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FtsH6 | ATP-dependent zinc metalloprotease FtsH6 (chloroplastic)

AS05 094A  |  Clonality: Polyclonal  | Host: Rabbit  |  Reactivity: Arabidopsis thaliana

FtsH6 | ATP-dependent zinc metalloprotease FtsH6 (chloroplastic) in the group Antibodies Plant/Algal  / Photosynthesis  / Proteases at Agrisera AB (Antibodies for research) (AS05 094A)
FtsH6 | ATP-dependent zinc metalloprotease FtsH6 (chloroplastic)



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Product Information

Immunogen

KLH-conjugated  peptide chosen from AtFtsH6 protein sequence of Arabidopsis thaliana  Q1PDW5

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 2x100 ĩg
Reconstitution For reconstitution add 91 ĩl of sterile water/tube
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1 500-1 : 1000 (WB)
Expected | apparent MW 74,36 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity

Arabidopsis thaliana

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Reactant: Arabidopsis thaliana (Thale cress)

Application: Western Blotting

Pudmed ID: 27561243

Journal: Nat Commun

Figure Number: 6B

Published Date: 2016-08-26

First Author: Sedaghatmehr, M., Mueller-Roeber, B., et al.

Impact Factor: 13.783

Open Publication

Metalloprotease FtsH6 is involved in thermopriming.(a) FtsH6 expression in Arabidopsis N13 and Col-0 seedlings during the memory phase compared to unprimed controls. FC, fold change. Error bars indicate meansąs.d. of three independent biological replicates each containing a pool of ?160 seedlings. (b) Immunoblot analysis of FtsH6 protein in accessions Col-0 and N13 at different time points of the memory phase. Immunodetection was performed using anti-FtsH6 antibody (top panels). RbcL, Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit (loading control; bottom panels). Note the absence of FtsH6 protein in Col-0 unprimed samples and in N13 unprimed and primed samples. (c) Immunoblot analysis of HSP21 protein in ftsh6 mutant (Salk_012429) and Col-0 seedlings after the first step of the priming treatment (90?min, 37?°C) and during the thermomemory phase. Immunodetection was performed using anti-HSP21 antibody (top panel). RbcL, ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit (loading control; bottom panel). (d) Signals of the immunoblot analyses were quantified using ImageJ and normalized to the amount of RbcL in the same samples. Meansąs.d. are given (n=3, independent biological replicates each representing a pool of ?100 seedlings grown on five plates; gel blots used for the quantification are shown in Supplementary Fig. 10). Asterisks indicate statistically significant difference (P<0.05; Student's t-test) from the Col-0 control. kDa, kilo Dalton.


Reactant: Arabidopsis thaliana (Thale cress)

Application: Western Blotting

Pudmed ID: 27561243

Journal: Nat Commun

Figure Number: 6C

Published Date: 2016-08-26

First Author: Sedaghatmehr, M., Mueller-Roeber, B., et al.

Impact Factor: 13.783

Open Publication

Metalloprotease FtsH6 is involved in thermopriming.(a) FtsH6 expression in Arabidopsis N13 and Col-0 seedlings during the memory phase compared to unprimed controls. FC, fold change. Error bars indicate meansąs.d. of three independent biological replicates each containing a pool of ?160 seedlings. (b) Immunoblot analysis of FtsH6 protein in accessions Col-0 and N13 at different time points of the memory phase. Immunodetection was performed using anti-FtsH6 antibody (top panels). RbcL, Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit (loading control; bottom panels). Note the absence of FtsH6 protein in Col-0 unprimed samples and in N13 unprimed and primed samples. (c) Immunoblot analysis of HSP21 protein in ftsh6 mutant (Salk_012429) and Col-0 seedlings after the first step of the priming treatment (90?min, 37?°C) and during the thermomemory phase. Immunodetection was performed using anti-HSP21 antibody (top panel). RbcL, ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit (loading control; bottom panel). (d) Signals of the immunoblot analyses were quantified using ImageJ and normalized to the amount of RbcL in the same samples. Meansąs.d. are given (n=3, independent biological replicates each representing a pool of ?100 seedlings grown on five plates; gel blots used for the quantification are shown in Supplementary Fig. 10). Asterisks indicate statistically significant difference (P<0.05; Student's t-test) from the Col-0 control. kDa, kilo Dalton.

Additional information

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Antibodies to other proteins involved in photosynthesis

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background

Degradation of the most abundant membrane protein on earth, the light-harvesting complex of Photosystem II (LHC II), is highly regulated under various environmental conditions, e.g., light stress, to prevent photochemical damage to the reaction center. FtsH6 is proposed to be a general LHC II protease and FtsH6-dependent LHC II proteolysis can be  a feature of all higher plants.

Product citations

Selected references Sedaghatmehr et al. (2022) Heat shock factor HSFA2 fine-tunes resetting of thermomemory via plastidic metalloprotease FtsH6. J Exp Bot. 2022 Jun 15:erac257. doi: 10.1093/jxb/erac257. Epub ahead of print. PMID: 35705109.
Sedaghatmehr et al. (2016). The plastid metalloprotease FtsH6 and small heat shock protein HSP21 jointly regulate thermomemory in Arabidopsis. Nat Commun. 2016 Aug 26;7:12439. doi: 10.1038/ncomms12439.

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