ABA | Abscisic acid (C1) (for immunolocalization)

AS09 446 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Eucalyptus globulus, Pinus radiata

Product Information

Immunogen

BSA-conjugated abscisic acid (C1) via C1 carboxyl group

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum in PBS, pH 7.4

Format Liquid containing 50% glycerol

Quantity 200 µg

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Immunofluorescence (IF), Immunolocalization (IL)

Recommended dilution The optimal working dilution should be determined by the investigator.

Reactivity

Confirmed reactivity Abscisic acid (C1) in Arabidopsis thaliana, Eucalyptus globulus, Petunia hybrida L., Pinus radiata

Predicted reactivity Abscisic acid (C1)

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Useful references for immunolocalization work:
DeWitte et al. (1999). Dynamics of cytokinins in apical shoot meristems of a day neutral tobacco during floral transition and flower formation. Plant Physiol. 119:111-121.
Zavala et al. (1983). Localisation of a phytohormone using immunocytochemistry. J.Cell Biol. 97:1235-1239.

The antibody will recognize either the ABA conjugated to glucose ester (ABA-GE) or the ABA precursor: abscisic acid aldehyde. ABA aldehyde is however not usually present in plant tissue similarly to ABA alcohol which is also reactive. The antibodies will predominantly recognize only ABA and its glucosylester.

Related products

Background

Abscisic acid (ABA) is a plant hormone involved in different physiological responses as stimulation of the closure of stomata (water stress brings about an increase in ABA synthesis), iInhibition of shoot growth, and many others. ABA shown to have both inhibitory as well as many promoting functions.

Product citations

Selected references Dinis et al. (2018). Kaolin modulates ABA and IAA dynamics and physiology of grapevine under Mediterranean summer stress. J Plant Physiol. 2018 Jan;220:181-192. doi: 10.1016/j.jplph.2017.11.007.
Kovaleva et al. (2017). ABA and IAA control microsporogenesis in Petunia hybrida L. Protoplasma. 2017 Nov 13. doi: 10.1007/s00709-017-1185-x.
Escandón et al. (2016). Integrated physiological and hormonal profile of heat-induced thermotolerance in Pinus radiata. Tree Physiol. 2016 Jan;36(1):63-77. doi: 10.1093/treephys/tpv127. Epub 2016 Jan 12.
Ondzighi-Assoume et al. (2016). Environmental Nitrate Stimulates Root Tip Abscisic Acid Accumulation via Release from Inactive Stores. Plant Cell. 2016 Feb 17. pii: TPC2015-00946-RA.
Jesus et al. (2015). Salicylic acid application modulates physiological and hormonal changes in Eucalyptus globulus under water deficit. Environ and Exp Botany, Volume 118, October 2015, Pages 56–66.
Lacuesta et al. (2013). Immunolocalization of IAA and ABA in roots and needles of radiata pine (Pinus radiata) during drought and rewatering. Tree Physiol. May;33(5):537-49.