UmuD | Protein UmuD

Product no: AS21 4546

AS21 4546  | Clonality: Polyclonal |  Host: Rabbit  | Reactivity: Escherichia coli

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  • Product Info
  • Immunogen: Highly purified, full length, recombinant UmuD protein from E.coli, UniProt: P0AG11
    Host:

    Rabbit

    Clonality: Polyclonal
    Purity: Serum. Contains 0.05 % sodium azide.
    Format: Liquid
    Quantity: 100 l
    Storage: Store at -20C and make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 3000 (WB)
    Expected | apparent MW: 17 kDa (intact) 14 kDa (processed)
  • Reactivity
  • Confirmed reactivity: Escherichia coli
  • Application Examples
  • Western blot using anti-UmuD antibodies

    Samples: Protein extract from E. coli  DE274 ( lexA51, recA730) without mitomycin C treatment (1), treated with mitomycin C (2) was separated on SDS-PAGE following a transfer to a membrane. Incubation with primary antibodies at 1: 3000, followed by washes and incubation with HRP conjugated anti-rabbit IgG antibodies. Reaction was visualized by chemiluminescence according to manufacture's recommendations.
  • Background
  • Background: The products of umuD , umuC , and recA genes (SOS genes) are required for mutagenesis induced by radiation or chemical agents. Transcription of these SOS genes is repressed by a repressor, LexA protein in uninduced cells. Exposure of cells to DNA-damaging agents activates RecA protein to promote proteolytic cleavage of LexA protein. Inactivation of LexA protein by the cleavage consequently derepresses the SOS genes, umuD, C and recA . UmuD protein is then auto-cleaved, which is promoted by RecA protein ssDNA in a ATP-dependent manner. The processed UmuD protein is the active form for mutagenesis and the UmuD-UmuC complex functions as an error-prone translesion DNA polymerase.
  • Product Citations
  • Selected references: Shinagawa et al. (1998). RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis. Proc Natl Acad Sci U S A. 1988 Mar;85(6):1806-10. doi: 10.1073/pnas.85.6.1806. PMID: 3126496; PMCID: PMC279868.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials
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