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V-ATPase, subunit 36 kDa (Avena sativa) (clone 7A3)

AS17 4163 | Clonality: Monoclonal |  Host: Mouse | Reactivity: Avevena sativa

V-ATPase, subunit 36 kDa (Avena sativa) (clone 7A3) in the group Antibodies for Plant/Algal  / Membrane Transport System / Vacuolar membrane at Agrisera AB (Antibodies for research) (AS17 4163)

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Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen V-ATPase complex from Avena sativa purified by gel filtration Ward and Sze 1992
Host Mouse
Clonality Monoclonal, clone 7A3
Subclass/isotype IgG
Format Liquid, culture supernatant
Quantity 1 ml
Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications

Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 1 : 50 - 1: 100 (WB)
Expected | apparent MW 36 kDa

Reactivity

Confirmed reactivity Avena sativa
Predicted reactivity
Avena sativa
Not reactive in other monocots

Application examples

Additional information

Related products

Background

Background V-ATPase subunit A is a catalytic subunit of V1 complex of vacuolar ATPase. This enzyme (EC=3.6.3.14) is involved in acidification process of various compartements of eucaryotic cell.  This protein is coded by VHA-A gene. Alternative names: Vacuolar proton pump subunit alpha, vacuolar H(+)-ATPase subunit A, V-ATPase 69 kDa subunit

Product citations

Selected references Li and Sze (1999). A 100 kDa polypeptide associates with the V0 membrane sector but not with the active oat vacuolar H(+)-ATPase, suggesting a role in assembly. Plant J. 1999 Jan;17(1):19-30.
Li and Sze (1999). A 100 kDa polypeptide associates with the V0 membrane sector but not with the active oat vacuolar H(+)-ATPase, suggesting a role in assembly. Plant J. 1999 Jan;17(1):19-30.

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