VSP | Vegetative storage protein 1
AS21 4522 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
DATA SHEET IN PDF| Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
Full length, purified recombinant His6-tagged VSP1 of Arabidopsis thaliana UniProt: O49195, TAIR: At5g24780
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG, purified on Protein A in PBS, 50 % glycerol, filter sterilized.
Format
Liquid
Quantity
200 ĩg
Storage
Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1: 1000 - 1: 2000 (WB)
Expected | apparent MW
28 | 27 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Arabidopsis thaliana maturing siliques were freshly extracted with 100 mm Tris-HCl, pH 6.8, 2% [w/v] SDS, 40% [v/v] glycerol, and 2% [v/v] 2-mercaptoethanol for SDS-PAGE and denatured at 95°C for 5 min. Sample was separated on 15 % SDS-PAGE and blotted at 15V overnight using wet transfer to PVDF membrane. Blot was thoroughly dried before blocking with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.
Blot below has been conducted with the same protocol, but ER bodies were induced in rosette leaves by treatment with MeJA.
Arabidopsis thaliana maturing siliques were freshly extracted with 100 mm Tris-HCl, pH 6.8, 2% [w/v] SDS, 40% [v/v] glycerol, and 2% [v/v] 2-mercaptoethanol for SDS-PAGE and denatured at 95°C for 5 min. Sample was separated on 15 % SDS-PAGE and blotted at 15V overnight using wet transfer to PVDF membrane. Blot was thoroughly dried before blocking with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.
Blot below has been conducted with the same protocol, but ER bodies were induced in rosette leaves by treatment with MeJA.
Samples:
Water treatment (1,2), treatment with 50 µM MeJA treatment (3,4); 50 µM MeJA plus 20 µl/L ethylene for 36h (5,6). Described in Matsushima et al. (2002).
Additional information
Reactivity of this antibody to VSP2 has not been determined, Sequence conservation of VSP1 and VSP2 is 86 % therefore, it is most likely that this antibody will also recognize VSP2,
Background
Background
VSP (Vegetative storage protein 1) may function as somatic storage protein during early seedling development. Expression of VSP1 is enhanced by wounding and protein is localized to vacuole.
Product citations
Selected references
Matsushima et al. (2002). An endoplasmic reticulum-derived structure that is induced under stress conditions in Arabidopsis.Plant Physiol. 2002 Dec;130(4):1807-14. doi: 10.1104/pp.009464.
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