IKP

ACT 2,8,11 | Actin 2, 8, 11 (clone mAb13a)

Product no: AS16 3140

AS16 3140  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Arabidopsis thaliana, Nicotiana benthamiana

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Delivery:  7-10 business days
  • Product Info
  • Immunogen: Full length recombinant Arabidopsis thaliana Actin-2 (ACT2) expressed in E.coli, Uniprot: Q96292
    Sub class: IgG1
    Host: Mouse
    Clonality: Monoclonal
    Purity: IgG1 immunoglobulin purified on Protein G in 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) sodium azide.
    Format: Liquid
    Quantity: 100 g
    Storage: Store at -20C,Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1-2 ug/ml (WB)
    Expected | apparent MW:

    45 | 45 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Nicotiana tabacum
    Predicted reactivity: Actinidia deliciosa, Brachypodium sylvaticum, Brassica napus, Brassica oleracea, Cucumis sativus, Dionea muscipula, Euphorbia lathyris, Ficus microcarpa, Gentlisea aurea, Gossypium sp., Hevea brasiliensis, Medicago sativa, Medicago truncatula, Oryza sativa subsp. japonica, Oxytropis ochrocephala, Panax notoginseng, Populus trichocarpa, Phaseolus vulgaris, Prunus avium, Striga asiatica, Theobrom cacao, Torenia fournieri, Trifolium pratense, Vaccinium ashei, Ziziphus jujuba
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information:

    This antibody is a total IgG fraction, purified on a protein G column. It recognizes actin isoforms ACT2, 8, and 11.

    Additional information (application): Exact working dilution needs to be determined by end user
  • Background
  • Background: Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
  • Product Citations
  • Selected references: Kandasamy, M.K., et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell May;24(5):2041-57. doi: 10.1105/tpc.111.095281.
    Kandasamy, M.K., et al. (2001). One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell. Jul;13(7):1541-54.
    Kandasamy, M.K., et al. (1999). The late pollen-specific actins in angiosperms. Plant Journal. Jun;18(6):681-9.
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Accessories

AS16 3139  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Arabidopsis thaliana

387 €
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AS13 2640 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Agostis stoloniferacv. ‘Penncross’,Arabidopsis thaliana, Brassica sp., Cannabis sativa L., Cucumis sativus, Cynara cardunculus, Glycine max, Hordeum vulgare, Nicotiana tabacum, Phaseolus vulgaris, Phoenix dactylifera, Picrorhiza kurroa, Setaria italica, Solanum tuberosum, Triticum aestivum, Zea mays

319 €
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