Xyloglucan (monoclonal, clone LM25)

Name: Xyloglucan (monoclonal, clone LM25)
Brand: IKP
SKU: AS18 4205-1ml
Price: 246 EUR
Availability: InStock

AS18 4205-1ml | Clonality: Monoclonal | Host: Rat | Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom

Xyloglucan (monoclonal, clone LM25) in the group Antibodies Plant/Algal / Cell Wall / Dr. Knox collection, University of Leeds at Agrisera AB (Antibodies for research) (AS18 4205-1ml)

Product Information

Immunogen Neoglycoprotein, Xyloglucan

Host Rat

Clonality Monoclonal

Subclass/isotype IgM

Purity Cell culture supernatant.

Format Liquid

Quantity 1 ml

Storage Store at +4°C (short term) and at -20°C (long term).

Tested applications ELISA (ELISA), Immuniflourescence (IF)

Recommended dilution 1:10 (ELISA, IF)

Reactivity

Confirmed reactivity Higher plants, ferns and mosses

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Immunolocalization using monclonal anti-xyloglucan antibodies, LM25

Material: Cassava leaf, epidermal layer
Fixation: aldehyde, embedded in HM20 resin, thin sectioned
Blocking: 4% milk in PBS
Primary antibody: Anti-xyloglucan, LM25 (AS18 4205-1ml)
Secondary antibody (Agrisera): Donkey anti-Rat IgG (H&L), DyLight® 594 conjugated, AS12 2090 (red)
Counterstained with Calcofluor White (blue). Super-resolution images were acquired on a ZEISS Elyra7 in Lattice Structured Illumination Microscopy mode.

Courtesy Dr. Kirk Czymmek, Donald Danforth Plant Science Center, USA

Additional information

Additional information Contains 0,05% Sodium Azide

Background

Background The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Xyloglucan is a hemiceullose or polysaccharide that can be found in the primary cell wall.

Product citations

Selected references Pedersen et a. (2012). Versatile high resolution oligosaccharide microarrays for plant glycobiology and cell wall research. J Biol Chem. 2012 Nov 6;287(47):39429-38.doi: 10.1074/jbc.M112.396598.

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immunogen:	Neoglycoprotein, Xyloglucan
Sub class:	IgM
Host:	Rat
Clonality:	Monoclonal
Purity:	Cell culture supernatant.
Format:	Liquid
Quantity:	1 ml
storage:	Store at +4°C (short term) and at -20°C (long term).
tested applications:	ELISA (ELISA), Immuniflourescence (IF)
recommended dilution:	1:10 (ELISA, IF)
Confirmed reactivity:	Higher plants, ferns and mosses
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	Material: Cassava leaf, epidermal layer Fixation: aldehyde, embedded in HM20 resin, thin sectioned Blocking: 4% milk in PBS Primary antibody: Anti-xyloglucan, LM25 (AS18 4205-1ml) Secondary antibody (Agrisera): Donkey anti-Rat IgG (H&L), DyLight® 594 conjugated, AS12 2090 (red) Counterstained with Calcofluor White (blue). Super-resolution images were acquired on a ZEISS Elyra7 in Lattice Structured Illumination Microscopy mode. Courtesy Dr. Kirk Czymmek, Donald Danforth Plant Science Center, USA
additional information:	Contains 0,05% Sodium Azide
background:	The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Xyloglucan is a hemiceullose or polysaccharide that can be found in the primary cell wall.
All references:	Pedersen et a. (2012). Versatile high resolution oligosaccharide microarrays for plant glycobiology and cell wall research. J Biol Chem. 2012 Nov 6;287(47):39429-38.doi: 10.1074/jbc.M112.396598.