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VDR1 | Violaxanthin de-epoxidase-related protein

AS12 2117 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

VDR1 | Violaxanthin de-epoxidase-related protein in the group Plant/Algal Antibodies / Photosynthesis  / Light acclimation at Agrisera AB (Antibodies for research) (AS12 2117)

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product information
Background

VDR1 (Violaxanthin de-epoxidase-related protein) is involved in a sterol biosythesis process and localized in a chloroplast.

Immunogen

part of recombinant Arabidopsis thaliana VDR1 protein sequence Q9SJ13, At2g21860, excluding membrane spanning part

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS12 2604 | anti-VDE1 | VIOLAXANTHIN DEEPOXIDASE 1 rabbit antibody

other antibodies to GreenCut proteins

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

59 | 52.86 kDa (minus a transit peptide)

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Medicago truncatula, , Populus balsamifera, Ricinus communis, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references

to be added when available, antibody released in April 2013.


application example

western blot using anti-VDR1 antibodies

From 0.1-1.2 µg of chlorophyll from Arabidopsis thaliana total leaf extract, extracted with buffer A (330 mM sorbitol, 25 mM Tricine pH 7.8, 1 mM EDTA, 10 mM KCl, 0,15 % BSA, 4 mM Na ascorbat (L-ascorbic acid), 7 mM L-cysteine) were separated on 15 % SDS-PAGE gel and blotted 1h to PVDF. Blots were blocked with 10% skimmed non fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1 000 over night at 4ºC with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:10 000 in TTBS for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 1-3 min.

Courtesy of Dr. Rikard Fristedt, UCLA, USA.




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