TIP2 | Tonoplast intrinsic protein 2;1, 2;2, 2;3
AS12 2619 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana TIP2;1 (100 %) UniProt:Q41951, TAIR:AT3G16240, TIP2;2 (90%) UniProt: Q41975, TAIR:AT4G17340, TIP2;3 (81%) UniProt: Q9FGL2, TAIR: AT5G47450
25.6 | 24 kDa (Arabidopsis thaliana)
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10 µg of total proteinfrom Arabidopsis thaliana roots (Ecotype-Columbia) were separated on 12%SDS-PAGE and blotted 1h to PVDF membrane. Blots were blocked with 5%low-fat milk powderfor 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 over night with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T (0.05% Triton X.100)at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidaseconjugated from donkey diluted to 1:15 000 in for 1h at RT with agitation. The blot was washed as above and developed with chemiluminescent reagent, according to the manufacturer's instructions. Exposure time was 5 seconds.
Courtesy of Dr. Azeez Suliman Beebo, University of Gothenburg, Sweden
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
TIP2 (Aquaporin TIP) is a protein required for the transport of water from the vacuolar compartment to the cytoplasm. Alternative names: Delta-tonoplast intrinsic protein, Delta-TIP.