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TIP2 | Tonoplast intrinsic protein 2;1, 2;2, 2;3

AS12 2619 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

TIP2 | Tonoplast intrinsic protein 2;1, 2;2, 2;3 in the group Antibodies for Plant/Algal  / Membrane Transport System / Vacuolar membrane at Agrisera AB (Antibodies for research) (AS12 2619)

DATA SHEET IN PDF

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Product Information

Immunogen

KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana TIP2;1 (100 %) UniProt:Q41951, TAIR:AT3G16240, TIP2;2 (90%) UniProt: Q41975, TAIR:AT4G17340, TIP2;3 (81%) UniProt: Q9FGL2, TAIR: AT5G47450

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 200 µg
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), Western blot (WB)
Recommended dilution 1:100 (IL), 1 : 1000 -1 : 2000 (WB)
Expected | apparent MW

25.6 | 24 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Quercus suber
Predicted reactivity Antirrhinum majus, Brassica oleracea, Chorispora exscapa, Gossypium mexicanum, Helianthus annuus, Medicago truncatula, Malus prunifolia, Nicotiana tabacum, Populus balsamifera subsp. trichocarpa, Raphanus sativus, Ricinus communis, Solanum nigrum , Solanum tuberosum, Vitis vinifera, Quercus petraea
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example


10 µg of total proteinfrom Arabidopsis thaliana roots (Ecotype-Columbia) were separated on 12%SDS-PAGE and blotted 1h to PVDF membrane. Blots were blocked with 5%low-fat milk powderfor 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 over night with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T (0.05% Triton X.100)at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidaseconjugated from donkey diluted to 1:15 000 in  for 1h at RT with agitation. The blot was washed as above and developed with chemiluminescent reagent, according to the manufacturer's instructions. Exposure time was 5 seconds.

Courtesy of Dr. Azeez Suliman Beebo, University of Gothenburg, Sweden

Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Related products

Related products

collection of antibodies to tonoplast proteins

AS09 493 | Anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma), rabbit antibodies

AS09 494 | Anti-TIP2;1 (delta) | Delta-VM23, rabbit antibodies

AS09 510 | Anti-TIP2;1 | tonoplast intrinistic protein 2-1, rabbit antibodies

AS09 511 | Anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal), rabbit antibodies

Plant protein extraction buffer

Background

Background

TIP2  (Aquaporin TIP) is a protein required for the transport of water from the vacuolar compartment to the cytoplasm. Alternative names: Delta-tonoplast intrinsic protein, Delta-TIP.

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