8-Hydroxyguanosine | DNA/RNA oxidative damage (25 µg)

AS10 708-25 | Clonality: monoclonal | Host: Mouse | Reactivity: 8-hydroxyguanosine

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product information
Background		Oxidative derivate of guanosine is called 8-Hydroxyguanosine (8OHdG) and is used as a popular biomarker of oxidative stress.
Immunogen		8-hydroxy-guanosine-BSA and – casein conjugates
Host		Mouse
Clonality		Monoclonal
Clone		IgG2A (clone 15A3)
Purity		Protein G purified
Format		Liquid
Quantity		25 µg
Reconstitution
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		ELISA (ELISA), Immunoaffinity chromatography (IAP), Immunohistochemistry on frozen tissue and paraffin-embedded (IHC-Fr-P)
Related products		AS10 708-100 \| hydroxy guanosine monoclonal antibody, 100 µg Secondary antibodies
Additional information		Protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 0.65 mg/ml.

application information
Recommended dilution		The optimal working dilution should be determined by the investigator.
Expected \| apparent MW
Confirmed reactivity		Recognizes markers of oxidative damage to DNA (8-hydroxy-2’-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine)
Predicted reactivity
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		Protocol for immunostaining using this antibody can be found here.
Selected references		Haigh and Drew (2015). Cavitation during the protein misfolding cyclic amplification (PMCA) method - The trigger for de novo prion generation? Biochem Biophys Res Commun. 2015 Apr 17. pii: S0006-291X(15)00726-3. doi: 10.1016/j.bbrc.2015.04.048. Poborilova et al. (2015). DNA hypomethylation concomitant with the overproduction of ROS induced by naphthoquinone juglone on tobacco BY-2 suspension cells. Environmental and Experimental Botany, Volume 113, May 2015, Pages 28–39.

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