FtsH6 | ATP-dependent zinc metalloprotease FtsH6 (chloroplastic)
AS05 094A | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Product Information
Immunogen
KLH-conjugated peptide chosen from AtFtsH6 protein sequence of Arabidopsis thaliana Q1PDW5
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified serum
Format
Lyophilized in PBS pH 7.4
Quantity
2x100 µg
Reconstitution
For reconstitution add 91 µl of sterile water/tube.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1 500-1 : 1000 (WB)
Expected | apparent MW
74.36 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Background
Background
Degradation of the most abundant membrane protein on earth, the light-harvesting complex of Photosystem II (LHC II), is highly regulated under various environmental conditions, e.g., light stress, to prevent photochemical damage to the reaction center. FtsH6 is proposed to be a general LHC II protease and FtsH6-dependent LHC II proteolysis can be a feature of all higher plants.
Product citations
Selected references
Sedaghatmehr et al. (2016). The plastid metalloprotease FtsH6 and small heat shock protein HSP21 jointly regulate thermomemory in Arabidopsis. Nat Commun. 2016 Aug 26;7:12439. doi: 10.1038/ncomms12439.
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