Beta-tubulin, (loading control antibody), mouse monoclonal
AS20 4382 | Clonality: Monoclonal | Host: Mouse | Reactivity: Human

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Product Information
Immunogen
Synthetic peptide derived from the N-terminal of the human beta-tubulin
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG2a
Purity
IgG2a purified on Protein A n 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
Format
Lyophilized
Quantity
100 ĩg
Storage
Store at -20°C for 3 years or more, Reconstitute with distilled water to desired concentration before use, Aliquot to avoid repeated freeze-thaw cycles, Store aliquots at 4°C for several days to weeks
Tested applications
Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1: 2000 - 1: 5000 (WB), for other techniques end user needs to determine most optimal diltion
Expected | apparent MW
50 kDa
Reactivity
Confirmed reactivity
Chicken, human, monkey, mouse, rat, rabbit
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Application example


Varied amounts of mouse brain tissue lysate: 10 µg/lane (1), 5 µg/lane (2), 2.5/lane µg (3), 1.25 µg/lane (4) were extracted and separated on SDS-PAGE and blotted to a membrane. Primary antibodies were used at a dilution of 1: 4000 (0.25 µg/ml). Reaction was developed by chemiluminescence.
Additional information
Additional information
10mM PBS, pH 7,2, with 1% BSA and 0,05% sodium azide
This antibody recognizes both the native and denatured forms of Beta-tubulin
Background
Background
Beta-tubulin is a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism. Tubulin is expressed at high levels in almost all tissues and cell lines. This makes it ideal to use as a loading control in Western blot.
Product citations
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