RecA | Recombinase A

AS21 4542 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Escherichia coli
RecA | Recombinase A in the group Antibodies Other Species / Bacteria at Agrisera AB (Antibodies for research) (AS21 4542)


How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen Hihgly purified, full length (352 amino acids) RecA protein of E.coli, UniProt: P0A7G6
Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS. Contains 50% glycerol, filter sterilized.
Format Liquid
Quantity 50 ĩg
Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Indirect immunofluorescent (IF), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1: 600 (IP), 1: 1000 - 1: 3000 (WB)
Expected | apparent MW 38 | 37  kDa


Confirmed reactivity Escherichia coli

Application examples

Application examples Western blot using RecA antibodies

Samples of 2 µg of: Purified RecA protein, 8 ng (1), Crude extract of E.coli wild type (2), recA mutant (3), lexA mutant (SOS genes overexpressed) (4).  Following transfer to PVDF membrane, it was blocked with 5 % non-fat milk. The primary antibody was used at 1: 3000. Visualization was done using chemiluminescence.

Additional information

Additional information Antibody is provided at 1 mg/ml

Related products

Related products AS21 4541 | Anti-LexA | LexA repressor, rabbit antibodies


Background RecA (Recombinase A) plays critically important roles in homologous recombination, recombination repair and regulation of cellular responses to DNA damage (SOS response). RecA promotes auto-cleavage of LexA repressor by its coprotease activity after DNA damage, and induces many proteins related to DNA repair including RecA itself.

Product citations

Related products: RecA | Recombinase A

AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volum...
From 15 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
194 €
AS21 4541 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Escherichia coli
330 €