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SNCA | Alpha-synuclein

AS08 358  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Human

SNCA | Alpha-synuclein in the group Antibodies for Human/Animal  / Human Proteins / Amyloid disorders / Parkinson's disease at Agrisera AB (Antibodies for research) (AS08 358)

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Product Information

Immunogen recombinant, full length, human alpha-synuclein UniProt: P37840, epitope was mapped between amino acid 1-15
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 3000 (ELISA), 1-2 µg/ml (IL), 1 : 1000 (WB)
Expected | apparent MW

14 kDa

Reactivity

Confirmed reactivity Human
Predicted reactivity Primates, Chimpanzee, Gorilla
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Western analysis


• Membrane: Nitrocellulose
• Blocking buffer: 5% dry milk in PBS, 0.15% tween 20
• Antibody dilution: 1:1000
• Secondary antibody: anti-rabbit (HRP)
• Detection: Enhanced Chemoluminescence (pico) 10s

 

 

1. Cell lysate lacking alpha-synuclein 10 000 ng (negative control)
2. Human plasma 10 000 ng (negative control)
3. Human alpha-synuclein 100 ng
4. Human alpha-synuclien 10 ng

Additional information

Additional information

Immunolocalization: human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95°C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit.

Related products

Related products

AS13 2718 | Anti-ASyO5 | Mouse anti-human alpha-synuclein | oligomer-specific, monoclonal antibodies
AS13 2719 | Anti-ASyM | Mouse anti-human alpha synuclein N-terminal, monoclonal antibodies

AS13 2717B | Anti-ASyO2-biotinylated | Mouse anti-human alpha-synuclein | oligomer-specific, monoclonal antibodies
AS13 2718B | Anti-ASyO5-biotinylated |  Mouse anti-human alpha-synuclein | oligomer-specific, monoclonal antibodies
AS13 2719B | Anti-ASyM-biotinylated | Mouse anti-human alpha synuclein N-terminal, monoclonal antibodies

Secondary antibodies

Background

Background

Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy.

Product citations

Selected references Brännström et al. (2014). A generic method for design of oligomer-specific antibodies. PLoS One. 2014 Mar 11;9(3):e90857. doi: 10.1371/journal.pone.0090857. eCollection 2014.

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