Goat anti-Rabbit IgG (H&L), ALP conjugated, min. cross-reactivity to bovine,goat,human, mouse,rat IgG
AS10 1086 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
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Product Information
Host
Goat
Clonality
Polyclonal
Purity
Affinity purified IgG
Format
Liquid
Quantity
1 mg
Storage
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution
1 : 500 -1 : 2000 (ELISA), 1 : 50 -1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500 -1 : 2000 (WB)
Reactivity
Application examples
Additional information
Additional information
This antibody reacts with: heavy chains on rabbit IgG light chains on all rabbit immunoglobulins based on immunoelectrophoresis.
Minimum cross-reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from bovine, goat, human, mouse or rat.
Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Background
Background
Goat anti-rabbit IgG (H&L) is an ALP (alkaline phosphatase) conjugated secondary antibody which binds all rabbit IgGs in immunological assays. Antibody is adsorbed againstbovine,goat,human,mouse,rat IgG and affinity purified using solid phase rabbit IgG.
Product citations
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