V-ATPase, a1 | vacuolar H+-ATPase subunit a isoform 1

Product no: AS14 2822

AS14 2822 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit A, UniProt: Q8RWZ7-1, TAIR:  AT2G28520

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW:

    93 kDa (Arabidopsis thaliana)

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Capsella rubella, Cucumis sativus, Erythranthe guttata , Glycine soja, Lupinus angustifolius, Morus notabilis, Phaseolus vulgaris , Vitis vinifera
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Aplication example

    western blot using anti-VHa1 antibodies

    10 μg of microsomal membranes were isolated from 6-days-old etiolated (dark-grown) Arabidopsis thaliana seedlings with Extraction buffer (450mM sucrose, 50mM HEPES pH7.5, 5mM MgCl2, 1mM DTT, 1x protease inhibitor). Proteins were denaturated in SDS sample buffer for 5 min at 95°C. Proteins were separated on a 10% SDS-PAGE and blotted 1h to a nitrocellulose membrane using tank transfer. Blots were blocked with 4% dry milk in TBS-T for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody (#AS14 2822) at a dilution of 1:1000 in blocking buffer for 16h with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera #AS09 602) diluted to 1:25 000 in blocking buffer for 2h at RT with agitation. The blot was washed as above and developed for 3 min with ECL according to the manufacturer's instructions peqlab AceGlow Kit & INTAS digital imager. Exposure time was 3 min.

    Courtesy of Fabian Fink, University Heidelberg, Germany
  • Additional Information
  • Additional information (application):

    Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

  • Background
  • Background: V-ATPase subunit VHA-a1 is a subunit of the membrane-integral Vo subunit. VHA-a1 target the V-ATPase enzyme to the trans-golgi network/earl endosome (TGN/EE) in Arabidopsis thaliana. This enzyme is involved in acidification process of various compartements of eucaryotic cells. The protein is coded by VHA-a1 gene AT2G28520. Alternative names: vacuolar H(+)-ATPase subunit a1, V-ATPase 93 kDa subunit.
  • Protocols
  • Method for isolation of plant vacuolar membranes

    method for vacuolar membrane isolation

    Courtesy of Dr. Masayoshi Maeshima

     

     

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AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

AS16 ECL-S-1L, 1L

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