SNCA | Alpha-synuclein

Name: SNCA | Alpha-synuclein
SKU: AS08 358
Price: 333 EUR
Availability: InStock

AS08 358 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human

SNCA | Alpha-synuclein in the group Antibodies Human Cell Biology / Neuroscience / Neurodegenerative diseases / Parkinson's disease at Agrisera AB (Antibodies for research) (AS08 358)

Product Information

Immunogen recombinant, full length, human alpha-synuclein UniProt: P37840, epitope was mapped between amino acid 1-15

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunolocalization (IL), Western blot (WB)

Recommended dilution 1 : 3000 (ELISA), 1-2 µg/ml (IL), 1 : 1000 (WB)

Expected | apparent MW

14 kDa

Reactivity

Confirmed reactivity Human

Predicted reactivity Primates, Chimpanzee, Gorilla

Application examples

Western analysis

• Membrane: Nitrocellulose
• Blocking buffer: 5% dry milk in PBS, 0.15% tween 20
• Antibody dilution: 1:1000
• Secondary antibody: anti-rabbit (HRP)
• Detection: Enhanced Chemoluminescence (pico) 10s

1. Cell lysate lacking alpha-synuclein 10 000 ng (negative control)
2. Human plasma 10 000 ng (negative control)
3. Human alpha-synuclein 100 ng
4. Human alpha-synuclien 10 ng

Additional information

Immunolocalization: human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95°C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit.

This antibody will recognize human SNCA monomers and multimers in Western blot and can be used to detect fibrills in a sandwich ELISA.

In ELISA this antibody can be used for detection, combined with AS13 2719 as a capture antibody.

Background

Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy.

Product citations

Selected references Bargar et al. (2021) Discrimination of MSA-P and MSA-C by RT-QuIC analysis of olfactory mucosa: the first assessment of assay reproducibility between two specialized laboratories. Mol Neurodegener. 2021 Dec 11;16(1):82. doi: 10.1186/s13024-021-00491-y. PMID: 34895275; PMCID: PMC8665327.
Brännström et al. (2014). A generic method for design of oligomer-specific antibodies. PLoS One. 2014 Mar 11;9(3):e90857. doi: 10.1371/journal.pone.0090857. eCollection 2014.

immunogen:	recombinant, full length, human alpha-synuclein UniProt: P37840, epitope was mapped between amino acid 1-15
Reconstitution:	For reconstitution add 100 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	100 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
recommended dilution:	1 : 3000 (ELISA), 1-2 µg/ml (IL), 1 : 1000 (WB)
calculated \| apparent molecular mass [kDa]:	14 kDa
Confirmed reactivity:	Human
predicted reactivity:	Primates, Chimpanzee, Gorilla
Picture (footer):	Western analysis • Membrane: Nitrocellulose • Blocking buffer: 5% dry milk in PBS, 0.15% tween 20 • Antibody dilution: 1:1000 • Secondary antibody: anti-rabbit (HRP) • Detection: Enhanced Chemoluminescence (pico) 10s 1. Cell lysate lacking alpha-synuclein 10 000 ng (negative control) 2. Human plasma 10 000 ng (negative control) 3. Human alpha-synuclein 100 ng 4. Human alpha-synuclien 10 ng
additional information:	Immunolocalization: human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95°C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit. This antibody will recognize human SNCA monomers and multimers in Western blot and can be used to detect fibrills in a sandwich ELISA. In ELISA this antibody can be used for detection, combined with AS13 2719 as a capture antibody.
background:	Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy.
All references:	Bargar et al. (2021) Discrimination of MSA-P and MSA-C by RT-QuIC analysis of olfactory mucosa: the first assessment of assay reproducibility between two specialized laboratories. Mol Neurodegener. 2021 Dec 11;16(1):82. doi: 10.1186/s13024-021-00491-y. PMID: 34895275; PMCID: PMC8665327. Brännström et al. (2014). A generic method for design of oligomer-specific antibodies. PLoS One. 2014 Mar 11;9(3):e90857. doi: 10.1371/journal.pone.0090857. eCollection 2014.