ABA | Abscisic acid (C1) (for immunolocalization)

Name: ABA | Abscisic acid (C1) (for immunolocalization)
Brand: IKP
SKU: AS09 446
Price: 228 EUR
Availability: InStock

AS09 446 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Eucalyptus globulus, Pinus radiata

ABA | Abscisic acid (C1) (for immunolocalization) in the group Antibodies Plant/Algal / Hormones / Abscisic acid at Agrisera AB (Antibodies for research) (AS09 446)

ABA | Abscisic acid (C1) (for immunolocalization)

Product Information

Immunogen

BSA-conjugated abscisic acid (C1) via C1 carboxyl group

Host Rabbit

Clonality Polyclonal

Purity Total IgG. Protein G purified in PBS with 50 % glycerol.

Format Liquid

Quantity 200 µg

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunofluorescence (IF), Immunolocalization (IL)

Recommended dilution The optimal working dilution should be determined by the investigator

Reactivity

Confirmed reactivity Abscisic acid (C1) in Arabidopsis thaliana, Eucalyptus globulus, Petunia hybrida L., Pinus radiata, Populus trichocarpa

Predicted reactivity Abscisic acid (C1)

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Reactant: Populus trichocarpa (Black cottonwood)

Application: Immunohistochemistry-immunofluorescence

Pudmed ID: 32192046

Journal: Int J Mol Sci

Figure Number: 7A,B,C,D,E,F

Published Date: 2020-03-17

First Author: Wojciechowska, N., Wilmowicz, E., et al.

Impact Factor: 5.542

Open Publication

Immunolocalization of ABA (green fluorescence) during senescence process of fine, absorptive roots. (A,B) Root control (RC); (C,D) The first stage of root senescence (RS1); (E,F) The second stage of root senescence (RS2). Autofluorescence (red) of the cell wall was registered in order to visualize the cell/organ shape. Abbreviations: R, rhizodermis; C, parenchyma cortex cells; Ct, cytoplasm; Ph, phloem, X, xylem. Scale bars = 50 µm.

Reactant: Populus trichocarpa (Black cottonwood)

Application: Immunohistochemistry-immunofluorescence

Pudmed ID: 32192046

Journal: Int J Mol Sci

Figure Number: 8A,B,C,D,E,F,G,H,I

Published Date: 2020-03-17

First Author: Wojciechowska, N., Wilmowicz, E., et al.

Impact Factor: 5.542

Open Publication

Immunodetection of ABA (green fluorescence) and red autofluorescence of chlorophyll during leaf senescence. (A–C) Leaves control (LC); (D–F) The first stage of leaf senescence (LS1); (G–I), The second stage of leaf senescence (LS2). Abbreviations: VB, vascular bundle; PM, palisade mesophyll; SM, spongy mesophyll; E – epidermis. Scale bars = 50 µm.

Additional information

Additional information ABA | Abscisic acid (C1) (for immunolocalization)

The antibody will recognize either the ABA conjugated to glucose ester (ABA-GE) or the ABA precursor: abscisic acid aldehyde, ABA aldehyde is however not usually present in plant tissue similarly to ABA alcohol which is also reactive, The antibodies will predominantly recognize only ABA and its glucosylester

Background

Abscisic acid (ABA) is a plant hormone involved in different physiological responses as stimulation of the closure of stomata (water stress brings about an increase in ABA synthesis), iInhibition of shoot growth, and many others. ABA shown to have both inhibitory as well as many promoting functions.

Product citations

immunogen:	BSA-conjugated abscisic acid (C1) via C1 carboxyl group
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Total IgG. Protein G purified in PBS with 50 % glycerol.
Format:	Liquid
Quantity:	200 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Immunofluorescence (IF), Immunolocalization (IL)
recommended dilution:	The optimal working dilution should be determined by the investigator
Confirmed reactivity:	Abscisic acid (C1) in Arabidopsis thaliana, Eucalyptus globulus, Petunia hybrida L., Pinus radiata, Populus trichocarpa
predicted reactivity:	Abscisic acid (C1)
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
More images:	Reactant: Populus trichocarpa (Black cottonwood) Application: Immunohistochemistry-immunofluorescence Pudmed ID: 32192046 Journal: Int J Mol Sci Figure Number: 7A,B,C,D,E,F Published Date: 2020-03-17 First Author: Wojciechowska, N., Wilmowicz, E., et al. Impact Factor: 5.542 Open Publication Immunolocalization of ABA (green fluorescence) during senescence process of fine, absorptive roots. (A,B) Root control (RC); (C,D) The first stage of root senescence (RS1); (E,F) The second stage of root senescence (RS2). Autofluorescence (red) of the cell wall was registered in order to visualize the cell/organ shape. Abbreviations: R, rhizodermis; C, parenchyma cortex cells; Ct, cytoplasm; Ph, phloem, X, xylem. Scale bars = 50 µm. Reactant: Populus trichocarpa (Black cottonwood) Application: Immunohistochemistry-immunofluorescence Pudmed ID: 32192046 Journal: Int J Mol Sci Figure Number: 8A,B,C,D,E,F,G,H,I Published Date: 2020-03-17 First Author: Wojciechowska, N., Wilmowicz, E., et al. Impact Factor: 5.542 Open Publication Immunodetection of ABA (green fluorescence) and red autofluorescence of chlorophyll during leaf senescence. (A–C) Leaves control (LC); (D–F) The first stage of leaf senescence (LS1); (G–I), The second stage of leaf senescence (LS2). Abbreviations: VB, vascular bundle; PM, palisade mesophyll; SM, spongy mesophyll; E – epidermis. Scale bars = 50 µm.
additional information:	ABA \| Abscisic acid (C1) (for immunolocalization)
additional information (application):	The antibody will recognize either the ABA conjugated to glucose ester (ABA-GE) or the ABA precursor: abscisic acid aldehyde, ABA aldehyde is however not usually present in plant tissue similarly to ABA alcohol which is also reactive, The antibodies will predominantly recognize only ABA and its glucosylester
background:	Abscisic acid (ABA) is a plant hormone involved in different physiological responses as stimulation of the closure of stomata (water stress brings about an increase in ABA synthesis), iInhibition of shoot growth, and many others. ABA shown to have both inhibitory as well as many promoting functions.