Beta-tubulin, (loading control antibody), mouse monoclonal

Name: Beta-tubulin, (loading control antibody), mouse monoclonal
Brand: IKP
SKU: AS20 4382
Price: 264 EUR
Availability: InStock

AS20 4382 | Clonality: Monoclonal | Host: Mouse | Reactivity: Human

Beta-tubulin, (loading control antibody), mouse monoclonal in the group Antibodies Human Cell Biology / Human Developmental Biology at Agrisera AB (Antibodies for research) (AS20 4382)

Product Information

Immunogen Synthetic peptide derived from the N-terminal of the human beta-tubulin

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG2a

Purity IgG2a purified on Protein A n 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.

Format Lyophilized

Quantity 100 µg

Storage Store at -20°C for 3 years or more, Reconstitute with distilled water to desired concentration before use, Aliquot to avoid repeated freeze-thaw cycles, Store aliquots at 4°C for several days to weeks

Tested applications Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1: 2000 - 1: 5000 (WB), for other techniques end user needs to determine most optimal diltion

Expected | apparent MW 50 kDa

Reactivity

Confirmed reactivity Chicken, human, monkey, mouse, rat, rabbit

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

Varied amounts of mouse brain tissue lysate: 10 µg/lane (1), 5 µg/lane (2), 2.5/lane µg (3), 1.25 µg/lane (4) were extracted and separated on SDS-PAGE and blotted to a membrane. Primary antibodies were used at a dilution of 1: 4000 (0.25 µg/ml). Reaction was developed by chemiluminescence.

Additional information

Additional information 10mM PBS, pH 7,2, with 1% BSA and 0,05% sodium azide

This antibody recognizes both the native and denatured forms of Beta-tubulin

Background

Background Beta-tubulin is a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism. Tubulin is expressed at high levels in almost all tissues and cell lines. This makes it ideal to use as a loading control in Western blot.

Product citations

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immunogen:	Synthetic peptide derived from the N-terminal of the human beta-tubulin
Sub class:	IgG2a
Host:	Mouse
Clonality:	Monoclonal
Purity:	IgG2a purified on Protein A n 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
Format:	Lyophilized
Quantity:	100 µg
storage:	Store at -20°C for 3 years or more, Reconstitute with distilled water to desired concentration before use, Aliquot to avoid repeated freeze-thaw cycles, Store aliquots at 4°C for several days to weeks
tested applications:	Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
recommended dilution:	1: 2000 - 1: 5000 (WB), for other techniques end user needs to determine most optimal diltion
calculated \| apparent molecular mass [kDa]:	50 kDa
Confirmed reactivity:	Chicken, human, monkey, mouse, rat, rabbit
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	Application example Varied amounts of mouse brain tissue lysate: 10 µg/lane (1), 5 µg/lane (2), 2.5/lane µg (3), 1.25 µg/lane (4) were extracted and separated on SDS-PAGE and blotted to a membrane. Primary antibodies were used at a dilution of 1: 4000 (0.25 µg/ml). Reaction was developed by chemiluminescence.
additional information:	10mM PBS, pH 7,2, with 1% BSA and 0,05% sodium azide
additional information (application):	This antibody recognizes both the native and denatured forms of Beta-tubulin
background:	Beta-tubulin is a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism. Tubulin is expressed at high levels in almost all tissues and cell lines. This makes it ideal to use as a loading control in Western blot.