AtpA | Alpha subunit of ATP synthase, chloroplastic (plant)

Name: AtpA | Alpha subunit of ATP synthase, chloroplastic (plant)
SKU: AS08 304
Price: 394 EUR
Availability: InStock

AS08 304 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. modesticaldum, O. sativa, Z. mays

AtpA | Alpha subunit of ATP synthase, chloroplastic (plant) in the group Antibodies Plant/Algal / Photosynthesis / ATP synthase at Agrisera AB (Antibodies for research) (AS08 304)

AtpA | Alpha subunit of ATP synthase, chloroplastic (plant)

Product Information

Immunogen

Recombinant maize chloroplast AtpA P05022

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunogold (IG), Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 1: 200 (IG), 1 : 5 000 (WB)

Expected | apparent MW

55 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Begonia sp., Capsicum annuum, Heliobacterium modesticaldum, Oryza sativa, Zea mays

Predicted reactivity Dicots and (including Lilium superbum) chloroplast AtpA; may cross-react with mitochondrial AtpA; Algae, Nannochloropsis gaditana, Cyanobacteria
Species of your interest not listed? Contact us

Not reactive in Nicotiana benthamiana

Application examples

Application examples Application example

AS08 304 western blot picture.jpg

Western blot

Lane 1: Arabidopsis thaliana total leaf protein extract;

Lane 2: Zea mays total leaf protein extract

5 µg of total protein were loaded per each lane.

Experimental conditions:

Proteins were separated in a 12% or 5-15% gradient gel following by a transfer to nitrocellulose membrane. Membrane was blocked with TBST + 4% non-fat dried milk, 20 min following by three washes in TBST. Incubation time with primary and secondary antibodies was 1 hr primary, 30 min for secondary antibodies followed by reaction development using chemiluminescence.

Reactant: Vitis vinifera (Common grape vine)

Application: Western Blotting

Pudmed ID: 31156689

Journal: Front Plant Sci

Figure Number: 2F

Published Date: 2019-06-04

First Author: Kuang, L., Chen, S., et al.

Impact Factor: 5.435

Open Publication

Grape berry flesh cell protoplast and vacuole preparations and quality check. (A) Isolated protoplasts. (B) Vacuole enrichment by Ficoll gradient centrifugation. (C) Crude vacuoles stained with Neutral Red. (D) Vacuoles in vacuole-enriched preparation stained with Neutral Red, solid arrow indicating vacuole, dashed arrow indicating tonoplast vesicle, inset indicating vacuoles with microdomains. (E) Relative quantity of P-ATPase between protoplast and vacuole isolations by western blotting analysis. (F) Relative quantity of V-ATPase between protoplast and vacuole isolations by western blotting analysis. Values were the average of three replicates ± SD.

Additional information

Sequence of the protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757

Background

ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.

Product citations

Selected references Pao et al. (2018). Lamelloplasts and minichloroplasts in Begoniaceae: iridescence and photosynthetic functioning. J Plant Res. 2018 Mar 2. doi: 10.1007/s10265-018-1020-2. (ImmunoGold)
Zhang et al. (2017). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989.
Jeon et al. (2017). Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Plant Mol Biol. 2017 Nov;95(4-5):463-479. doi: 10.1007/s11103-017-0664-y.
Murcha et al. (2016). Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016.
Camejo et al. (2015). Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits. Proteomics. 2015 Aug;15(15):2634-42. doi: 10.1002/pmic.201400370.
Yang et al. (2015). Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 May 12. pii: S1046-5928(15)00111-4. doi: 10.1016/j.pep.2015.05.006.

immunogen:	Recombinant maize chloroplast AtpA P05022
Reconstitution:	For reconstitution add 50 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Immunogold (IG), Immunoprecipitation (IP), Western blot (WB)
recommended dilution:	1: 200 (IG), 1 : 5 000 (WB)
calculated \| apparent molecular mass [kDa]:	55 kDa
Confirmed reactivity:	Arabidopsis thaliana, Begonia sp., Capsicum annuum, Heliobacterium modesticaldum, Oryza sativa, Zea mays
predicted reactivity:	Dicots and (including Lilium superbum) chloroplast AtpA; may cross-react with mitochondrial AtpA; Algae, Nannochloropsis gaditana, Cyanobacteria Species of your interest not listed? Contact us
not reactive in:	Nicotiana benthamiana
Picture (footer):	Application example Western blot Lane 1: Arabidopsis thaliana total leaf protein extract; Lane 2: Zea mays total leaf protein extract 5 µg of total protein were loaded per each lane. Experimental conditions: Proteins were separated in a 12% or 5-15% gradient gel following by a transfer to nitrocellulose membrane. Membrane was blocked with TBST + 4% non-fat dried milk, 20 min following by three washes in TBST. Incubation time with primary and secondary antibodies was 1 hr primary, 30 min for secondary antibodies followed by reaction development using chemiluminescence.
More images:	Reactant: Vitis vinifera (Common grape vine) Application: Western Blotting Pudmed ID: 31156689 Journal: Front Plant Sci Figure Number: 2F Published Date: 2019-06-04 First Author: Kuang, L., Chen, S., et al. Impact Factor: 5.435 Open Publication Grape berry flesh cell protoplast and vacuole preparations and quality check. (A) Isolated protoplasts. (B) Vacuole enrichment by Ficoll gradient centrifugation. (C) Crude vacuoles stained with Neutral Red. (D) Vacuoles in vacuole-enriched preparation stained with Neutral Red, solid arrow indicating vacuole, dashed arrow indicating tonoplast vesicle, inset indicating vacuoles with microdomains. (E) Relative quantity of P-ATPase between protoplast and vacuole isolations by western blotting analysis. (F) Relative quantity of V-ATPase between protoplast and vacuole isolations by western blotting analysis. Values were the average of three replicates ± SD.
additional information:	Sequence of the protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757
background:	ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.
All references:	Pao et al. (2018). Lamelloplasts and minichloroplasts in Begoniaceae: iridescence and photosynthetic functioning. J Plant Res. 2018 Mar 2. doi: 10.1007/s10265-018-1020-2. (ImmunoGold) Zhang et al. (2017). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989. Jeon et al. (2017). Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Plant Mol Biol. 2017 Nov;95(4-5):463-479. doi: 10.1007/s11103-017-0664-y. Murcha et al. (2016). Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016. Camejo et al. (2015). Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits. Proteomics. 2015 Aug;15(15):2634-42. doi: 10.1002/pmic.201400370. Yang et al. (2015). Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 May 12. pii: S1046-5928(15)00111-4. doi: 10.1016/j.pep.2015.05.006.