VDAC1-5 | Voltage-dependent anion-selective channel protein 1-5, Biotin-conjugated (40 µg)

Name: VDAC1-5 | Voltage-dependent anion-selective channel protein 1-5, Biotin-conjugated (40 µg)
SKU: AS07 212B
Price: 464 EUR
Availability: InStock

AS07 212B | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, di and monocots | Cellular [compartment marker] of mitochondrial outer membrane

VDAC1-5 | Voltage-dependent anion-selective channel protein 1-5, Biotin-conjugated (40 µg) in the group Antibodies Plant/Algal / Mitochondria | Respiration at Agrisera AB (Antibodies for research) (AS07 212B)

VDAC1-5 | Voltage-dependent anion-selective channel protein 1-5, Biotin-conjugated (40 µg)

Product Information

Immunogen KLH-conjugated peptide conserved in all known higher plant VDAC proteins including Arabidopsis thaliana VDAC1 UniProt: Q9SRH5 , TAIR: AT3G01280, VDAC2 UniProt F4K3R8-1 , TAIR: AT5G67500, VDAC3 UniProt: Q9SMX3-1, TAIR: AT5G15090, VDAC4 UniProt: Q9FKM2-1, TAIR:AT5G57490, VDAC5 UniProt: Q9M2W6-1, TAIR: AT3G49920

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin.

Format Liquid

Quantity 40 µg

Storage Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Tested applications Blue-native PAGE (2D BN/SDS-PAGE), Immunolocalization (IL), Western blot (WB)

Recommended dilution 1 : 500 (IL), 1 : 2000, 2-30 µg protein/lane (WB)

Expected | apparent MW

29 kDa (for Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Beta vulgaris, Brassica oleracea var. botrytis, Oryza sativa, Papaver sp. pollen tubes (IL), Spinacia oleracea, Physcomitrium patens

Predicted reactivity

Arabidopsis alpina, Aundo donax, Brachypodium distachyon, Brassica campestris, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Citrus sinensis, Eutrema salsugineum, Glycine max, Glycine soja, Gossypium arboreum, Hoedum vulgare var. distichum, Jatropha curcas, Medicago truncatula, Mesembryanthemum crystallinum, Morus notabilis, Nicotiana tabacum, Phaseolus coccineus, Phaseolus vulgaris, Pisum sativum, Plantago major, Prunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays

Species of your interest not listed? Contact us

Not reactive in

Chlamydomonas reinhardtii, Glycine max, Zea mays, diatoms, Saccharomyces cerevisiae

Application examples

Additional information

Additional information Cellular [compartment marker] of mitochondrial outer membrane

Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 µg.

Immunolocalization method description and images are available here

Blue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010

Background

VDAC proteins are porin-type, beta-barrel diffusion pores. Prominently localized in the outer mitochondrial membrane and involved in metabolite exchange.

Product citations

immunogen:	KLH-conjugated peptide conserved in all known higher plant VDAC proteins including Arabidopsis thaliana VDAC1 UniProt: Q9SRH5 , TAIR: AT3G01280, VDAC2 UniProt F4K3R8-1 , TAIR: AT5G67500, VDAC3 UniProt: Q9SMX3-1, TAIR: AT5G15090, VDAC4 UniProt: Q9FKM2-1, TAIR:AT5G57490, VDAC5 UniProt: Q9M2W6-1, TAIR: AT3G49920
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin.
Format:	Liquid
Quantity:	40 µg
storage:	Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
tested applications:	Blue-native PAGE (2D BN/SDS-PAGE), Immunolocalization (IL), Western blot (WB)
recommended dilution:	1 : 500 (IL), 1 : 2000, 2-30 µg protein/lane (WB)
calculated \| apparent molecular mass [kDa]:	29 kDa (for Arabidopsis thaliana)
Confirmed reactivity:	Arabidopsis thaliana, Beta vulgaris, Brassica oleracea var. botrytis, Oryza sativa, Papaver sp. pollen tubes (IL), Spinacia oleracea, Physcomitrium patens
predicted reactivity:	Arabidopsis alpina, Aundo donax, Brachypodium distachyon, Brassica campestris, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Citrus sinensis, Eutrema salsugineum, Glycine max, Glycine soja, Gossypium arboreum, Hoedum vulgare var. distichum, Jatropha curcas, Medicago truncatula, Mesembryanthemum crystallinum, Morus notabilis, Nicotiana tabacum, Phaseolus coccineus, Phaseolus vulgaris, Pisum sativum, Plantago major, Prunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays Species of your interest not listed? Contact us
not reactive in:	Chlamydomonas reinhardtii, Glycine max, Zea mays, diatoms, Saccharomyces cerevisiae
additional information:	Cellular [compartment marker] of mitochondrial outer membrane
additional information (application):	Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 µg. Immunolocalization method description and images are available here Blue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010
background:	VDAC proteins are porin-type, beta-barrel diffusion pores. Prominently localized in the outer mitochondrial membrane and involved in metabolite exchange.