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His-tag | 6xHis, conjugated to DyLightŪ 650 (Clone AD1,1,10)

AS15 2933 | Clonality: monoclonal |  Host: Mouse | Reactivity: Polyhisitidine tagged proteins (N- or C-terminus label)
His-tag | 6xHis, conjugated to DyLightŪ 650 (Clone AD1,1,10) in the group Tag Antibodies / His (3x, 6x) at Agrisera AB (Antibodies for research) (AS15 2933)



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Product Information

Immunogen

Polyhistidine (HHHHHH) tagged polypeptide

Host Mouse
Clonality Monoclonal
Purity Immunogen affinity purified in 50 mM tris (pH 7.8), 0.9% NaCl, 0.05% sodium azide.
Format Liquid
Quantity 100 ĩg (1 mg/ml)
Storage Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Tested applications High throughput screening (HTS), Immunofluorescence (IF), Immunohistochemisty (IHC), Western blot (WB)
Recommended dilution 1 : 200-1 : 500 (IF), 1 : 1000 (WB)

Reactivity

Application examples

Additional information

Additional information Antibody is provided in: 100 mM Sodium Phosphate (pH 7,4), 100 mM Sodium Chloride, 2 mM Sodium Azide

Related products

Background

Background Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine tag and is directly conjugated to DyLight®650. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag. 

DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Product citations

immunogen:

Polyhistidine (HHHHHH) tagged polypeptide

Host: Mouse
Clonality: Monoclonal
Purity: Immunogen affinity purified in 50 mM tris (pH 7.8), 0.9% NaCl, 0.05% sodium azide.
Format: Liquid
Quantity: 100 ĩg (1 mg/ml)
storage: Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
tested applications: High throughput screening (HTS), Immunofluorescence (IF), Immunohistochemisty (IHC), Western blot (WB)
recommended dilution: 1 : 200-1 : 500 (IF), 1 : 1000 (WB)
additional information: Antibody is provided in: 100 mM Sodium Phosphate (pH 7,4), 100 mM Sodium Chloride, 2 mM Sodium Azide
background: Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine tag and is directly conjugated to DyLight®650. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag. 

DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

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