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Wheat gliadin

AS19 4331 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: wheat gliadin

Wheat gliadin in the group Antibodies for Plant/Algal  / Food Proteins/Allergens at Agrisera AB (Antibodies for research) (AS19 4331)

DATA SHEET IN PDF

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Product Information

Immunogen Wheat Gliadin (Native Protein)
Host Rabbit
Clonality Polyclonal
Purity >95%, Protein G purified, total immunoglobulin G fraction
Format Liquid
Quantity 50 µg
Storage Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western Blot (WB)
Recommended dilution 1 : 1000-1: 5000 (WB)
Expected | apparent MW 35 | 35 kDa

Reactivity

Confirmed reactivity wheat gliadin
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

Western blot using anti-wheat gliadin antibodies

2 and 10 µg of wheat flour were loaded/well and separated on 10 % SDS-PAGE  and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk for 2h/RT with agitation. Blot was incubated in the primary antibody at 2.µg/ml for 1h/RT with agitation in PBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4x in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in  for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent according to manufacture's instructions.

Additional information

Additional information Product contains a preservative: 0.03% Proclin 300 and 50% Glycerol, 10 mM PBS, pH 7.4.

Related products

Background

Background Wheat gliadin is a class of proteins being a main component of gluten fraction and present in wheat and other cereals.

Product citations

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