Anti-BAM1 | Beta amylase 1 (chloroplastic)

Product no: AS15 2895

AS15 2895  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: The mature length protein of Arabidopsis thaliana BAM1 overexpressed in E.coli, UniProt:Q9LIR6, TAIR: AT3G23920, lacking the transit peptide that is cleaved upon entry to the chloroplast. Recombinant protein had an N-terminal S-tag.
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 7500 (WB)
    Expected | apparent MW: 63.7 | 60.9 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Beta vulgaris, Brassica napus, Brassica oleracea, Brassica rapa., Camellia sinensis, Cajanus cajan, Capsella rubella, Citrus clementina, Citrus sinensis, Coffea canephora, Cucumis sativus, Cynara cardunculus var. scolymus, Daucus carota subsp. sativus, Eucalyptus grandis, Eutrema salsugineum, Glycine max, Gossypium arboreum, Hordeum vulgare, Jatropha curcas, Nicotiana tabacum, Phaseolus vulgaris, Poncirus trifoliata, Populus trichocarpa, Prunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Theobroma cacao, Vitis vinifera

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    western blot using anti-beta amylase 1 antibodies

    Arabidopsis thaliana leaf extract (5 ug total protein), BAM1 KO plant (1) and BAM3 KO which still has BAM1 protein (2). Extraction buffer (50 mM MOPS pH 7.0, 5 mM EDTA). Added Equal volume of SSB (final concentration: 50 mM Tris-HCl pH 6.8, 2.5% SDS, 15% glycerol, .05% bromophenol blue, 5% beta-mercaptoethanol) and boiled 5 min. Separated on 10% SDS-PAGE and blotted to nitrocellulose, 100V 1hr using tank transfer. 1 hr block 5% NFDM, PBST, RT 1 hr with primary antibody at 1: 7 500 in 5% NFDM, PBST, RT Washed with PBST 2x briefly, then 2x for 10 min 1 hr in secondary antibody at 1: 20 000, PBST, RT Washed with PBST 2x briefly, then 2x for 10 min chemiluminescent detection reagent. Expected BAM3 MW ~64 kDa (usually a doublet).

    Courtesy of Dr. Amanda Storm, James Madison University, USA
  • Additional Information
  • Additional information (application):

    Antibody is recognizing recombinant BAM1 protein of Arabidopsis thaliana

  • Background
  • Background: Beta amylase (EC is an enzyme involved in the hydrolysis fo starch into sugars. Can play a minor role in the starch degradation and maltose metabolism in chloroplasts during the night.
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