For the last 30 years we have produced thousands of custom monoclonal and custom polyclonal antibodies for academic research worldwide as well as industry, diagnostics, and pharma. With our own animal facilities and immunological services in-house, we can offer antibody production for almost any research and scale needed. A list of chosen scientific references for antibodies produced at Agrisera can be found here.
Polyclonal and monoclonal antibodies
Both monoclonal and polyclonal antibodies can be used in variety of different applications including Western blot, ELISA, immunoprecipitation, immunofluorescence and immunohistochemistry and each have their own advantages and disadvantages which should be considered when deciding on choice of antibody.
Polyclonal antibodies are typically produced in rabbit, goat, hen or rat after activation of multiple B-cell clones. The polyclonal immune serum therefore contains a pool of antibodies with different chemical and physical properties to recognize multiple epitopes of the immunized antigen.
Advantages of recognizing multiple epitopes:
Monoclonal antibodies are typically produced from a single B-cell of immunized BalbC mice. By fusion of a B- and myeloma cell, a hybridoma cell line produces antibodies that recognize a single epitope with low cross reactivity.
Advantages of only recognizing one epitope of the antigen: