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UmuD | Protein UmuD

AS21 4546  | Clonality: Polyclonal |  Host: Rabbit  | Reactivity: Escherichia coli

UmuD | Protein UmuD  in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS21 4546)

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Product Information

Immunogen Highly purified, full length, recombinant UmuD protein from E.coli, UniProt: P0AG11
Host

Rabbit

Clonality Polyclonal
Purity Serum with 0.05 % sodium azide
Format Liquid
Quantity 100 µl
Storage Store at -20°C and make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW 17 kDa (intact) 14 kDa (processed)

Reactivity

Confirmed reactivity Escherichia coli

Application examples

Application examples Western blot using anti-UmuD antibodies

Samples: Protein extract from E. coli  DE274 ( lexA51, recA730) without mitomycin C treatment (1), treated with mitomycin C (2) was separated on SDS-PAGE following a transfer to a membrane. Incubation with primary antibodies at 1: 3000, followed by washes and incubation with HRP conjugated anti-rabbit IgG antibodies. Reaction was visualized by chemiluminescence according to manufacture's recommendations.

Additional information

Related products

Background

Background The products of umuD , umuC , and recA genes (SOS genes) are required for mutagenesis induced by radiation or chemical agents. Transcription of these SOS genes is repressed by a repressor, LexA protein in uninduced cells. Exposure of cells to DNA-damaging agents activates RecA protein to promote proteolytic cleavage of LexA protein. Inactivation of LexA protein by the cleavage consequently derepresses the SOS genes, umuD, C and recA . UmuD protein is then auto-cleaved, which is promoted by RecA protein ssDNA in a ATP-dependent manner. The processed UmuD protein is the active form for mutagenesis and the UmuD-UmuC complex functions as an error-prone translesion DNA polymerase.

Product citations

Selected references Shinagawa et al. (1998). RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis. Proc Natl Acad Sci U S A. 1988 Mar;85(6):1806-10. doi: 10.1073/pnas.85.6.1806. PMID: 3126496; PMCID: PMC279868.

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