Name:
Phone:
E-mail:
Address:






UmuD | Protein UmuD

AS21 4546  | Clonality: Polyclonal |  Host: Rabbit  | Reactivity: Escherichia coli

UmuD | Protein UmuD  in the group Antibodies Other Species / Bacteria at Agrisera AB (Antibodies for research) (AS21 4546)



DATA SHEET IN PDF

Qty: 
331
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen Highly purified, full length, recombinant UmuD protein from E.coli, UniProt: P0AG11
Host

Rabbit

Clonality Polyclonal
Purity Serum. Contains 0.05 % sodium azide.
Format Liquid
Quantity 100 ĩl
Storage Store at -20°C and make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW 17 kDa (intact) 14 kDa (processed)

Reactivity

Confirmed reactivity Escherichia coli

Application examples

Application examples Western blot using anti-UmuD antibodies

Samples: Protein extract from E. coli  DE274 ( lexA51, recA730) without mitomycin C treatment (1), treated with mitomycin C (2) was separated on SDS-PAGE following a transfer to a membrane. Incubation with primary antibodies at 1: 3000, followed by washes and incubation with HRP conjugated anti-rabbit IgG antibodies. Reaction was visualized by chemiluminescence according to manufacture's recommendations.

Additional information

Related products

Background

Background The products of umuD , umuC , and recA genes (SOS genes) are required for mutagenesis induced by radiation or chemical agents. Transcription of these SOS genes is repressed by a repressor, LexA protein in uninduced cells. Exposure of cells to DNA-damaging agents activates RecA protein to promote proteolytic cleavage of LexA protein. Inactivation of LexA protein by the cleavage consequently derepresses the SOS genes, umuD, C and recA . UmuD protein is then auto-cleaved, which is promoted by RecA protein ssDNA in a ATP-dependent manner. The processed UmuD protein is the active form for mutagenesis and the UmuD-UmuC complex functions as an error-prone translesion DNA polymerase.

Product citations

Selected references Shinagawa et al. (1998). RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis. Proc Natl Acad Sci U S A. 1988 Mar;85(6):1806-10. doi: 10.1073/pnas.85.6.1806. PMID: 3126496; PMCID: PMC279868.
immunogen: Highly purified, full length, recombinant UmuD protein from E.coli, UniProt: P0AG11
Host:

Rabbit

Clonality: Polyclonal
Purity: Serum. Contains 0.05 % sodium azide.
Format: Liquid
Quantity: 100 ĩl
storage: Store at -20°C and make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
recommended dilution: 1 : 3000 (WB)
calculated | apparent molecular mass [kDa]: 17 kDa (intact) 14 kDa (processed)
Confirmed reactivity: Escherichia coli
Picture (footer): Western blot using anti-UmuD antibodies

Samples: Protein extract from E. coli  DE274 ( lexA51, recA730) without mitomycin C treatment (1), treated with mitomycin C (2) was separated on SDS-PAGE following a transfer to a membrane. Incubation with primary antibodies at 1: 3000, followed by washes and incubation with HRP conjugated anti-rabbit IgG antibodies. Reaction was visualized by chemiluminescence according to manufacture's recommendations.
background: The products of umuD , umuC , and recA genes (SOS genes) are required for mutagenesis induced by radiation or chemical agents. Transcription of these SOS genes is repressed by a repressor, LexA protein in uninduced cells. Exposure of cells to DNA-damaging agents activates RecA protein to promote proteolytic cleavage of LexA protein. Inactivation of LexA protein by the cleavage consequently derepresses the SOS genes, umuD, C and recA . UmuD protein is then auto-cleaved, which is promoted by RecA protein ssDNA in a ATP-dependent manner. The processed UmuD protein is the active form for mutagenesis and the UmuD-UmuC complex functions as an error-prone translesion DNA polymerase.
All references: Shinagawa et al. (1998). RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis. Proc Natl Acad Sci U S A. 1988 Mar;85(6):1806-10. doi: 10.1073/pnas.85.6.1806. PMID: 3126496; PMCID: PMC279868.

Related products: UmuD | Protein UmuD

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 26 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...
201 €
AS21 4543   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Escherichia coli
342 €
AS21 4544   | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Escherichi...
331 €