IKP

Anti-Beta-tubulin, (loading control antibody), mouse monoclonal

Product no: AS20 4382

AS20 4382 | Clonality: Monoclonal | Host: Mouse | Reactivity: Human

279
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen: Synthetic peptide derived from the N-terminal of the human beta-tubulin
    Sub class: IgG2a
    Host: Mouse
    Clonality: Monoclonal
    Purity: IgG2a purified on Protein A n 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
    Format: Lyophilized
    Quantity: 100 µg
    Storage: Store at -20°C for 3 years or more, Reconstitute with distilled water to desired concentration before use, Aliquot to avoid repeated freeze-thaw cycles, Store aliquots at 4°C for several days to weeks
    Tested applications: Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1: 2000 - 1: 5000 (WB), for other techniques end user needs to determine most optimal diltion
    Expected | apparent MW: 50 kDa
  • Reactivity
  • Confirmed reactivity: Chicken, human, monkey, mouse, rat, rabbit
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example


    Western blot using anti-beta tubulin antibodies

    Varied amounts of mouse brain tissue lysate: 10 µg/lane (1), 5 µg/lane (2), 2.5/lane µg (3), 1.25 µg/lane (4) were extracted and separated on SDS-PAGE and blotted to a membrane. Primary antibodies were used at a dilution of 1: 4000 (0.25 µg/ml). Reaction was developed by chemiluminescence. 

  • Additional Information
  • Additional information: 10mM PBS, pH 7,2, with 1% BSA and 0,05% sodium azide
    Additional information (application): This antibody recognizes both the native and denatured forms of Beta-tubulin
  • Background
  • Background: Beta-tubulin is a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism.  Tubulin is expressed at high levels in almost all tissues and cell lines. This makes it ideal to use as a loading control in Western blot.
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

AS09 627 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Mouse IgG (H&L)

218 €
Add to cart
Info
img missing

AS16 4022 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human

425 €
Add to cart
Info
img missing

AS20 4381 | Clonality: Monoclonal | Host: Mouse | Reactivity: Chicken, human, mouse, rabbit, rat

279 €
Add to cart
Info
Close