V-ATPase, subunit 36 kDa (Avena sativa) (clone 7A3)

Product no: AS17 4163

AS17 4163 | Clonality: Monoclonal |  Host: Mouse | Reactivity: Avevena sativa

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  • Product Info
  • Immunogen: V-ATPase complex from Avena sativa purified by gel filtration Ward and Sze 1992
    Sub class: IgG
    Host: Mouse
    Clonality: Monoclonal, clone 7A3
    Purity: Cell culture supernatant.
    Format: Liquid
    Quantity: 1 ml
    Storage: Store at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications:

    Immunoprecipitation (IP), Western blot (WB)

    Recommended dilution: 1 : 50 - 1: 100 (WB)
    Expected | apparent MW: 36 kDa
  • Reactivity
  • Confirmed reactivity: Avena sativa
    Predicted reactivity:
    Avena sativa
    Not reactive in: other monocots
  • Background
  • Background: V-ATPase subunit A is a catalytic subunit of V1 complex of vacuolar ATPase. This enzyme (EC=3.6.3.14) is involved in acidification process of various compartements of eucaryotic cell.  This protein is coded by VHA-A gene. Alternative names: Vacuolar proton pump subunit alpha, vacuolar H(+)-ATPase subunit A, V-ATPase 69 kDa subunit
  • Product Citations
  • Selected references: Li and Sze (1999). A 100 kDa polypeptide associates with the V0 membrane sector but not with the active oat vacuolar H(+)-ATPase, suggesting a role in assembly. Plant J. 1999 Jan;17(1):19-30.
    Li and Sze (1999). A 100 kDa polypeptide associates with the V0 membrane sector but not with the active oat vacuolar H(+)-ATPase, suggesting a role in assembly. Plant J. 1999 Jan;17(1):19-30.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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