ATPase AAA2 domain
AS11 1754 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C.reinhardtii, L. angustifolius, P. sativum, S. tuberosum, Synechococcus sp. PCC 7942, Z. mays L.
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Courtesy Dr. Elena Pojidaeva, Laboratory of Plant Gene Expression, Timiryazev Institute of Plant Physiology RAS, 127276 Moscow Russia
0.3 µg of total protein from Synechococcus sp. PCC7942 (1) and 20 µg of leaf soluble proteins from 3-week old Arabidopsis thaliana leaves (2) were separated on 3-8 % Tris-acetate NuPage gel (invitrogen) and blotted 1,5 h to supported introcellulose. Blots were blocked for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 in 1xTBS-T for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:75 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 120 seconds.
Courtesy of Dr. A. Clarke, Göteborg University, Sweden