8-Hydroxyguanosine | DNA/RNA oxidative damage (clone 15A3)
AS10 708-25 | Clonality: monoclonal | Host: Mouse | Reactivity: 8-hydroxyguanosine
DATA SHEET IN PDF| Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
8-hydroxy-guanosine-BSA and – casein conjugates
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG2A
Purity
Protein G purified
Format
Liquid
Quantity
25 µg
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Immunoaffinity chromatography (IAP), Immunohistochemistry on frozen tissue and paraffin-embedded (IHC-Fr-P)
Recommended dilution
The optimal working dilution should be determined by the investigator.
Reactivity
Confirmed reactivity
Recognizes markers of oxidative damage to DNA (8-hydroxy-2’-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
Protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 0.65 mg/ml.
Protocol for immunostaining using this antibody can be found here.
Background
Background
Oxidative derivate of guanosine is called 8-Hydroxyguanosine (8OHdG) and is used as a popular biomarker of oxidative stress.
Product citations
Selected references
Poborilova et al. (2015). DNA hypomethylation concomitant with the overproduction of ROS induced by naphthoquinone juglone on tobacco BY-2 suspension cells. Environmental and Experimental Botany, Volume 113, May 2015, Pages 28–39.
Haigh and Drew (2015). Cavitation during the protein misfolding cyclic amplification (PMCA) method - The trigger for de novo prion generation? Biochem Biophys Res Commun. 2015 Apr 17. pii: S0006-291X(15)00726-3. doi: 10.1016/j.bbrc.2015.04.048.
Haigh and Drew (2015). Cavitation during the protein misfolding cyclic amplification (PMCA) method - The trigger for de novo prion generation? Biochem Biophys Res Commun. 2015 Apr 17. pii: S0006-291X(15)00726-3. doi: 10.1016/j.bbrc.2015.04.048.
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