FtsH6 | ATP-dependent zinc metalloprotease FtsH6 (chloroplastic)
AS05 094A | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Product Information
KLH-conjugated peptide chosen from AtFtsH6 protein sequence of Arabidopsis thaliana Q1PDW5
Reactivity
Arabidopsis thaliana
Application examples

Reactant: Arabidopsis thaliana (Thale cress)
Application: Western Blotting
Pudmed ID: 27561243
Journal: Nat Commun
Figure Number: 6B
Published Date: 2016-08-26
First Author: Sedaghatmehr, M., Mueller-Roeber, B., et al.
Impact Factor: 13.783
Open PublicationMetalloprotease FtsH6 is involved in thermopriming.(a) FtsH6 expression in Arabidopsis N13 and Col-0 seedlings during the memory phase compared to unprimed controls. FC, fold change. Error bars indicate meansąs.d. of three independent biological replicates each containing a pool of ?160 seedlings. (b) Immunoblot analysis of FtsH6 protein in accessions Col-0 and N13 at different time points of the memory phase. Immunodetection was performed using anti-FtsH6 antibody (top panels). RbcL, Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit (loading control; bottom panels). Note the absence of FtsH6 protein in Col-0 unprimed samples and in N13 unprimed and primed samples. (c) Immunoblot analysis of HSP21 protein in ftsh6 mutant (Salk_012429) and Col-0 seedlings after the first step of the priming treatment (90?min, 37?°C) and during the thermomemory phase. Immunodetection was performed using anti-HSP21 antibody (top panel). RbcL, ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit (loading control; bottom panel). (d) Signals of the immunoblot analyses were quantified using ImageJ and normalized to the amount of RbcL in the same samples. Meansąs.d. are given (n=3, independent biological replicates each representing a pool of ?100 seedlings grown on five plates; gel blots used for the quantification are shown in Supplementary Fig. 10). Asterisks indicate statistically significant difference (P<0.05; Student's t-test) from the Col-0 control. kDa, kilo Dalton.

Reactant: Arabidopsis thaliana (Thale cress)
Application: Western Blotting
Pudmed ID: 27561243
Journal: Nat Commun
Figure Number: 6C
Published Date: 2016-08-26
First Author: Sedaghatmehr, M., Mueller-Roeber, B., et al.
Impact Factor: 13.783
Open PublicationMetalloprotease FtsH6 is involved in thermopriming.(a) FtsH6 expression in Arabidopsis N13 and Col-0 seedlings during the memory phase compared to unprimed controls. FC, fold change. Error bars indicate meansąs.d. of three independent biological replicates each containing a pool of ?160 seedlings. (b) Immunoblot analysis of FtsH6 protein in accessions Col-0 and N13 at different time points of the memory phase. Immunodetection was performed using anti-FtsH6 antibody (top panels). RbcL, Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit (loading control; bottom panels). Note the absence of FtsH6 protein in Col-0 unprimed samples and in N13 unprimed and primed samples. (c) Immunoblot analysis of HSP21 protein in ftsh6 mutant (Salk_012429) and Col-0 seedlings after the first step of the priming treatment (90?min, 37?°C) and during the thermomemory phase. Immunodetection was performed using anti-HSP21 antibody (top panel). RbcL, ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit (loading control; bottom panel). (d) Signals of the immunoblot analyses were quantified using ImageJ and normalized to the amount of RbcL in the same samples. Meansąs.d. are given (n=3, independent biological replicates each representing a pool of ?100 seedlings grown on five plates; gel blots used for the quantification are shown in Supplementary Fig. 10). Asterisks indicate statistically significant difference (P<0.05; Student's t-test) from the Col-0 control. kDa, kilo Dalton.
Additional information
Background
Degradation of the most abundant membrane protein on earth, the light-harvesting complex of Photosystem II (LHC II), is highly regulated under various environmental conditions, e.g., light stress, to prevent photochemical damage to the reaction center. FtsH6 is proposed to be a general LHC II protease and FtsH6-dependent LHC II proteolysis can be a feature of all higher plants.
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