AtpA | Alpha subunit of ATP synthase, chloroplastic (plant)
AS08 304 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. modesticaldum, Z. mays
|Data sheet||Product citations||Protocols||Add review|
Recombinant maize chloroplast AtpA P05022
Species of your interest not listed? Contact us
Reactant: Vitis vinifera (Common grape vine)
Application: Western Blotting
Pudmed ID: 31156689
Journal: Front Plant Sci
Figure Number: 2F
Published Date: 2019-06-04
First Author: Kuang, L., Chen, S., et al.
Impact Factor: 5.435Open Publication
Grape berry flesh cell protoplast and vacuole preparations and quality check. (A) Isolated protoplasts. (B) Vacuole enrichment by Ficoll gradient centrifugation. (C) Crude vacuoles stained with Neutral Red. (D) Vacuoles in vacuole-enriched preparation stained with Neutral Red, solid arrow indicating vacuole, dashed arrow indicating tonoplast vesicle, inset indicating vacuoles with microdomains. (E) Relative quantity of P-ATPase between protoplast and vacuole isolations by western blotting analysis. (F) Relative quantity of V-ATPase between protoplast and vacuole isolations by western blotting analysis. Values were the average of three replicates ą SD.
Sequence of the protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.
Zhang et al. (2017). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989.
Jeon et al. (2017). Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Plant Mol Biol. 2017 Nov;95(4-5):463-479. doi: 10.1007/s11103-017-0664-y.
Murcha et al. (2016). Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016.
Camejo et al. (2015). Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits. Proteomics. 2015 Aug;15(15):2634-42. doi: 10.1002/pmic.201400370.
Yang et al. (2015). Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 May 12. pii: S1046-5928(15)00111-4. doi: 10.1016/j.pep.2015.05.006.
Related products: AtpA | Alpha subunit of ATP synthase, chloroplastic (plant)
This product can b...