmic Hsp70 and nuclear histone H3 used as internal markers to assess the fraction purity. c: western blot of V2 in leaf and stem tissues of 49 dpi fractionated into insoluble debris and cell wall (P), 3000?g pellet (P3) and soluble protein (S3). d: western blot analysis of V2, distributed in linear 10-50% sucrose gradients from leaf and stem native protein extracts at 49 dpi; gradients were divided into 10 fractions, 1 (top) to 10 (bottom) and aliquots were subjected to SDS-PAGE.


Reactant: Solanum lycopersicum (Tomato)

Application: Western Blotting

Pudmed ID: 25940862

Journal: Sci Rep

Figure Number: 1C

Published Date: 2015-05-05

First Author: Moshe, A., Belausov, E., et al.

Impact Factor: 4.13

Open Publication

Detection of TYLCV V2 in tomato leaf and stem 28 and 49 days (dpi) after infection. a: western blot of V2 and OE33 (chloroplast protein used as an internal marker). b: western blot of V2 in leaf and stem tissues at 49 dpi fractioned into cytoplasmic/membrane (Cyt) and nuclear (N) components; cytoplasmic Hsp70 and nuclear histone H3 used as internal markers to assess the fraction purity. c: western blot of V2 in leaf and stem tissues of 49 dpi fractionated into insoluble debris and cell wall (P), 3000?g pellet (P3) and soluble protein (S3). d: western blot analysis of V2, distributed in linear 10-50% sucrose gradients from leaf and stem native protein extracts at 49 dpi; gradients were divided into 10 fractions, 1 (top) to 10 (bottom) and aliquots were subjected to SDS-PAGE.


Reactant: Solanum lycopersicum (Tomato)

Application: Western Blotting

Pudmed ID: 25940862

Journal: Sci Rep

Figure Number: 1D

Published Date: 2015-05-05

First Author: Moshe, A., Belausov, E., et al.

Impact Factor: 4.13

Open Publication

Detection of TYLCV V2 in tomato leaf and stem 28 and 49 days (dpi) after infection. a: western blot of V2 and OE33 (chloroplast protein used as an internal marker). b: western blot of V2 in leaf and stem tissues at 49 dpi fractioned into cytoplasmic/membrane (Cyt) and nuclear (N) components; cytoplasmic Hsp70 and nuclear histone H3 used as internal markers to assess the fraction purity. c: western blot of V2 in leaf and stem tissues of 49 dpi fractionated into insoluble debris and cell wall (P), 3000?g pellet (P3) and soluble protein (S3). d: western blot analysis of V2, distributed in linear 10-50% sucrose gradients from leaf and stem native protein extracts at 49 dpi; gradients were divided into 10 fractions, 1 (top) to 10 (bottom) and aliquots were subjected to SDS-PAGE.


Reactant: Solanum lycopersicum (Tomato)

Application: Western Blotting

Pudmed ID: 25940862

Journal: Sci Rep

Figure Number: 3A

Published Date: 2015-05-05

First Author: Moshe, A., Belausov, E., et al.

Impact Factor: 4.13

Open Publication

V2 forms complexes with TYLCV genomic DNA. Detection of TYLCV DNA-V2 complexes in vitro (a) and in planta (b). a: PCR detection of viral DNA following immuno-capture with V2 antibody of mixtures of V2 and infected plant DNA; V2/DNA: V2 incubated with untreated DNA, V2/DNA?+?S1: V2 incubated with DNA treated with S1 nuclease, V2/-DNA: V2 incubated without DNA. M - 100 bp DNA marker. b: Southern blot analysis of viral DNA-V2 complexes from infected tomato plants; DNA: DNA from infected plants, V2/DNA: DNA extracted from immunoprecipitated V2 complexes, V2/DNA?+?S1: DNA extracted from immunoprecipitated V2 complexes and treated with S1 nuclease.

Additional information

This antibody is detecting recombinant TYLCV V2 protein

Related products

Related products

AS15 3055 | Anti-TYLCV C1 | Tomato yellow leaf curl virus coat protein C1, rabbit antibodies
AS15 3056 | Anti-TYLCV C2 | Tomato yellow leaf curl virus coat protein C2, rabbit antibodies
AS15 3057 | Anti-TYLCV C3 | Tomato yellow leaf curl virus coat protein C3, rabbit antibodies
AS15 3058 | Anti-TYLCV C4 | Tomato yellow leaf curl virus coat protein C4, rabbit antibodies
AS15 3048 | Anti-TYLCV CP | Tomato yellow leaf curl virus coat protein 30 kDa, rabbit antibodies

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background

TYLCV CP (Tomato yellow leaf curl virus coat protein V2) accumulates in tomato leaves during infection. This protein was detected in the phloem of associated cells. TYLCV virus attacts tomato cultures worldwide and is transmitted by the white flye Bemisia tabaci.

Product citations

Selected references Moshe et al. (2015). The Tomato yellow leaf curl virus V2 protein forms aggregates depending on the cytoskeleton integrity and binds viral genomic DNA. Sci Rep. 2015 May 5;5:9967. doi: 10.1038/srep09967.

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