V-ATPase, subunit 36 kDa (Avena sativa) (clone 7A3)

Name: V-ATPase, subunit 36 kDa (Avena sativa) (clone 7A3)
SKU: AS17 4163
Price: 302 EUR
Availability: InStock

AS17 4163 | Clonality: Monoclonal | Host: Mouse | Reactivity: Avevena sativa

V-ATPase, subunit 36 kDa (Avena sativa) (clone 7A3) in the group Antibodies Plant/Algal / Membrane Transport System / Vacuolar membrane at Agrisera AB (Antibodies for research) (AS17 4163)

Product Information

Immunogen V-ATPase complex from Avena sativa purified by gel filtration Ward and Sze 1992

Host Mouse

Clonality Monoclonal, clone 7A3

Subclass/isotype IgG

Purity Cell culture supernatant.

Format Liquid

Quantity 1 ml

Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications

Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 1 : 50 - 1: 100 (WB)

Expected | apparent MW 36 kDa

Reactivity

Confirmed reactivity Avena sativa

Predicted reactivity

Avena sativa

Not reactive in other monocots

Application examples

Additional information

Background

Background V-ATPase subunit A is a catalytic subunit of V1 complex of vacuolar ATPase. This enzyme (EC=3.6.3.14) is involved in acidification process of various compartements of eucaryotic cell. This protein is coded by VHA-A gene. Alternative names: Vacuolar proton pump subunit alpha, vacuolar H(+)-ATPase subunit A, V-ATPase 69 kDa subunit

Product citations

Selected references Li and Sze (1999). A 100 kDa polypeptide associates with the V0 membrane sector but not with the active oat vacuolar H(+)-ATPase, suggesting a role in assembly. Plant J. 1999 Jan;17(1):19-30.
Li and Sze (1999). A 100 kDa polypeptide associates with the V0 membrane sector but not with the active oat vacuolar H(+)-ATPase, suggesting a role in assembly. Plant J. 1999 Jan;17(1):19-30.

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immunogen:	V-ATPase complex from Avena sativa purified by gel filtration Ward and Sze 1992
Sub class:	IgG
Host:	Mouse
Clonality:	Monoclonal, clone 7A3
Purity:	Cell culture supernatant.
Format:	Liquid
Quantity:	1 ml
storage:	Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Immunoprecipitation (IP), Western blot (WB)
recommended dilution:	1 : 50 - 1: 100 (WB)
calculated \| apparent molecular mass [kDa]:	36 kDa
Confirmed reactivity:	Avena sativa
predicted reactivity:	Avena sativa
not reactive in:	other monocots
background:	V-ATPase subunit A is a catalytic subunit of V1 complex of vacuolar ATPase. This enzyme (EC=3.6.3.14) is involved in acidification process of various compartements of eucaryotic cell. This protein is coded by VHA-A gene. Alternative names: Vacuolar proton pump subunit alpha, vacuolar H(+)-ATPase subunit A, V-ATPase 69 kDa subunit
All references:	Li and Sze (1999). A 100 kDa polypeptide associates with the V0 membrane sector but not with the active oat vacuolar H(+)-ATPase, suggesting a role in assembly. Plant J. 1999 Jan;17(1):19-30. Li and Sze (1999). A 100 kDa polypeptide associates with the V0 membrane sector but not with the active oat vacuolar H(+)-ATPase, suggesting a role in assembly. Plant J. 1999 Jan;17(1):19-30.