RecA | Recombinase A
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Samples of 2 µg of: Purified RecA protein, 8 ng (1), Crude extract of E.coli wild type (2), recA mutant (3), lexA mutant (SOS genes overexpressed) (4). Following transfer to PVDF membrane, it was blocked with 5 % non-fat milk. The primary antibody was used at 1: 3000. Visualization was done using chemiluminescence.
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