His-tag | 6xHis, conjugated to DyLight® 650 (Clone AD1,1,10)

Name: His-tag | 6xHis, conjugated to DyLight® 650 (Clone AD1,1,10)
SKU: AS15 2933
Price: 443 EUR
Availability: InStock

AS15 2933 | Clonality: monoclonal | Host: Mouse | Reactivity: Polyhisitidine tagged proteins (N- or C-terminus label)

His-tag | 6xHis, conjugated to DyLight® 650 (Clone AD1,1,10) in the group Tag Antibodies / His (3x, 6x) at Agrisera AB (Antibodies for research) (AS15 2933)

Product Information

Immunogen

Polyhistidine (HHHHHH) tagged polypeptide

Host Mouse

Clonality Monoclonal

Purity Immunogen affinity purified in 50 mM tris (pH 7.8), 0.9% NaCl, 0.05% sodium azide.

Format Liquid

Quantity 100 µg (1 mg/ml)

Storage Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.

Tested applications High throughput screening (HTS), Immunofluorescence (IF), Immunohistochemisty (IHC), Western blot (WB)

Recommended dilution 1 : 200-1 : 500 (IF), 1 : 1000 (WB)

Reactivity

Application examples

Additional information

Additional information Antibody is provided in: 100 mM Sodium Phosphate (pH 7,4), 100 mM Sodium Chloride, 2 mM Sodium Azide

Background

Background Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine tag and is directly conjugated to DyLight®650. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag.

DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Product citations

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immunogen:	Polyhistidine (HHHHHH) tagged polypeptide
Host:	Mouse
Clonality:	Monoclonal
Purity:	Immunogen affinity purified in 50 mM tris (pH 7.8), 0.9% NaCl, 0.05% sodium azide.
Format:	Liquid
Quantity:	100 µg (1 mg/ml)
storage:	Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Tested applications:	High throughput screening (HTS), Immunofluorescence (IF), Immunohistochemisty (IHC), Western blot (WB)
recommended dilution:	1 : 200-1 : 500 (IF), 1 : 1000 (WB)
additional information:	Antibody is provided in: 100 mM Sodium Phosphate (pH 7,4), 100 mM Sodium Chloride, 2 mM Sodium Azide
background:	Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine tag and is directly conjugated to DyLight®650. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.