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ASyO5 | Mouse anti-human alpha-synuclein | oligomer-specific (clone number 2.4)

AS13 2718  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Human

ASyO5 | Mouse anti-human alpha-synuclein | oligomer-specific  (clone number 2.4) in the group Antibodies for Human/Animal  / Human Proteins / Amyloid disorders / Parkinson's disease at Agrisera AB (Antibodies for research) (AS13 2718)

DATA SHEET IN PDF

Qty: 
585
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

synthetic peptide derived from human alpha-synuclein Gly111–Tyr125 

Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Affinity purified in PBS pH 7.4, no preservatives
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage

For short time storage add sodium azide and store at +4°C.
For long time storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC)
Recommended dilution 1-2 ug/ml (Dot), 2-4 ug/ml (ELISA capture), 10 ug/ml (IHC)
Expected | apparent MW

14 kDa

Reactivity

Confirmed reactivity Human, mouse
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

dot blot
dot blot using ASyO5
Dot blot reaction of the binding capacity of ASyO5 to fibrils, monomers and oligomers. Equal amounts of each sample were spotted on a nitrocellulose membrane and then dried. The membrane was blocked with 5% non-fat milk before incubated for 1 h with anti-ASyO5 (25nM) and then with secondary antibody, anti-mouse HRP-conjugated (1:1500). The membrane was washed with PBS containing 0.25% Tween-20 before detection using ECL prime (GE Healthcare).


Immunolocalization

immunolocalization using anti-ASyO5 antibodies

Tissue sections from the human PD midbrain, substantia nigra, were de-waxed and rehydrated in ethanol and then incubated with ASyO5 at RT for 1h. The immunoreactivity was detected with the anti-mouse Peroxidase Reagent Kit (ImmPRESS, Vector Laboratories, Inc.) and then developed using the ImmPACT AEC Peroxidase Substrate kit (Vector Laboratories, Inc.).


Additional information

Additional information

This antibody is specific to oligomers in ELISA as a capture antibody. For specific details, please check: Brännström et al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.

Related products

Related products

AS13 2719 | anti-ASyM | Mouse anti-human alpha synuclein N-terminal, monoclonal antibodies
AS13 2717B | anti-ASyO2-biotinylated | Mouse anti-human alpha-synuclein | oligomer-specific, monoclonal antibodies
AS13 2718B | anti-ASyO5-biotinylated |  Mouse anti-human alpha-synuclein | oligomer-specific, monoclonal antibodies
AS13 2719B | anti-ASyM-biotinylated | Mouse anti-human alpha synuclein N-terminal, monoclonal antibodies
AS08 358 | anti-SNCA | Alpha-synuclein, rabbit antibodies

Secondary antibodies

Background

Background

Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy. In analogy to many other amyloid associated disorders, alpha-synuclein may also form oligomeric assemblies. These small and soluble forms have been suggested to exert a stronger tissue damaging effect as compared to the monomeric and fibrillar counterpart. Using a recently developed technique a monoclonal oligomer-specific antibody has been designed.  

Product citations

Selected references Kilpeläinen et al. (2019). Behavioural and dopaminergic changes in double mutated human A30P*A53T alpha-synuclein transgenic mouse model of Parkinson´s disease.Sci Rep. 2019 Nov 22;9(1):17382. doi: 10.1038/s41598-019-54034-z.
Wu et al. (2017). The critical role of Nramp1 in degrading α-synuclein oligomers in microglia under iron overload condition. Neurobiol Dis. 2017 Aug;104:61-72. doi: 10.1016/j.nbd.2017.05.001. (human, mouse, immunolocalization)
Svarcbahs et al. (2016). Inhibition of Prolyl Oligopeptidase Restores Spontaneous Motor Behavior in the α-Synuclein Virus Vector-Based Parkinson's Disease Mouse Model by Decreasing α-Synuclein Oligomeric Species in Mouse Brain. J Neurosci. 2016 Dec 7;36(49):12485-12497.
Brännström et al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.

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