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V-ATPase, A | Vacuolar H+-ATPase subunit A (ammonium sulfate purified IgG)

AS09 467  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, M. crystallinum, N. tabacum

V-ATPase, A | Vacuolar H+-ATPase subunit A (ammonium sulfate purified IgG) in the group Antibodies Plant/Algal / Membrane Transport System / Vacuolar membrane at Agrisera AB (Antibodies for research) (AS09 467)
V-ATPase, A | Vacuolar H+-ATPase subunit A (ammonium sulfate purified IgG)



DATA SHEET IN PDF

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403 €
Buy 2 items of this product for 357.00 €/items
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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit A, O23654, At1g78900
Host Rabbit
Clonality Polyclonal
Purity Ammonium sulfate purified, total IgG.
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 8000 (ELISA), 1 : 2000 (WB)
Expected | apparent MW

68.8 | 70 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana tabacum
Predicted reactivity Chlamydomonas reinhardtii, Brassica napus, Cucumis sativus, Gossypium mexicanum, Hordeum vulgare, Oryza sativa, Ostreococcus lucimarinus, Phaseolus aureus, Populus balsamifera, Physcomitrium patens, Solanum lycopersicon, Triticum aestivum, Zea mays
Species of your interest not listed? Contact us
Not reactive in Thermotoga neapolitana

Application examples

Application examples

Application example

western blot detection using anti-V-ATPase antibodies

1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-V-ATPase subunit A antibodies (AS09 467, 1:2000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Additional information

Additional information
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube label.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protocol for isolation of plant vacuolar membranes can be found here.
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Related products

Background

Background

V-ATPase subunit A is a catalytic subunit of V1 complex of vacuolar ATPase. This enzyme (EC=3.6.3.14) is involved in acidification process of various compartements of eucaryotic cell. This protein is coded by VHA-A gene. Alternative names: Vacuolar proton pump subunit alpha, vacuolar H(+)-ATPase subunit A, V-ATPase 69 kDa subunit

Product citations

calculated | apparent molecular mass [kDa]:

68.8 | 70 kDa (Arabidopsis thaliana)
Clonality: Polyclonal
Format: Lyophilized
Host: Rabbit
immunogen:

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit A, O23654, At1g78900
Purity: Ammonium sulfate purified, total IgG.
Quantity: 100 ĩl
recommended dilution: 1 : 8000 (ELISA), 1 : 2000 (WB)
Reconstitution: For reconstitution add 100 µl of sterile water
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: ELISA (ELISA), Western blot (WB)
Confirmed reactivity: Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana tabacum
predicted reactivity: Chlamydomonas reinhardtii, Brassica napus, Cucumis sativus, Gossypium mexicanum, Hordeum vulgare, Oryza sativa, Ostreococcus lucimarinus, Phaseolus aureus, Populus balsamifera, Physcomitrium patens, Solanum lycopersicon, Triticum aestivum, Zea mays
Species of your interest not listed? Contact us
not reactive in: Thermotoga neapolitana
additional information:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube label.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protocol for isolation of plant vacuolar membranes can be found here.
additional information (application):
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Picture (footer):

Application example

western blot detection using anti-V-ATPase antibodies

1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-V-ATPase subunit A antibodies (AS09 467, 1:2000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

background:

V-ATPase subunit A is a catalytic subunit of V1 complex of vacuolar ATPase. This enzyme (EC=3.6.3.14) is involved in acidification process of various compartements of eucaryotic cell. This protein is coded by VHA-A gene. Alternative names: Vacuolar proton pump subunit alpha, vacuolar H(+)-ATPase subunit A, V-ATPase 69 kDa subunit

Related products: V-ATPase, A | Vacuolar H+-ATPase subunit A (ammonium sulfate purified IgG)

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