UGPase | UDP-glucose pyrophosphorylase (cytoplasm marker, dicots)

Product no: AS23 4916

AS23 4916 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

This product is a replacement for AS05 086

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Buy 3 items of this product for 217.00 €/items
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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana UGP1, UniProt: A0A1I9LT02 and UGP2, UniProt: Q9M9P3 TAIR: AT3G03250 , AT5G17310
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Antigen affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl, of sterile or deionized water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 - 1: 5000 (WB)
    Expected | apparent MW: 51.7 | 52.3 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Hordeum vulgare, Zea mays
    Predicted reactivity: Arachis hypogaea, Brachypodium distachyon, Brassica napus Cannabis sativa, Capsicum annuum, Glycine max, Gossypium sp., Malus domesticam, Manihot esculenta, Medicago truncatula, Nicotiana tabacum, Pisum sativum, Populus sp., Ricinus communis, Saccharum sp., Solanum lycopresicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao

    Species of your interest not listed? Contact us

    For monocotyl species, use AS14 2813
    Not reactive in: Marchantia polymorpha
  • Application Examples
  • Western blot using anti-plant UGPase, cytoplasm marker

    10 µg/well of total protein extracted from Arabidopsis thaliana leaf, using PEB extraction buffer (Agrisera AS08 300) and denatured with Invitrogen LDS sample buffer (4X) at 70°C/5 min.  Samples were separated on Invitrogen NuPage Bis-Tris 4-12% SDS-PAGE  and blotted for 1 h to Invitrogen PVDF (pore size of  0.45 µm), using: wet transfer. Blot was blocked with 5% milk in TBS-T for: 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with TBS-T Blocking. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG ALP conjugated, AS09 607 lot 2302 ) diluted to 1: 5 000 in TBS-T Blocking for 0,5h/RT with agitation. The blot was washed as above and developed with AS19 BCIP-NBT-PLUS lot 08225181 for 1 min. As soon as the desired band is detectable, briefly wash the membrane in generous amounts of deionized water. Transfer the membrane to fresh deionized water and incubate for 2 minutes with agitation before placing the membrane on Whatman paper to dry.

    Courtesy Agrisera

  • Background
  • Background: UDP-glucose pyrophosphorylase (UGPase, UDPGP) E.C=2.7.7.9.  is a key enzyme of synthesis of sucrose, cellulose and other saccharides. There are two cytoplasmic isoforms of UGPase-A (which share 94 % identity on amino acid level) and one chloroplastic UGPase-B isoform in Arabidopsis thaliana which share ca. 10-11 % of identity (Kleczkowski et al. 2011).
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items

AS13 2640 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Agostis stoloniferacv. ‘Penncross’,Arabidopsis thaliana, Brassica sp., Cannabis sativa L., Cucumis sativus, Cynara cardunculus, Glycine max, Hordeum vulgare, Nicotiana tabacum, Phaseolus vulgaris, Phoenix dactylifera, Picrorhiza kurroa, Setaria italica, Solanum tuberosum, Triticum aestivum, Zea mays

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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
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