ASY1 | Asynaptic phenotype protein 1 (monoclonal)

Name: ASY1 | Asynaptic phenotype protein 1 (monoclonal)
Brand: IKP
SKU: AS08 338
Price: 349 EUR
Availability: InStock

AS08 338 | Clonality: Monoclonal | Host: Mouse | Reactivity: Triticum aestivum

ASY1 | Asynaptic phenotype protein 1 (monoclonal) in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / Cell cycle at Agrisera AB (Antibodies for research) (AS08 338)

ASY1 | Asynaptic phenotype protein 1 (monoclonal)

Product Information

Immunogen

KLH-conjugated synthetic pepitde chosen from Triticum aestivum ASY1 UniProt: A7TVU8

Host Mouse

Clonality Monoclonal

Purity Total IgG. Protein G purified purified from Cell culture supernatant.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water/tube

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution

1: 10 000 (ELISA), 1: 1000 (WB)

Expected | apparent MW 66,3 kDa

Reactivity

Confirmed reactivity Triticum aestivum

Predicted reactivity

Triticum aestivum

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

This antibody does not work in immunolocalization

Background

Pairing and synapsis of homologous chromosomes is required for normal chromosome segregation and the exchange of genetic material via recombination during meiosis. Synapsis is complete at pachytene following the formation of a tri-partite proteinaceous structure known as the synaptonemal complex (SC). In yeast, HOP1 is essential for formation of the SC, and localises along chromosome axes during prophase I. Homologues in Arabidopsis (AtASY1), Brassica (BoASY1) and rice (OsPAIR2) have been isolated through analysis of mutants that display decreased fertility due to severely reduced synapsis of homologous chromosomes. Analysis of these genes has indicated that they play a similar role to HOP1 in pairing and formation of the SC through localisation to axial/lateral elements of the SC.

Product citations

Selected references Lewandowska et al. (2021) The proteome of developing barley anthers during meiotic prophase I. J Exp Bot. 2021 Nov 10:erab494. doi: 10.1093/jxb/erab494. Epub ahead of print. PMID: 34758083.
Darrier et al. (2019). Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High-Resolution Microscopy. Methods Mol Biol. 2020;2061:207-215. doi: 10.1007/978-1-4939-9818-0_15.

immunogen:	KLH-conjugated synthetic pepitde chosen from Triticum aestivum ASY1 UniProt: A7TVU8
Reconstitution:	For reconstitution add 50 µl of sterile water/tube
Host:	Mouse
Clonality:	Monoclonal
Purity:	Total IgG. Protein G purified purified from Cell culture supernatant.
Format:	Lyophilized
Quantity:	50 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Western blot (WB)
recommended dilution:	1: 10 000 (ELISA), 1: 1000 (WB)
calculated \| apparent molecular mass [kDa]:	66,3 kDa
Confirmed reactivity:	Triticum aestivum
predicted reactivity:	Triticum aestivum
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information (application):	This antibody does not work in immunolocalization
background:	Pairing and synapsis of homologous chromosomes is required for normal chromosome segregation and the exchange of genetic material via recombination during meiosis. Synapsis is complete at pachytene following the formation of a tri-partite proteinaceous structure known as the synaptonemal complex (SC). In yeast, HOP1 is essential for formation of the SC, and localises along chromosome axes during prophase I. Homologues in Arabidopsis (AtASY1), Brassica (BoASY1) and rice (OsPAIR2) have been isolated through analysis of mutants that display decreased fertility due to severely reduced synapsis of homologous chromosomes. Analysis of these genes has indicated that they play a similar role to HOP1 in pairing and formation of the SC through localisation to axial/lateral elements of the SC.
All references:	Lewandowska et al. (2021) The proteome of developing barley anthers during meiotic prophase I. J Exp Bot. 2021 Nov 10:erab494. doi: 10.1093/jxb/erab494. Epub ahead of print. PMID: 34758083. Darrier et al. (2019). Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High-Resolution Microscopy. Methods Mol Biol. 2020;2061:207-215. doi: 10.1007/978-1-4939-9818-0_15.