IKP

Wheat gliadin

Product no: AS19 4331

AS19 4331 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: wheat gliadin

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  • Product Info
  • Immunogen: Wheat Gliadin (Native Protein)
    Host: Rabbit
    Clonality: Polyclonal
    Purity: >95%, Protein G purified to a total immunoglobulin G fraction.
    Format: Liquid
    Quantity: 50 g
    Storage: Store at -20C or -80C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western Blot (WB)
    Recommended dilution: 1 : 1000-1: 5000 (WB)
    Expected | apparent MW: 35 | 35 kDa
  • Reactivity
  • Confirmed reactivity: wheat gliadin
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    Western blot using anti-wheat gliadin antibodies

    2 and 10 µg of wheat flour were loaded/well and separated on 10 % SDS-PAGE  and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk for 2h/RT with agitation. Blot was incubated in the primary antibody at 2.µg/ml for 1h/RT with agitation in PBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4x in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in  for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent according to manufacture's instructions.
  • Additional Information
  • Additional information: Product contains a preservative: 0,03% Proclin 300 and 50% Glycerol, 10 mM PBS, pH 7,4
  • Background
  • Background: Wheat gliadin is a class of proteins being a main component of gluten fraction and present in wheat and other cereals.
  • Product Citations
  • Selected references: 38221900
  • Protocols
  • ELISA protocol

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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