BAM1 | Beta amylase 1 (chloroplastic)

AS15 2895  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

BAM1 | Beta amylase 1 (chloroplastic) in the group Plant/Algal Antibodies / Photosynthesis  / Sucrose metabolism at Agrisera AB (Antibodies for research) (AS15 2895)


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product information
Background Beta amylase (EC is an enzyme involved in the hydrolysis fo starch into sugars. Can play a minor role in the starch degradation and maltose metabolism in chloroplasts during the night.
Immunogen The mature length protein of Arabidopsis thaliana BAM1 overexpressed in E.coli, UniProt:Q9LIR6, TAIR: AT3G23920., lacking the transit peptide that is cleaved upon entry to the chloroplast. Recombinant protein had an N-terminal S-tag.  
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized

50 µl

Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products AS15 2896 | anti-BAM3 | Beta amylase 3, rabbit antibodies
antibodies to proteins involved in carbohydrate metabolism
Additional information
application information
Recommended dilution 1 :7500 (WB)
Expected | apparent MW

63.7 | 60.9  kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Beta vulgaris, Brassica napus, Brassica oleracea, Brassica rapa. , Camellia sinensis, Cajanus cajan, Capsella rubella,  Citrus clementina, Citrus sinensis, Coffea canephora, Cucumis sativus, Cynara cardunculus var. scolymus, Daucus carota subsp. sativus, Eucalyptus grandis, Eutrema salsugineum, Glycine max, Gossypium arboreum, Jatropha curcas, Nicotiana tabacum, Phaseolus vulgaris, Poncirus trifoliata, Populus trichocarpa, Prunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Theobroma cacao, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Antibody is recognizing recombinant BAM1 protein of Arabidopsis thaliana

Selected references To be added when available, antibody released in September 2016.

application example

western blot using anti-beta amylase 1 antibodies

Arabidopsis thaliana leaf extract (5 ug total protein), BAM1 KO plant (1) and BAM3 KO which still has BAM1 protein (2). Extraction buffer (50 mM MOPS pH 7.0, 5 mM EDTA). Added Equal volume of SSB (final concentration: 50 mM Tris-HCl pH 6.8, 2.5% SDS, 15% glycerol, .05% bromophenol blue, 5% beta-mercaptoethanol) and boiled 5 min. Separated on 10% SDS-PAGE and blotted to nitrocellulose, 100V 1hr using tank transfer. 1 hr block 5% NFDM, PBST, RT 1 hr with primary antibody at 1: 7 500 in 5% NFDM, PBST, RT Washed with PBST 2x briefly, then 2x for 10 min 1 hr in secondary antibody at 1: 20 000, PBST, RT Washed with PBST 2x briefly, then 2x for 10 min Substrate WesternSurePremium (LiCor). Expected BAM3 MW ~64 kDa (usually a doublet).

Courtesy of Dr. Amanda Storm, James Madison University, USA

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