V-ATPase, subunit 36 kDa (Avena sativa) (clone 7A3)
AS17 4163 | Clonality: Monoclonal | Host: Mouse | Reactivity: Avevena sativa
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Immunogen V-ATPase complex from Avena sativa purified by gel filtration Ward and Sze 1992
Clonality Monoclonal, clone 7A3
Format Liquid, culture supernatant
Quantity 1 ml
Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1 : 50 - 1: 100 (WB)
Expected | apparent MW 36 kDa
Confirmed reactivity Avena sativa
Not reactive in other monocots
Background V-ATPase subunit A is a catalytic subunit of V1 complex of vacuolar ATPase. This enzyme (EC=18.104.22.168) is involved in acidification process of various compartements of eucaryotic cell. This protein is coded by VHA-A gene. Alternative names: Vacuolar proton pump subunit alpha, vacuolar H(+)-ATPase subunit A, V-ATPase 69 kDa subunit
Selected references Li and Sze (1999). A 100 kDa polypeptide associates with the V0 membrane sector but not with the active oat vacuolar H(+)-ATPase, suggesting a role in assembly. Plant J. 1999 Jan;17(1):19-30.
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