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ACT 2,8,11 | Actin 2, 8, 11 (clone mAb13a)

AS16 3140  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Arabidopsis thaliana, Nicotiana benthamiana

ACT 2,8,11 | Actin 2, 8, 11 (clone mAb13a) in the group Antibodies for Plant/Algal  / Developmental Biology / Cytoskeleton at Agrisera AB (Antibodies for research) (AS16 3140)

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Product Information

Immunogen Full length recombinant Arabidopsis thaliana Actin-2 (ACT2) expressed in E.coli, Uniprot: Q96292
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Format Liquid
Quantity 100 µg
Storage Store at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1-2 ug/ml (WB)
Expected | apparent MW

45 | 45 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Nicotiana tabacum
Predicted reactivity Actinidia deliciosa, Brachypodium sylvaticum, Brassica napus, Brassica oleracea, Cucumis sativus, Dionea muscipula, Euphorbia lathyris, Ficus microcarpa, Gentlisea aurea, Gossypium sp., Hevea brasiliensis, Medicago sativa, Medicago truncatula, Oryza sativa subsp. japonica, Oxytropis ochrocephala, Panax notoginseng, Populus trichocarpa, Phaseolus vulgaris, Prunus avium, Striga asiatica, Theobrom cacao, Torenia fournieri, Trifolium pratense, Vaccinium ashei, Ziziphus jujuba
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

This antibody is a total IgG fraction, purified on a protein G column. It recognizes actin isoforms ACT2, 8, and 11.

Exact working dilution needs to be determined by end user.

Related products

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AS13 2640 | Anti-ACT | actin, rabbit antibodies
AS10 681
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AS10 680 | Anti-tubulin alpha chain, rabbit antibodies

AS10 702 | Anti-Actin-11, mouse monoclonal antibodies
AS16 3141 | Anti-Actin-8, mouse monoclonal antibodies
AS16 3139 | Anti-Actin-1, mouse monoclonal antibodkies

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.

Product citations

Selected references Kandasamy, M.K., et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell May;24(5):2041-57. doi: 10.1105/tpc.111.095281.
Kandasamy, M.K., et al. (2001). One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell. Jul;13(7):1541-54.
Kandasamy, M.K., et al. (1999). The late pollen-specific actins in angiosperms. Plant Journal. Jun;18(6):681-9.

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