UVR8 | Ultraviolet-B receptor UVR8

Product no: AS22 4716

AS22 4716   | Clonality:  Polyclonal |  Host:  Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana UVR8, UniProt: Q9FN03, TAIR: At5g63860
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Antigen affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution, add 50 µl, of sterile or deionized water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 47.12  kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Brassica napus, Coffea arabica, Capsicum annuum, Glycine soja, Gossypium australe, Hordeum vulgare, Ipomoea triloba, Malus domestica, Nicotiana benthamiana, Nicotiana tabacum, Solanum lycopersicum, Solanum tuberosum, Oryza sativa, Phtheirospermum japonicum, Populus alba x Populus x berolinensis, Senna tora, Triticum aestivum, Triticum urartu, Turnera subulata, Zea mays
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-UVR8 antibodies
    Each lane contains 20 µg of Arabidopsis thaliana whole leaf extract of the following genetic backgrounds:
    1. Columbia ecotype (UVR8 wild type)
    2. uvr8-6 mutant
    3. uvr8-6/proUVR8:YFP-UVR8 (described in Bernula et al. 2017)

    20 µg/well of total protein extracted from liquid N2-frozen leaves of adult plants using hot extraction buffer containing 65 mM Tris-HCl, pH 7.8, 4 M urea, 5% (w/v) SDS, 100 mM dithiothreitol, 15% (v/v) glycerol, and 0.05% (w/v) bromophenol blue and denatured at 95°C for 5 min. Samples were separated at RT on 10% SDS-PAGE and blotted for 2 h to PVDF (pore size of 45 µm), using wet transfer in the cold. Blot was blocked with 5% milk 1h/RT with agitation. The blot was incubated in the primary antibody at a dilution of 1:1000 in TBS-T during overnight (16 h) at 4 °C with agitation. The antibody solution was decanted, and the blot was rinsed briefly, then washed 5 times for 5 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 Agrisera) diluted to 1:10 000 in TBS-T for 1 h/RT with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent: AS16 ECL-S-10 AgriseraSuperBright. Exposure time was 0.6 s (iBright, 3x3 binning).

    Courtesy of Dr. András Viczián,Institute of Plant Biology. Biological Research Center Szeged Hungary

  • Background
  • Background: UVR8 (Ultraviolet-B receptor UVR8)  is a signaling component that acts as UV-B photoreceptor and plays a key role in establishing UV-protective responses in plants. Upon UV-B irradiation, UVR8 undergoes an immediate switch from homodimer to monomer, accumulates in the nucleus, interacts with the photomorphogenic repressor COP1 and regulates the expression of the transcription factor HY5 by associating with chromatin (through histone H2B binding) in the HY5 promoter region. Involved in controlling aspects of leaf growth and morphogenesis in response to UV-B, is required for normal progression of endocycle and has a regulatory role in stomatal differentiation as well as is required for plant circadian clock response to photomorphogenic UV-B light. Promotes photosynthetic efficiency at elevated levels of UV-B. Plays a role in mediating the effects of UV-B radiation on pathogen resistance by controlling the expression of the sinapate biosynthetic pathway.
    Alternative names: RCC1 domain-containing protein UVR8,Protein UV-B RESISTANCE 8.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items