VDR1 | Violaxanthin de-epoxidase-related protein

Product no: AS12 2117

AS12 2117 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen:

    part of recombinant Arabidopsis thaliana VDR1 protein sequence UniProt: Q9SJ13, TAIR: At2g21860, excluding membrane spanning part

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 59 | 52,86 kDa (minus a transit peptide)
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Medicago truncatula, , Populus balsamifera, Ricinus communis, Vitis vinifera
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

    western blot using anti-VDR1 antibodies

    From 0.1-1.2 µg of chlorophyll from Arabidopsis thaliana total leaf extract, extracted with buffer A (330 mM sorbitol, 25 mM Tricine pH 7.8, 1 mM EDTA, 10 mM KCl, 0,15 % BSA, 4 mM Na ascorbat (L-ascorbic acid), 7 mM L-cysteine) were separated on 15 % SDS-PAGE gel and blotted 1h to PVDF. Blots were blocked with 10% skimmed non fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1 000 over night at 4ºC with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:10 000 in TTBS for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 1-3 min.

    Courtesy of Dr. Rikard Fristedt, UCLA, USA.



  • Background
  • Background:

    VDR1 (Violaxanthin de-epoxidase-related protein) is involved in a sterol biosythesis process and localized in a chloroplast.

  • Protocols


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