RPS12 | Ribosomal protein S12 (chloroplastic)

AS12 2114 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii

Product Information

Immunogen

Recombinant full length RPS12 of Chlamydomonas reinhardtii, P14149, expressed in E.coli

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 10 000 (WB)

Expected | apparent MW

14.6 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardtii, Zea mays

Predicted reactivity

Bacteria, Dunaliella salina, Cannabis sativa, Chlorella vulgaris, Nannochloropsis gaditana, Oltmannsiellopsis viridis (marine flagellate), Pinus sp., Physcomitrella patens, Prochlorococcus marinus, Scenedesmus obliquus, Spinacia oleracea, Synechocystis sp. PCC6803, Synechococcus elongatus PCC 7942, Volvox carteri (green alga)

Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application example

10 µg of total protein from Chlamydomonas reinhardtii extracted with standard lysis buffer (Tris-HCl pH 6.8 50mM, 10mM EDTA, 2% SDS, Sigma protease inhibitors) were separated on 15 % SDS-PAGE and blotted to nitrocellulose membrane using a wet transfer cell. Blot was blocked in TBS-T containing 5% non-fat dry milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 in TBS-T containing 5% non-fat dry milk for 1h at RT with agitation. The antibody solution was decanted and the blot was washed 3 times for 15 min in TBS-T containing 1% non-fat dry milk with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:0 000 in TBS-T containing 1% non-fat dry milk for 1h at RT with agitation. The blot was washed as above and developed for 1 min with ECL according to the manufacturers instructions. Exposure time was 30 seconds.

Courtesy of Dr. Silvia Ramundo, University of Geneva, Switzerland

Additional information

Additional information This product can be sold containing proclin if requested.

Related products

Plant and algal protein extraction buffer

Secondary antibodies

Background

RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy.

Product citations

Selected references Wang et al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 .
Zoschke et al. (2016). The PPR-SMR protein PPR53 enhances the stability and translation of specific chloroplast RNAs in maize. Plant J. 2016 Mar;85(5):594-606. doi: 10.1111/tpj.13093. Epub 2016 Feb 5.
Ramundo et al. (2013). Repression of Essential Chloroplast Genes Reveals New Signaling Pathways and Regulatory Feedback Loops in Chlamydomonas. The Plant Cell.