BAM | Beta-amylase, Biotin conjugated
AS09 380 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Kappaphycus alvarezii, Solanum tuberosum
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Product Information
Immunogen
Beta amylase isolated and purified from sweet potato Q94EU9
Host
Rabbit
Clonality
Polyclonal
Purity
Purified IgG
Format
Lyophilized in PBS labelled with biotin
Quantity
1 ml (10 mg/ml)
Reconstitution
For reconstitution add 1 ml of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 1000-1 : 4000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW
60 kDa
Reactivity
Confirmed reactivity
Kappaphycus alvarezii, Solanum tuberosum
Predicted reactivity
Arabidopsis thaliana, Glycine max, Physcomitrella patens, Populus trichocarpa, Ricinus communis, Vitis vinifera
Application examples
Additional information
Additional information
Biotin/IgG protein molar ratio (B/P) is approximately 6.6. No foreign proteins are added. Marker used for lebling is N-hydroxysuccinimidoBiotin.
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Background
Background
Beta amylase (EC 3.2.1.2.) catalyzes the hydrolysis of the second alfa-1,4 glycosidic bond. Alternative names 1,4-alfa-D-glucan maltohydrolase, glycogenase,saccharogen. amylase).
Product citations
Selected references
Usuldin et al. (2017). Molecular investigation of carrageenan production in Kappaphycus alvarezii in different culture conditions: a proteomic approach. ournal of Applied Phycology, August 2017, Volume 29, Issue 4, pp 1989–2001. (Kappaphycus alvarezii)
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