BAM1 | Beta amylase 1 (chloroplastic)
AS15 2895 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Product Information
Immunogen
The mature length protein of Arabidopsis thaliana BAM1 overexpressed in E.coli, UniProt:Q9LIR6, TAIR: AT3G23920., lacking the transit peptide that is cleaved upon entry to the chloroplast. Recombinant protein had an N-terminal S-tag.
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 µl
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 7500 (WB)
Expected | apparent MW
63,7 | 60,9 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Beta vulgaris, Brassica napus, Brassica oleracea, Brassica rapa. , Camellia sinensis, Cajanus cajan, Capsella rubella, Citrus clementina, Citrus sinensis, Coffea canephora, Cucumis sativus, Cynara cardunculus var. scolymus, Daucus carota subsp. sativus, Eucalyptus grandis, Eutrema salsugineum, Glycine max, Gossypium arboreum,Hordeum vulgare, Jatropha curcas, Nicotiana tabacum, Phaseolus vulgaris, Poncirus trifoliata, Populus trichocarpa, Prunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Theobroma cacao, Vitis vinifera
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
application example

Arabidopsis thaliana leaf extract (5 ug total protein), BAM1 KO plant (1) and BAM3 KO which still has BAM1 protein (2). Extraction buffer (50 mM MOPS pH 7.0, 5 mM EDTA). Added Equal volume of SSB (final concentration: 50 mM Tris-HCl pH 6.8, 2.5% SDS, 15% glycerol, .05% bromophenol blue, 5% beta-mercaptoethanol) and boiled 5 min. Separated on 10% SDS-PAGE and blotted to nitrocellulose, 100V 1hr using tank transfer. 1 hr block 5% NFDM, PBST, RT 1 hr with primary antibody at 1: 7 500 in 5% NFDM, PBST, RT Washed with PBST 2x briefly, then 2x for 10 min 1 hr in secondary antibody at 1: 20 000, PBST, RT Washed with PBST 2x briefly, then 2x for 10 min chemiluminescent detection reagent. Expected BAM3 MW ~64 kDa (usually a doublet).
Courtesy of Dr. Amanda Storm, James Madison University, USA

Arabidopsis thaliana leaf extract (5 ug total protein), BAM1 KO plant (1) and BAM3 KO which still has BAM1 protein (2). Extraction buffer (50 mM MOPS pH 7.0, 5 mM EDTA). Added Equal volume of SSB (final concentration: 50 mM Tris-HCl pH 6.8, 2.5% SDS, 15% glycerol, .05% bromophenol blue, 5% beta-mercaptoethanol) and boiled 5 min. Separated on 10% SDS-PAGE and blotted to nitrocellulose, 100V 1hr using tank transfer. 1 hr block 5% NFDM, PBST, RT 1 hr with primary antibody at 1: 7 500 in 5% NFDM, PBST, RT Washed with PBST 2x briefly, then 2x for 10 min 1 hr in secondary antibody at 1: 20 000, PBST, RT Washed with PBST 2x briefly, then 2x for 10 min chemiluminescent detection reagent. Expected BAM3 MW ~64 kDa (usually a doublet).
Courtesy of Dr. Amanda Storm, James Madison University, USA
Additional information
Antibody is recognizing recombinant BAM1 protein of Arabidopsis thaliana.
Background
Background
Beta amylase (EC 3.2.1.2) is an enzyme involved in the hydrolysis fo starch into sugars. Can play a minor role in the starch degradation and maltose metabolism in chloroplasts during the night.
Product citations
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