VDR1 | Violaxanthin de-epoxidase-related protein

AS12 2117 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

VDR1 | Violaxanthin de-epoxidase-related protein

Product Information

Immunogen

part of recombinant Arabidopsis thaliana VDR1 protein sequence UniProt: Q9SJ13, TAIR: At2g21860, excluding membrane spanning part

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW 59 | 52,86 kDa (minus a transit peptide)

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Medicago truncatula, , Populus balsamifera, Ricinus communis, Vitis vinifera
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

application example

From 0.1-1.2 µg of chlorophyll from Arabidopsis thaliana total leaf extract, extracted with buffer A (330 mM sorbitol, 25 mM Tricine pH 7.8, 1 mM EDTA, 10 mM KCl, 0,15 % BSA, 4 mM Na ascorbat (L-ascorbic acid), 7 mM L-cysteine) were separated on 15 % SDS-PAGE gel and blotted 1h to PVDF. Blots were blocked with 10% skimmed non fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1 000 over night at 4ºC with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:10 000 in TTBS for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 1-3 min.

Courtesy of Dr. Rikard Fristedt, UCLA, USA.

Additional information

Background

VDR1 (Violaxanthin de-epoxidase-related protein) is involved in a sterol biosythesis process and localized in a chloroplast.

Product citations

calculated \| apparent molecular mass [kDa]:	59 \| 52,86 kDa (minus a transit peptide)
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	part of recombinant Arabidopsis thaliana VDR1 protein sequence UniProt: Q9SJ13, TAIR: At2g21860, excluding membrane spanning part
Purity:	Serum
Quantity:	50 µl
recommended dilution:	1 : 1000 (WB)
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Medicago truncatula, , Populus balsamifera, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	application example From 0.1-1.2 µg of chlorophyll from Arabidopsis thaliana total leaf extract, extracted with buffer A (330 mM sorbitol, 25 mM Tricine pH 7.8, 1 mM EDTA, 10 mM KCl, 0,15 % BSA, 4 mM Na ascorbat (L-ascorbic acid), 7 mM L-cysteine) were separated on 15 % SDS-PAGE gel and blotted 1h to PVDF. Blots were blocked with 10% skimmed non fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1 000 over night at 4ºC with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:10 000 in TTBS for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 1-3 min. Courtesy of Dr. Rikard Fristedt, UCLA, USA.
background:	VDR1 (Violaxanthin de-epoxidase-related protein) is involved in a sterol biosythesis process and localized in a chloroplast.