ACT 2,8,11 | Actin 2, 8, 11

356 €

AS16 3140  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Arabidopsis thaliana, Nicotiana benthamiana


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Item No:
AS16 3140

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product information
Background Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
Immunogen Full length recombinant Arabidopsis thaliana Actin-2 (ACT2) expressed in E.coli, Uniprot: Q96292
Host Mouse
Clonality Monoclonal
Clone IgG1, clone mAb13a
Format Liquid
Quantity 100 ĩg
Storage Store at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications Immunohistochemistry (IHC), Western blot (WB)
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AS10 702 | Actin-11, mouse monoclonal antibody
AS16 3141 | Actin-8, mouse monoclonal antibody
AS16 3139 | Actin-1, mouse monoclonal antibody

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

This antibody is a total IgG fraction, purified on a protein G column. It recognizes actin isoforms ACT2, 8, and 11.

application information
Recommended dilution 1-2 ug/ml (WB)
Expected | apparent MW

45 | 45 kDa

Confirmed reactivity Arabidopsis thaliana, Nicotiana tabacum
Predicted reactivity Actinidia deliciosa, Brachypodium sylvaticum, Brassica napus, Brassica oleracea, Cucumis sativus, Dionea muscipula, Euphorbia lathyris, Ficus microcarpa, Gentlisea aurea, Gossypium sp., Hevea brasiliensis, Medicago sativa, Medicago truncatula, Oryza sativa subsp. japonica, Oxytropis ochrocephala, Panax notoginseng, Populus trichocarpa, Phaseolus vulgaris, Prunus avium, Striga asiatica, Theobrom cacao, Torenia fournieri, Trifolium pratense, Vaccinium ashei, Ziziphus jujuba
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information Exact working dilution needs to be determined by end user.
Selected references Kandasamy, M.K., et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell May;24(5):2041-57. doi: 10.1105/tpc.111.095281.
Kandasamy, M.K., et al. (2001). One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell. Jul;13(7):1541-54.
Kandasamy, M.K., et al. (1999). The late pollen-specific actins in angiosperms. Plant Journal. Jun;18(6):681-9.

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