IKP

Xyloglucan (monoclonal, clone LM25)

Product no: AS18 4205-1ml
AS18 4205-1ml  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
246
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Delivery:  3-6 business days
  • Product Info
  • Immunogen: Neoglycoprotein, Xyloglucan
    Sub class: IgM
    Host: Rat
    Clonality: Monoclonal
    Purity: Cell culture supernatant.
    Format: Liquid
    Quantity: 1 ml
    Storage: Store at +4C (short term) and at -20C (long term).
    Tested applications: ELISA (ELISA), Immuniflourescence (IF)
    Recommended dilution: 1:10 (ELISA, IF)
  • Reactivity
  • Confirmed reactivity: Higher plants, ferns and mosses
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Immunolocalization using monclonal anti-xyloglucan antibodies, LM25

    Material: Cassava leaf, epidermal layer
    Fixation: aldehyde, embedded in HM20 resin, thin sectioned
    Blocking: 4% milk in PBS
    Primary antibody: Anti-xyloglucan, LM25 (AS18 4205-1ml)
    Secondary antibody (Agrisera): Donkey anti-Rat IgG (H&L), DyLight® 594 conjugated, AS12 2090 (red)
    Counterstained with Calcofluor White (blue). Super-resolution images were acquired on a ZEISS Elyra7 in Lattice Structured Illumination Microscopy mode.

    Courtesy Dr. Kirk Czymmek, Donald Danforth Plant Science Center, USA

  • Additional Information
  • Additional information: Contains 0,05% Sodium Azide
  • Background
  • Background: The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins.  Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Xyloglucan is a hemiceullose or polysaccharide that can be found in the primary cell wall. 
  • Product Citations
  • Selected references: Pedersen et a. (2012). Versatile high resolution oligosaccharide microarrays for plant glycobiology and cell wall research. J Biol Chem. 2012 Nov 6;287(47):39429-38.doi: 10.1074/jbc.M112.396598.
  • Protocols
  • Immunofluorsecence as described in: Developmental complexity of arabinan polysaccharides and their processing in plant cell walls Verhertbruggen Y, Marcus SE, Haeger A, Verhoef R, Schols HA, McCleary BV, McKee L, Gilbert HJ, Knox JP., The Plant Journal (2009) 59, 413–425.
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Accessories

AS18 4203-1ml | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
246 €
Add to cart
Info
AS18 4204-1ml  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
246 €
Add to cart
Info
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