ABA | Abscisic acid (C1) (for immunolocalization)

AS09 446 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Eucalyptus globulus, Pinus radiata

ABA | Abscisic acid (C1) (for immunolocalization)

Product Information

Immunogen

BSA-conjugated abscisic acid (C1) via C1 carboxyl group

Host Rabbit

Clonality Polyclonal

Purity Total IgG. Protein G purified in PBS with 50 % glycerol.

Format Liquid

Quantity 200 µg

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunofluorescence (IF), Immunolocalization (IL)

Recommended dilution The optimal working dilution should be determined by the investigator

Reactivity

Confirmed reactivity Abscisic acid (C1) in Arabidopsis thaliana, Eucalyptus globulus, Petunia hybrida L., Pinus radiata, Populus trichocarpa

Predicted reactivity Abscisic acid (C1)

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Reactant: Populus trichocarpa (Black cottonwood)

Application: Immunohistochemistry-immunofluorescence

Pudmed ID: 32192046

Journal: Int J Mol Sci

Figure Number: 7A,B,C,D,E,F

Published Date: 2020-03-17

First Author: Wojciechowska, N., Wilmowicz, E., et al.

Impact Factor: 5.542

Open Publication

Immunolocalization of ABA (green fluorescence) during senescence process of fine, absorptive roots. (A,B) Root control (RC); (C,D) The first stage of root senescence (RS1); (E,F) The second stage of root senescence (RS2). Autofluorescence (red) of the cell wall was registered in order to visualize the cell/organ shape. Abbreviations: R, rhizodermis; C, parenchyma cortex cells; Ct, cytoplasm; Ph, phloem, X, xylem. Scale bars = 50 µm.

Reactant: Populus trichocarpa (Black cottonwood)

Application: Immunohistochemistry-immunofluorescence

Pudmed ID: 32192046

Journal: Int J Mol Sci

Figure Number: 8A,B,C,D,E,F,G,H,I

Published Date: 2020-03-17

First Author: Wojciechowska, N., Wilmowicz, E., et al.

Impact Factor: 5.542

Open Publication

Immunodetection of ABA (green fluorescence) and red autofluorescence of chlorophyll during leaf senescence. (A–C) Leaves control (LC); (D–F) The first stage of leaf senescence (LS1); (G–I), The second stage of leaf senescence (LS2). Abbreviations: VB, vascular bundle; PM, palisade mesophyll; SM, spongy mesophyll; E – epidermis. Scale bars = 50 µm.

Additional information

Useful references for immunolocalization work:
DeWitte et al. (1999). Dynamics of cytokinins in apical shoot meristems of a day neutral tobacco during floral transition and flower formation. Plant Physiol. 119:111-121.
Zavala et al. (1983). Localisation of a phytohormone using immunocytochemistry. J.Cell Biol. 97:1235-1239.

The antibody will recognize either the ABA conjugated to glucose ester (ABA-GE) or the ABA precursor: abscisic acid aldehyde, ABA aldehyde is however not usually present in plant tissue similarly to ABA alcohol which is also reactive, The antibodies will predominantly recognize only ABA and its glucosylester

Related products

AS09 422 | Anti-ABA | abscisic acid (C1) (quantity 1mg), rabbit antibodies
AS09 447 | Anti-ABA | abscisic acid (C1) (quantity 5mg), rabbit antibodies
collection of antibodies to other plant hormones
Secondary antibodies

Background

Abscisic acid (ABA) is a plant hormone involved in different physiological responses as stimulation of the closure of stomata (water stress brings about an increase in ABA synthesis), iInhibition of shoot growth, and many others. ABA shown to have both inhibitory as well as many promoting functions.