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Xyloglucan (monoclonal, clone LM15)

AS18 4203-1ml | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
Xyloglucan (monoclonal, clone LM15) in the group Antibodies Plant/Algal  / Cell Wall / Dr. Knox collection, University of Leeds at Agrisera AB (Antibodies for research) (AS18 4203-1ml)
Xyloglucan (monoclonal, clone LM15)



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Product Information

Immunogen Neoglycoprotein, Xyloglucan
Host Rat
Clonality Monoclonal
Subclass/isotype IgG2c
Purity Cell culture supernatant.
Format Liquid
Quantity 1 ml
Storage Store at +4°C (short term) and at -20°C (long term).
Tested applications ELISA (ELISA), Immuniflourescence (IF)
Recommended dilution 1:10 (ELISA, IF)

Reactivity

Confirmed reactivity  Higher plants, ferns and mosses
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Contains 0.05% Sodium Azide

Reacts with the XXXG motif of land plants xyloglucan.

This antibody is directed toward the nonreducing end, with the requirement for the second to last Glc to be substituted with an a-1,6-linked Xyl Ruprecht et al. (2017).

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Background

Background The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins.  Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Xyloglucan is a hemiceullose or polysaccharide that can be found in the primary cell wall. 

Product citations

Selected references Ruprecht et al. (2017). A Synthetic Glycan Microarray Enables Epitope Mapping of Plant Cell Wall Glycan-Directed Antibodies. Plant Physiol. 2017 Nov;175(3):1094-1104. doi: 10.1104/pp.17.00737.

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