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ASyO2 | Mouse anti-human alpha-synuclein | oligomer-specific (clone number 51,24)

AS13 2717  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Human

ASyO2 | Mouse anti-human alpha-synuclein | oligomer-specific  (clone number 51,24) in the group Antibodies Human Research / Neuroscience / Neurodegenerative diseases / Parkinson's disease at Agrisera AB (Antibodies for research) (AS13 2717)



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Product Information

Immunogen synthetic peptide derived from human alpha-synuclein Glu131-Ala140
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage For short time storage please add sodium azide and srote at +4°C.For long time storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Dot blot (Dot), ELISA (ELISA), Immunolocalization (IL)
Recommended dilution 1-2 ug/ml (Dot), 2-4 ug/ml (ELISA capture), 10 ug/ml (IHC)
Expected | apparent MW

14 kDa

Reactivity

Confirmed reactivity Human
Predicted reactivity Mouse
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

dot blot
dot blot using anti-ASyO2 antibodies
Dot blot reaction of the binding capacity of ASyO2 to fibrils, monomers and oligomers. Equal amounts of each sample were spotted on a nitrocellulose membrane and then dried. The membrane was blocked with 5% non-fat milk before incubated for 1 h with anti-ASyO2 (25nM) and then with secondary antibody, anti-mouse HRP-conjugated (1:1500). The membrane was washed with PBS containing 0.25% Tween-20 before detection using ECL prime (GE Healthcare).



immunolocalization

immunolocalization using anti ASyO2 antibodies

Tissue sections from the human PD midbrain, substantia nigra, were de-waxed and rehydrated in ethanol and then incubated with ASyO2 at RT for 1h. The immunoreactivity was detected with the anti-mouse Peroxidase Reagent Kit (ImmPRESS, Vector Laboratories, Inc.) and then developed using the ImmPACT AEC Peroxidase Substrate kit (Vector Laboratories, Inc.).

Additional information

Related products

Related products

AS13 2718 | anti-ASyO5 | Mouse anti-human alpha-synuclein | oligomer-specific, monoclonal antibodies
AS13 2719 | anti-ASyM | Mouse anti-human alpha synuclein N-terminal, monoclonal antibodies
AS13 2717B | anti-ASyO2-biotinylated | Mouse anti-human alpha-synuclein | oligomer-specific, monoclonal antibodies
AS13 2718B | anti-ASyO5-biotinylated |  Mouse anti-human alpha-synuclein | oligomer-specific, monoclonal antibodies
AS13 2719B | anti-ASyM-biotinylated | Mouse anti-human alpha synuclein N-terminal, monoclonal antibodies
AS08 358 | anti-SNCA | Alpha-synuclein, rabbit antibodies

Background

Background

Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy. In analogy to many other amyloid associated disorders, alpha-synuclein may also form oligomeric assemblies. These small and soluble forms have been suggested to exert a stronger tissue damaging effect as compared to the monomeric and fibrillar counterpart. Using a recently developed technique a monoclonal oligomer-specific antibody has been designed.  

Product citations

Selected references Brännström et al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.

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