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RecA | Recombinase A

AS21 4542 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Escherichia coli
RecA | Recombinase A in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS21 4542)

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Product Information

Immunogen Hihgly purified, full length (352 amino acids) RecA protein of E.coli, UniProt: P0A7G6
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Liquid, in PBS with 50% glycerol, filter sterilized
Quantity 50 µg
Storage Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Indirect immunofluorescent (IF), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1: 600 (IP), 1: 1000 - 1: 3000 (WB)
Expected | apparent MW 38 | 37  kDa

Reactivity

Confirmed reactivity Escherichia coli

Application examples

Application examples Western blot using RecA antibodies

Samples of 2 µg of: Purified RecA protein, 8 ng (1), Crude extract of E.coli wild type (2), recA mutant (3), lexA mutant (SOS genes overexpressed) (4).  Following transfer to PVDF membrane, it was blocked with 5 % non-fat milk. The primary antibody was used at 1: 3000. Visualization was done using chemiluminescence.

Additional information

Additional information Antibody is provided at 1 mg/ml.

Related products

Related products AS21 4541 | Anti-LexA | LexA repressor, rabbit antibodies

Background

Background RecA (Recombinase A) plays critically important roles in homologous recombination, recombination repair and regulation of cellular responses to DNA damage (SOS response). RecA promotes auto-cleavage of LexA repressor by its coprotease activity after DNA damage, and induces many proteins related to DNA repair including RecA itself.

Product citations

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