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Beta-tubulin, (loading control antibody), mouse monoclonal

AS20 4382 | Clonality: Monoclonal | Host: Mouse | Reactivity: Human

Beta-tubulin, (loading control antibody), mouse monoclonal in the group Antibodies for Human/Animal  / Human Proteins / Developmental biology at Agrisera AB (Antibodies for research) (AS20 4382)

DATA SHEET IN PDF

Qty: 
250
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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen Synthetic peptide derived from the N-terminal of the human beta-tubulin
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG2a
Purity Affinity purified using Protein A
Format Lyophilized
Quantity 100 µg
Storage

Store at -20°C for 3 years or more. Reconstitute with distilled water to desired concentration before use. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.

Tested applications Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1: 2000 - 1: 5000 (WB), for other techniques end user needs to determine most optimal diltion
Expected | apparent MW 50 kDa

Reactivity

Confirmed reactivity Chicken, human, monkey, mouse, rat, rabbit
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example


Western blot using anti-beta tubulin antibodies

Varied amounts of mouse brain tissue lysate: 10 µg/lane (1), 5 µg/lane (2), 2.5/lane µg (3), 1.25 µg/lane (4) were extracted and separated on SDS-PAGE and blotted to a membrane. Primary antibodies were used at a dilution of 1: 4000 (0.25 µg/ml). Reaction was developed by chemiluminescence. 

Additional information

Additional information 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide
This antibody recognizes both the native and denatured forms of Beta-tubulin.

Related products

Related products AS16 4027 | Anti-beta-Actin, (cytoplasmic 1), rabbit antibodies
AS20 4381 | Anti-Beta-Actin, (loading control antibody), mouse antibodies

Background

Background Beta-tubulin is a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism.  Tubulin is expressed at high levels in almost all tissues and cell lines. This makes it ideal to use as a loading control in Western blot.

Product citations

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