ASyM | Mouse anti-human alpha synuclein N-terminal (clone number 4,2)
AS13 2719 | Clonality: monoclonal | Host: Mouse | Reactivity: Human
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Product Information
Immunogen
synthetic peptide derived from human alpha-synuclein N-terminal Met1-Val15
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG2a
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 ĩg
Reconstitution
For reconstitution add 50 ĩl of sterile water
Storage
For short time storage add sodium azide and store at +4°C. For long time storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC)
Recommended dilution
1-2 ug/ml (Dot), 2-4 ug/ml (ELISA capture), 10 ug/ml (IHC)
Expected | apparent MW
14 kDa
Reactivity
Confirmed reactivity
Human
Predicted reactivity
Mouse
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Additional information
This antibody will recognize human SNCA monomers and multimers in Western blot.
This antibody binds weakly to fibrills in IHC.
Cross reactivity of this antibody to synuclein beta was not determined.
This antibody can be used as a capture antibody in ELISA, combined with AS08 358 as a detection antibody.
Background
Background
Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy.
Product citations
Selected references
Tanudjojo et al. (2021) Phenotypic manifestation of ?-synuclein strains derived from Parkinson's disease and multiple system atrophy in human dopaminergic neurons. Nat Commun. 2021 Jun 21;12(1):3817. doi: 10.1038/s41467-021-23682-z. PMID: 34155194; PMCID: PMC8217249.
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