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V-PPase | vacuolar H+-pyrophosphatase

AS12 1849 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C. sativus, D. bardawil, H. vulgare, N. tabacum, P. abies, V. vinifera

V-PPase | vacuolar H+-pyrophosphatase in the group Antibodies for Plant/Algal  / Membrane Transport System / Vacuolar membrane at Agrisera AB (Antibodies for research) (AS12 1849)

DATA SHEET IN PDF

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Data sheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-PPase,UniProt P31414, TAIR AT1G15690

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 8000 (ELISA), 1 : 100 (IL), 1 : 2000 (WB)
Expected | apparent MW

80.8 | 73 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Cucumis sativus, Dunaliella bardawil, Hordeum vulgare, Nicotiana tabacum, Picea abies, Vitis vinifera
Predicted reactivity Oryza sativa, Ricinus communis, Populus trichocarpa, Saccharum officinarum, Zea mays, Vigna radiata
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example


western blot using anti-VPP-ase antibodies


Different amounts (depending of the tissue) of membrane proteins from developing xylem tissue of Norway spruce were separated in a gradient (4-15 %) SDS-PAGE gel and blotted 30 min to nitrocellulose membrane using a standard semi-dry Trans-Blot ® Turbo ™ (Bio-Rad) system. Blots were blocked with 5% non-fat milk protein for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody V-PPase (Agrisera) at a dilution of 1: 5 000 overnight with agitation. The antibody solution was decanted and the blot was rinsed briefly, and then washed 3 times for 15 min in TBS-T at RT with agitation. Blot was incubated in the secondary antibody (anti-rabbit IgG horseradish peroxidase conjugate, from Agrisera, AS09 602) diluted to 1: 10 000 for 1 h at RT with agitation. The blot was washed as above and the presence of V-PPase detected with ECL according to the manufacturer's instructions. Exposure time was ~ 1 second.

Courtesy of Luis Alexis Jimenez Barboza

Additional information

Additional information Antibodies will detect target protein in 1 µg of a crude membrane preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, less than µg load per well should be sufficient.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Related products

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collection of antibodies to tonoplast proteins

AS09 492 | Anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma), rabbit antibodies

AS09 493 | Anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma), rabbit antibodies

AS09 494 | Anti-TIP2;1 (delta) | Delta-VM23, rabbit antibodies

AS09 510 | Anti-TIP2;1 | tonoplast intrinistic protein 2-1, rabbit antibodies

AS09 510 | Anti-TIP2;2 | tonoplast intrinistic protein 2-2, rabbit antibodies

AS09 511 | Anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal), rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

V-PPase (pyrophosphate-energized vacuolar membrane proton pump 1), EC=3.6.1.1, acts to establish proton gradient of often higher magnitude than H+-ATPase. Is involved in auxin transport and NaCl and drought tolerance.  Alternative names: pyrophosphate-energized inorganic pyrophosphatase 1, H(+)-PPase 1, vacuolar proton pyrophosphatase 1, vacuolar proton pyrophosphatase 3

Product citations

Selected references Prinsi et al. (2020). Root Proteomic Analysis of Two Grapevine Rootstock Genotypes Showing Different Susceptibility to Salt Stress. Int J Mol Sci. 2020 Feb 6;21(3). pii: E1076. doi: 10.3390/ijms21031076.
Patir-Nebioglu et al. (2019). Pyrophosphate modulates plant stress responses via SUMOylation. Elife. 2019 Feb 20;8. pii: e44213. doi: 10.7554/eLife.44213.
Migocka et al. (2015). Cucumber Metal Transport Protein CsMTP9 is a plasma membrane H+ -coupled antiporter involved in the Mn2+ and Cd2+ efflux from root cells. Plant J. 2015 Oct 20. doi: 10.1111/tpj.13056.
Migocka et al. (2014). Molecular and biochemical properties of two P 1B2 -ATPases, CsHMA3 and CsHMA4, from cucumber. Plant Cell Environ. 2014 Sep 11. doi: 10.1111/pce.12447.

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