Choosing the most suitable protein sequence for generating an anti-peptide serum is the first and most crucial step in a successful immunization project.
Agrisera offers custom synthesized peptides produced by solid phase peptide synthesis (using Fmoc-Chemistry) including a terminal cysteine with the possibility for optional modifications (e.g. acetylation, biotinylation and fluorophore coupling). Peptides can be synthesized from immunograde purity (>70%, sufficient for generating or testing antibodies) to higher purity (>95%, required in enzymological and biological activity studies, among other applications). The purity is controlled by HPLC and mass spectrometric analysis (amino acid analysis and sequencing on request).
Standard design and synthesis service
Important note: Our standard service does not contain a detailed analysis of predicted cross-reactions to non-target proteins within the organism of interest. However, information provided on protein sequences (.txt-files or accession numbers) to which cross-reaction should be excluded will be considered. If possible, please specify additional information on regions that you would like to be targeted preferentially (or avoided), signal peptides, or any known target protein modifications (such as phosphorylation sites).
Coupling to carrier protein
KLH - The keyhole limpet hemocyanin protein is a copper-containing protein found in arthropods and mollusca. Its molecular size (>450 kDa) varies with its dissociation-states depending on the pH. Agrisera offers a polyclonal anti-KLH antibody.
BSA - The bovine serum albumin subunit of 67 kDa is a protein frequently used in immunoassays to block non-specific binding sites. It should not be used as carrier if future assays involve BSA (for instance as blocking agent). Agrisera offers a polyclonal anti-BSA antibody.
For additional information on possible custom adaptations of our standard peptide service (e.g. higher amounts of peptides, or request for individual assistance on complex peptide designs) please contact us at [email protected]