showForm anti-AKINB1 | SNF1-related protein kinase regulatory subunit beta-1 antibodies

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AKING1 | SNF1-related protein kinase regulatory subunit gamma 1

AS09 613 | Clonality:Polyclonal | Host:Rabbit | Reactivity: Arabidopsis thaliana

AKING1 | SNF1-related protein kinase regulatory subunit gamma 1 in the group Antibodies for Plant/Algal  / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS09 613)

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Product Information

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana AKING1UniProt: Q8LBB2, TAIR: At3g48530

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

48 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity

Arabidopsis thaliana

Not reactive in

Chlamydomonas reinhardtii, Zea mays, Vitis vinifera

Application examples

Application examples

Application example

western blot using anti-KIN gamma antibodies

10 ug of Arabidopsis thaliana Columbia WT and KIN gamma KO seedlings were grinded in MOPS loading buffer and heated for 5 min at 95°C. Samples were separated on 10 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly
twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody, diluted to 1:4 000 in for 1h at RT with agitation.
The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 15 seconds.

Courtesy of Dr. Matthew Ramon

Additional information

Related products

Related products

AS09 460 | Anti-AKINB1 | SNF1-related protein kinase regulatory subunit beta-1, rabbit antibodies
AS09 462 | Anti-AKINB2 | SNF1-related protein kinase regulatory subunit beta-2, rabbit antibodies
AS15 2859 | Anti-AKIN ß3 | SNF1-related protein kinase regulatory subunit beta-3, rabbit antibodies
AS09 463 | Anti-AKIN beta gamma, rabbit antibodies
AS10 919 | Anti-AKIN10  SNF1-related protein kinase catalytic subunit alpha KIN10, rabbit antibodies
AS10 920 | Anti-AKIN11 | SNF1-related protein kinase catalytic subunit alpha KIN11, rabbit antiboddies

collection of antibodies to proteins involved in signal transduction

Plant protein extraction buffer

Secondary antibodies

Background

Background

AKING1 is a member of SnRK1/SNF1/AMPK family in plants. It is a regulatory subunit of the putative trimeric SNF1-related protein kinase (SnRK) comples which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.

Product citations

Selected references Emanuelle et al. (2015). SnRK1 from Arabidopsis thaliana is an atypical AMPK. Plant J. 2015 Mar 3. doi: 10.1111/tpj.12813.
Ramon et al. (2013). The hybrid Four-CBS-Domain KINβγ-subunit functions as the canonical γsubunit of the plant energy sensor SnRK1. Plant j. April 1.

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