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8-Hydroxyguanosine | DNA/RNA oxidative damage (clone 15A3)

AS10 708-100  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: hydroxy guanosine

8-Hydroxyguanosine | DNA/RNA oxidative damage (clone 15A3) in the group Antibodies for Human/Animal  / Human Proteins / Oxidative stress at Agrisera AB (Antibodies for research) (AS10 708-100)

DATA SHEET IN PDF

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Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

8-hydroxy-guanosine-BSA and – casein conjugates

Host Mouse
Clonality Monoclonal
Subclass/isotype IgG2A
Purity Protein G purified
Format Liquid
Quantity 100 µg
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunoaffinity chromatography (IAP), Immunohistochemistry on frozen tissue and paraffin-embedded (IHC-Fr-P)
Recommended dilution The optimal working dilution should be determined by the investigator.

Reactivity

Confirmed reactivity Recognizes markers of oxidative damage to DNA (8-hydroxy-2’-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine)
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information Protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 0.65 mg/ml.

Protocol for immunostaining using this antibody can be found here.

Related products

Related products

AS10 708-25 | Anti- hydroxy guanosine monoclonal antibodies, 25 µg

Secondary antibodies

Background

Background

Oxidative derivate of guanosine is called 8-Hydroxyguanosine (8OHdG) and is used as a popular biomarker of oxidative stress.

Product citations

Selected references Poborilova et al. (2015). DNA hypomethylation concomitant with the overproduction of ROS induced by naphthoquinone juglone on tobacco BY-2 suspension cells. Environmental and Experimental Botany, Volume 113, May 2015, Pages 28–39.
Haigh and Drew (2015). Cavitation during the protein misfolding cyclic amplification (PMCA) method - The trigger for de novo prion generation? Biochem Biophys Res Commun. 2015 Apr 17. pii: S0006-291X(15)00726-3. doi: 10.1016/j.bbrc.2015.04.048.

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