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RecA (protein, positive control)

AS21 4542PProtein/positive control for Western blot
RecA (protein, positive control) in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS21 4542P)

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Product name, number (Agrisera, Sweden)

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Product Information

Purity Over 90 %, SDS-PAGE
Format Liquid, 50% glycerol, 20 mM Tris-HCl (pH 8), 1 mM EDTA, 150 mM KCl, 1 mM DTT
Quantity 100 µg
Storage Store at -20°C or -80°C for a longer period of time; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Expected | apparent MW 38 kDa

Reactivity

Application examples

Application examples E.coli RecA protein, positive control for Western blot

5 and 2 µg of highly purified RecA protein from Escherichia coli was separated on SDS-PAGE and stained by Coomasie.

Additional information

Additional information This product can be used in:
  • Studies on homologous recombination mechanism and SOS response
  • Screening using a probe from library by promotion of DNA hybridization
  • DNA visualization by electron microscopy due to nucleaofilament formation with DNA
RecA protein is full length, highly purified (over 90 %, SDS-PAGE) by several steps of chromatography. Provided at a concentration of 1 mg/ml estimated by BCA method. UniProt: P0A7G6

Related products

Related products AS21 4542 | Anti-RecA | LexA repressor, rabbit antibodies

Background

Background E. coli RecA protein is a very important enzyme for homologous recombination and recombinational repair. Its synthesis is induced by SOS response caused by DNA damage. RecA protein has multiple functions such as single stranded DNA dependent ATPase activity, DNA annealing activity, formation of D-loop and Holliday structure in homologous recombination reaction, and coprotease activities that promote self-cleavages of LexA repressor, lambda phage repressor and UmuD protein. RecA protein binds to single and double stranded DNA for nucleofilament formation. It carries out a central role in homologous recombination.

Product citations

Selected references Ishibashi, Oura S & Umemura (2017) Adsorption of DNA binding proteins to functionalized carbon nanotube surfaces with and without DNA wrapping. Eur Biophys J. 2017 Sep;46(6):541-547. doi: 10.1007/s00249-017-1200-3. Epub 2017 Feb 15. PMID: 28204854.
Oura et al. (2015) Biomolecular recognition ability of RecA proteins for DNA on single-walled carbon nanotubes. Colloids Surf B Biointerfaces. 2015 Feb 1;126:496-501. doi: 10.1016/j.colsurfb.2015.01.002. Epub 2015 Jan 10. PMID: 25612818.

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