PAG1 | 20S Proteasome alpha subunit G1

Name: PAG1 | 20S Proteasome alpha subunit G1
SKU: AS19 4259
Price: 319 EUR
Availability: InStock

Product no: AS19 4259

AS19 4259 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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DATA SHEET IN PDF

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Product Info

Immunogen:	Recombinant, full length PAG1 of Arabidopsis thaliana, UniProt: O23715-1, TAIR: At2g27020 overexpressed in E.coli, purified from a gel piece
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 �l
Reconstitution:	For reconstitution add 50 �l, of sterile water
Storage:	Store lyophilized/reconstituted at -20�C (short tem, months) or at -80�C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 5000 (WB)
Expected \| apparent MW:	27,4 \| 26 kDa

Reactivity
Confirmed reactivity: Arabidopsis thaliana

Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Background

Background:

PAG1 (20S Proteasome alpha subunit G1) is a component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits.

Alternative name: 20S proteasome alpha subunit G-1, DiDi 17A-2a, Proteasome component 8, Proteasome subunit alpha type-7.

Product Citations

Selected references:

Book et al. (2010). Affinity purification of the Arabidopsis 26 S proteasome reveals a diverse array of plant proteolytic complexes. J Biol Chem. 2010 Aug 13;285(33):25554-69. doi: 10.1074/jbc.M110.136622.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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