Xyloglucan (monoclonal, clone LM25)

AS18 4205-1ml  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
Xyloglucan (monoclonal, clone LM25) in the group Antibodies Plant/Algal  / Cell Wall / Dr. Knox collection, University of Leeds at Agrisera AB (Antibodies for research) (AS18 4205-1ml)
Xyloglucan (monoclonal, clone LM25)


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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen Neoglycoprotein, Xyloglucan
Host Rat
Clonality Monoclonal
Subclass/isotype IgM
Purity Cell culture supernatant.
Format Liquid
Quantity 1 ml
Storage Store at +4°C (short term) and at -20°C (long term).
Tested applications ELISA (ELISA), Immuniflourescence (IF)
Recommended dilution 1:10 (ELISA, IF)


Confirmed reactivity Higher plants, ferns and mosses
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Immunolocalization using monclonal anti-xyloglucan antibodies, LM25

Material: Cassava leaf, epidermal layer
Fixation: aldehyde, embedded in HM20 resin, thin sectioned
Blocking: 4% milk in PBS
Primary antibody: Anti-xyloglucan, LM25 (AS18 4205-1ml)
Secondary antibody (Agrisera): Donkey anti-Rat IgG (H&L), DyLight® 594 conjugated, AS12 2090 (red)
Counterstained with Calcofluor White (blue). Super-resolution images were acquired on a ZEISS Elyra7 in Lattice Structured Illumination Microscopy mode.

Courtesy Dr. Kirk Czymmek, Donald Danforth Plant Science Center, USA

Additional information

Additional information Contains 0,05% Sodium Azide

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Background The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins.  Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Xyloglucan is a hemiceullose or polysaccharide that can be found in the primary cell wall. 

Product citations

Selected references Pedersen et a. (2012). Versatile high resolution oligosaccharide microarrays for plant glycobiology and cell wall research. J Biol Chem. 2012 Nov 6;287(47):39429-38.doi: 10.1074/jbc.M112.396598.

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