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BAM1 | Beta amylase 1 (chloroplastic)

AS15 2895  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

BAM1 | Beta amylase 1 (chloroplastic) in the group Antibodies for Plant/Algal  / Photosynthesis  / Sucrose metabolism at Agrisera AB (Antibodies for research) (AS15 2895)

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Product Information

Immunogen The mature length protein of Arabidopsis thaliana BAM1 overexpressed in E.coli, UniProt:Q9LIR6, TAIR: AT3G23920., lacking the transit peptide that is cleaved upon entry to the chloroplast. Recombinant protein had an N-terminal S-tag.
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity

50 µl

Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 7500 (WB)
Expected | apparent MW

63.7 | 60.9  kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Beta vulgaris, Brassica napus, Brassica oleracea, Brassica rapa. , Camellia sinensis, Cajanus cajan, Capsella rubella,  Citrus clementina, Citrus sinensis, Coffea canephora, Cucumis sativus, Cynara cardunculus var. scolymus, Daucus carota subsp. sativus, Eucalyptus grandis, Eutrema salsugineum, Glycine max, Gossypium arboreum, Jatropha curcas, Nicotiana tabacum, Phaseolus vulgaris, Poncirus trifoliata, Populus trichocarpa, Prunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Theobroma cacao, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

western blot using anti-beta amylase 1 antibodies

Arabidopsis thaliana leaf extract (5 ug total protein), BAM1 KO plant (1) and BAM3 KO which still has BAM1 protein (2). Extraction buffer (50 mM MOPS pH 7.0, 5 mM EDTA). Added Equal volume of SSB (final concentration: 50 mM Tris-HCl pH 6.8, 2.5% SDS, 15% glycerol, .05% bromophenol blue, 5% beta-mercaptoethanol) and boiled 5 min. Separated on 10% SDS-PAGE and blotted to nitrocellulose, 100V 1hr using tank transfer. 1 hr block 5% NFDM, PBST, RT 1 hr with primary antibody at 1: 7 500 in 5% NFDM, PBST, RT Washed with PBST 2x briefly, then 2x for 10 min 1 hr in secondary antibody at 1: 20 000, PBST, RT Washed with PBST 2x briefly, then 2x for 10 min chemiluminescent detection reagent. Expected BAM3 MW ~64 kDa (usually a doublet).

Courtesy of Dr. Amanda Storm, James Madison University, USA

Additional information

Antibody is recognizing recombinant BAM1 protein of Arabidopsis thaliana

Related products

Related products AS15 2896 | Anti-BAM3 | Beta amylase 3, rabbit antibodies
antibodies to proteins involved in carbohydrate metabolism

Background

Background Beta amylase (EC 3.2.1.2) is an enzyme involved in the hydrolysis fo starch into sugars. Can play a minor role in the starch degradation and maltose metabolism in chloroplasts during the night.

Product citations

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